本文選題：紅色 + 毛癬菌�。� 參考：《中國協(xié)和醫(yī)科大學(xué)》2008年博士論文

【摘要】： 1.魯比切克毛癬菌,也稱作紅色毛癬菌魯比切克變種,1981年由Kane首先描述為一種新的皮膚癬菌,后經(jīng)證實是紅色毛癬菌的變種之一。我們從一名男性股癬患者的皮損處分離并鑒定一株魯比切克毛癬菌。方法皮屑直接鏡檢證實真菌感染,培養(yǎng)后進(jìn)行菌落及鏡下形態(tài)學(xué)觀察,生化實驗、擴(kuò)增真菌rDNA的ITS區(qū)并進(jìn)行序列測定的方法鑒定菌株。結(jié)果菌株在生理學(xué)和形態(tài)學(xué)上有以下幾個方面特征符合魯比切克毛癬菌的特征:(1)SDA上為絨毛狀菌落,PDA上產(chǎn)生紅色色素,菌落中央有紫色色素顆粒形成;(2)梨形或棒狀小分生孢子,鉛筆狀大分生孢子,正常條件下培養(yǎng)可產(chǎn)生豐富的節(jié)孢子;(3)尿素酶試驗陽性,毛發(fā)穿孔試驗陰性;(4)擴(kuò)增菌株rDNA的ITS1-4區(qū),測序后與已知紅色毛癬菌和魯比切克毛癬菌序列進(jìn)行blast比對,顯示完全一致。結(jié)論該致病菌株為中國首次分離并鑒定的皮膚癬菌-魯比切克毛癬菌。 2.通過糖皮質(zhì)激素干預(yù)改變豚鼠的免疫狀態(tài),使其不能有效地清除接種的紅色毛癬菌,從而建立紅色毛癬菌感染豚鼠的動物模型。方法自臨床標(biāo)本選取3個不同的紅色毛癬菌菌株,分別為短絨毛狀菌落、粉末狀菌落和魯比切克毛癬菌(短絨毛狀菌落,尿素酶實驗陽性)。接種前3天開始,每日1次給予曲安奈德注射液20mg/kg·d股部肌肉注射預(yù)處理,接種后給予地塞米松注射液7.5mg/kg腹腔注射,后每隔日重復(fù)注射1次,共4次追加應(yīng)用的方法,促進(jìn)紅色毛癬菌感染動物模型的建立;采用直接鏡檢、真菌培養(yǎng)和組織病理的方法驗證感染結(jié)果。結(jié)果在應(yīng)用適當(dāng)劑量和途徑的糖皮質(zhì)激素的干預(yù)后,紅色毛癬菌動物接種后6天開始出現(xiàn)直接鏡檢陽性,至第8天,16只紅色毛癬菌感染的實驗動物直接鏡檢均為陽性。感染后第10天,直接鏡檢持續(xù)陽性,毛發(fā)內(nèi)亦見菌絲或孢子,真菌培養(yǎng)均可見與接種菌相同的菌落。組織病理顯示:毛囊漏斗部見菌絲和孢子,毛根內(nèi)見菌絲和孢子。結(jié)論糖皮質(zhì)激素干預(yù)下紅色毛癬菌可感染豚鼠建立動物模型。 3.研究不同的紅色毛癬菌對動物毛發(fā)的降解作用。方法:采用體外液體培養(yǎng)紅色毛癬菌和魯比切克毛癬菌,加入數(shù)根小鼠或豚鼠毛發(fā),以低濃度的酵母浸膏提供菌株生存所必需的營養(yǎng),菌株生長所需的碳源和氮源由菌株分解動物毛發(fā)獲得,培養(yǎng)2周后觀察菌落的生長情況及動物毛發(fā)結(jié)構(gòu)的改變,須癬毛癬菌作為陽性對照。結(jié)果:2周后,紅色毛癬菌、魯比切克毛癬菌和須癬毛癬菌在豚鼠和小鼠毛發(fā)上均能生長,菌落生長后,光鏡下可見動物毛發(fā)正常結(jié)構(gòu)破壞,橫紋變模糊消失。結(jié)論:紅色毛癬菌和須癬毛癬菌一樣能夠在體外分解豚鼠或小鼠的毛發(fā)作為營養(yǎng),維持菌落的生長。
[Abstract]:1. Trichophyton rubychii, also known as rubesic variety, was first described as a new dermatophytes by Kane in 1981, and later proved to be one of the varieties of Trichophyton rubrum. A strain of Trichophyton rubyceticus was isolated and identified from the lesions of a male patient with tinea femoris. Methods the fungal infection was confirmed by direct microscopical examination of dander. The colony and morphology were observed after culture. The ITS region of fungal rDNA was amplified by biochemical experiments and the strains were identified by sequencing. Results the physiological and morphological characteristics of the strain were in accordance with the characteristics of Trichophyton rubychii. The SDA of Tinea rubestris was a fluffy colony PDA with red pigment, and purple pigment granules formed in the center of the colony to form a Pyriform or rod-shaped microconidium. The pencil-shaped macroconidium, cultured under normal conditions, produced abundant sarcosporum 3) urease positive, hair perforation test negative nil 4) to amplify the ITS1-4 region of the strain rDNA. The sequence was compared with the known sequences of Trichophyton rubrum and Trichophyton rubyceticus by blast. Conclusion this strain is the first dermatophytes isolated and identified in China. 2. The immune state of guinea pigs was changed by glucocorticoid intervention, which could not effectively eliminate the inoculated Trichophyton rubrum, thus establishing the animal model of guinea pigs infected with Trichophyton rubrum. Methods three different strains of Trichophyton rubrum were selected from clinical specimens. They were short villous colony, powdery colony and Trichophyton rubyceticus (short villous colony, positive for urease test). Three days before inoculation, triamcinolone acetonide injection 20mg/kg d was injected intramuscularly once a day. After inoculation, dexamethasone injection 7.5mg/kg was injected intraperitoneally and repeated every other day for 4 times. To promote the establishment of animal model of Trichophyton rubrum infection, direct microscopic examination, fungal culture and histopathology were used to verify the results of infection. Results after the appropriate dose and route of glucocorticoid intervention, the direct microscopic examination of Trichophyton rubrum began to appear 6 days after inoculation, and 16 experimental animals infected by Trichophyton rubrum were all positive under direct microscope on the 8th day. On the 10th day after infection, direct microscopic examination continued to be positive, mycelium or spores were also found in hair, and the same colony was found in fungal culture. Histopathology showed hypha and spores in the funnel of hair follicles and hyphae and spores in hairy roots. Conclusion the animal model of guinea pigs infected with Trichophyton rubrum can be established by glucocorticoid intervention. 3. To study the degradation effect of different Trichophyton rubrum on animal hair. Methods: Trichophyton rubrum and Trichophyton rubychii were cultured in liquid medium in vitro, and several mouse or guinea pig hairs were added to provide the necessary nutrition for the survival of the strain with a low concentration of yeast extract. The carbon and nitrogen sources needed for the growth of the strain were obtained from the decomposition of animal hair by the strain. After 2 weeks of culture, the growth of the colony and the changes of the hair structure of the animal were observed. Results after 2 weeks, Trichophyton rubestris, Trichophyton rubychii and Trichophyton tubuloides could all grow in the hair of guinea pigs and mice. After colony growth, the normal structure of animal hair was destroyed and the cross striation disappeared under light microscope. Conclusion: both Trichophyton rubrum and Trichophyton rubrum can decompose guinea pig or mouse hair as nutrition and maintain colony growth in vitro.
【學(xué)位授予單位】：中國協(xié)和醫(yī)科大學(xué)
【學(xué)位級別】：博士
【學(xué)位授予年份】：2008
【分類號】：R-332;R756

【引證文獻(xiàn)】

相關(guān)碩士學(xué)位論文 前1條

1 李海洋;核桃青皮有效化學(xué)成分及抑菌性研究[D];西北農(nóng)林科技大學(xué);2012年

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本文編號：1971271