本文選題：Neurexin + Neuroligin基因�。� 參考：《山東大學(xué)》2010年碩士論文

【摘要】： 目的：探討Neurexin和Neuroligin基因在人類及鼠類動(dòng)物腸神經(jīng)系統(tǒng)(ENS)是否有表達(dá),以及對(duì)ENS突觸結(jié)構(gòu)與功能的作用,探查其在先天性巨結(jié)腸各段的表達(dá)特征及意義。 方法：首先制備小鼠、大鼠、豚鼠、正常人體腸管肌間神經(jīng)叢鋪片,通過雙重免疫熒光細(xì)胞染色,證明Neurexin、Neuroligin基因是否在腸神經(jīng)系統(tǒng)表達(dá),再采用免疫組織化學(xué)技術(shù)對(duì)20例先天性巨結(jié)腸狹窄段,移行段和擴(kuò)張段肌間神經(jīng)叢鋪片標(biāo)本染色,判斷Neurexin、Neuroligin基因的表達(dá)情況。結(jié)果：(1)Neurexin、Neuroligin蛋白在小鼠、大鼠、豚鼠、人體的腸肌間神經(jīng)叢與神經(jīng)細(xì)胞標(biāo)記Hu在同一細(xì)胞表達(dá)；而Neurexin蛋白與突觸前標(biāo)記物synaptophysin, Neuroligin蛋白與突觸后標(biāo)記物PSD95也在同一神經(jīng)組織出現(xiàn)。(2) Neurexin、Neuroligin蛋白在先天性巨結(jié)腸擴(kuò)張段表達(dá)最多,移行段明顯減少(P0.05),病變段無表達(dá)；同一組織Neurexin和Neuroligin的表達(dá)無明顯差異(P＞0.05)。 結(jié)論：Neurexin、Neuroligin基因在鼠齒類動(dòng)物及人體腸神經(jīng)系統(tǒng)都有表達(dá),不僅在胞體,而且在軸突、樹突都有表達(dá),Neurexin在突觸前表達(dá),Neuroligin在突觸后表達(dá)。它們對(duì)腸神經(jīng)系統(tǒng)突觸的結(jié)構(gòu)功能起重要作用,與一些胃、腸道動(dòng)力性疾病有密切關(guān)系,是導(dǎo)致先天性巨結(jié)腸蠕動(dòng)功能喪失的重要原因。
[Abstract]:Aim: to investigate the expression of Neurexin and Neuroligin genes in the intestinal nervous system of human and rodent, and to explore the expression characteristics and significance of ENS synaptic structure and function in Hirschsprung's disease (Hirschsprung's disease). Methods: first of all, we prepared mice, rats, guinea pigs and normal human intestinal intermuscular plexus. The expression of Neurexin Neuroligin gene in the enteric nervous system was proved by double immunofluorescence staining. Immunohistochemical staining was used to detect the expression of Neurexin Neuroligin gene in 20 specimens of myenteric plexus of Hirschsprung's disease. Results the expression of Neurexin Neuroligin protein in mouse, rat, guinea pig, human intestinal myenteric plexus and nerve cell labeled Hu was found in the same cell. Neurexin protein and presynaptic marker synaptophysin, Neuroligin protein and postsynaptic marker PSD95 were also found in the same nerve tissue.) Neurexin Neuroligin protein was most expressed in the dilated segment of Hirschsprung's disease. There was no significant difference between the expression of Neurexin and Neuroligin in the same tissue (P > 0.05). Conclusion the expression of Neurexin Neuroligin gene is found in both mouse dentin and human intestinal nervous system. It is expressed not only in the cell body but also in the axons and dendrites. Neurexin is expressed in presynaptic cells and neuroligin is expressed in postsynaptic system. They play an important role in the synaptic structure and function of the intestinal nervous system, and are closely related to some gastric and intestinal dynamic diseases, which are the important reasons for the loss of peristaltic function in Hirschsprung's disease.
【學(xué)位授予單位】：山東大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2010
【分類號(hào)】：R341

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本文編號(hào)：1959843