本文選題：腦缺血損傷 + 亞低溫�。� 參考：《中南大學(xué)》2013年碩士論文

【摘要】：目的：本課題通過(guò)觀察研究局部亞低溫治療對(duì)不同腦缺血模型自體神經(jīng)干細(xì)胞的影響,為今后干細(xì)胞的聯(lián)合治療提供實(shí)驗(yàn)依據(jù)。 方法：108只SD大鼠分為假手術(shù)、缺血再灌注(tMACO)、永久阻塞(pMACO)、tMACO+亞低溫、pMACO+亞低溫五組,各組根據(jù)時(shí)間點(diǎn)分為缺血6h、2d、7d三個(gè)亞組。線栓法建立大鼠腦缺血模型,亞低溫組栓塞成功后即刻予3h的頭部亞低溫治療(32-35℃)。記錄治療過(guò)程中動(dòng)物直腸溫度,評(píng)估缺血前、缺血后6h、2d、7d動(dòng)物一般情況、神經(jīng)功能缺損評(píng)分、梗死體積及brdu+、nestin+、brdu+/nestin+細(xì)胞數(shù)。 結(jié)果：術(shù)后6h,tMACO組神經(jīng)功能缺損評(píng)分明顯高于tMACO+亞低溫組,p=0.039,pMACO+亞低溫組明顯高于tMACO+亞低溫組,p=0.027；各實(shí)驗(yàn)組術(shù)后7d的神經(jīng)功能缺損評(píng)分均低于術(shù)后6h,p0.05；各實(shí)驗(yàn)組不同時(shí)間點(diǎn)梗死體積無(wú)差異；各實(shí)驗(yàn)組brdu+、nestin+、 brdu+/nestin+細(xì)胞于術(shù)后2天開(kāi)始增加,術(shù)后7天的陽(yáng)性細(xì)胞數(shù)目明顯高于術(shù)后6h,p0.05,而實(shí)驗(yàn)組之間陽(yáng)性細(xì)胞數(shù)目差異無(wú)統(tǒng)計(jì)學(xué)意義；tMACO+亞低溫組術(shù)后2d、7d皮層中nestin+細(xì)胞數(shù)高于tMACO組,pMACO+亞低溫組術(shù)后2d皮層中nestin+細(xì)胞數(shù)高于tMACO組。 結(jié)論：腦缺血損傷可刺激自體神經(jīng)干細(xì)胞增殖；短暫輕度亞低溫治療可促進(jìn)自體神經(jīng)干細(xì)胞向損傷區(qū)遷徙；亞低溫聯(lián)合再灌注的腦保護(hù)作用優(yōu)于亞低溫聯(lián)合永久缺血,但優(yōu)勢(shì)短暫。圖12幅,表7個(gè),參考文獻(xiàn)53篇。
[Abstract]:Objective: to study the effect of local mild hypothermia on neural stem cells in different cerebral ischemia models, and provide experimental evidence for the combined treatment of stem cells in the future. Methods one hundred and eight Sprague-Dawley rats were divided into five groups: sham operation, ischemia reperfusion, permanent occlusion, mild hypothermia and mild hypothermia. According to the time points, each group was divided into three subgroups. A rat model of cerebral ischemia was established by thread embolization. The mild hypothermia group was treated with mild hypothermia for 3 hours at 32-35 鈩，

本文編號(hào)：1954150