本文選題：脂細(xì)胞 + LRP16��； 參考：《中國人民解放軍軍醫(yī)進(jìn)修學(xué)院》2009年碩士論文

【摘要】： LRP16是從健康成人外周血淋巴細(xì)胞中克隆的人類基因,它在多種雌激素依賴性腫瘤中高表達(dá)。近年腫瘤組織的代謝成為研究熱點(diǎn),一些促癌基因,并不一定起著促進(jìn)增殖、抑制凋亡的作用,它們可能通過改變腫瘤組織的代謝模式使其適應(yīng)乏氧、缺少營養(yǎng)物質(zhì)供給的環(huán)境,從而生存下去。這提示我們,促癌基因也有可能影響機(jī)體的代謝模式——目前也已有這方面的證據(jù)。另外已經(jīng)證實(shí),一些肥胖調(diào)節(jié)基因在腫瘤發(fā)生方面起著一定的作用。因此我們假設(shè)腫瘤和肥胖可能存在“共同起源”,即某些基因的過度激活(或抑制)可能會(huì)導(dǎo)致同時(shí)發(fā)生腫瘤和肥胖。LRP16作為與雌激素作用相關(guān)的促癌基因,很可能在機(jī)體的營養(yǎng)物質(zhì)代謝方面也扮演一定的角色。所以本課題希望在細(xì)胞層面上證明LRP16對(duì)脂肪細(xì)胞分化、胰島素抵抗等方面有影響。 本研究共分為3部分: 一:應(yīng)用基因芯片技術(shù)研究LRP16基因?qū)Τ墒熘炯?xì)胞功能影響目的:探討LRP16基因?qū)Τ墒熘炯?xì)胞功能影響及其潛在的臨床意義。方法:(1)經(jīng)典脂肪細(xì)胞誘導(dǎo)方案誘導(dǎo)前脂肪細(xì)胞為成熟脂肪細(xì)胞。(2)應(yīng)用高通量基因芯片分析技術(shù),通過比較高表達(dá)LRP16的成熟脂肪細(xì)胞與正常表達(dá)組差異表達(dá)的基因,分析LRP16可能涉及的信號(hào)通路和靶基因,為進(jìn)一步研究確立方向。結(jié)果:實(shí)驗(yàn)組與對(duì)照組間差異表達(dá)基因共有1222個(gè),其中653個(gè)上調(diào),569個(gè)下調(diào)。其中涉及多個(gè)與脂肪分化、胰島素抵抗以及糖脂代謝相關(guān)的信號(hào)通路。結(jié)論:對(duì)脂肪細(xì)胞而言,LRP16總體的功能是促進(jìn)(微)炎癥狀態(tài),對(duì)脂肪分化、胰島素抵抗以及糖脂代謝都有影響。 二:LRPl6基因可能通過促進(jìn)PGC-1α的表達(dá)增強(qiáng)細(xì)胞的能量代謝目的與方法:通過質(zhì)粒轉(zhuǎn)染使3T3-L1脂肪細(xì)胞過表達(dá)LRP16(3T3-16)。通過油紅O染色,在顯微鏡下觀察LRP16對(duì)脂肪細(xì)胞脂滴形成的影響。使用Real-time PCR技術(shù)檢測3T3-16與3T3-3.1(對(duì)照組)兩組脂肪細(xì)胞與產(chǎn)熱有關(guān)的基因mRNA的相對(duì)表達(dá)量。結(jié)果:脂肪細(xì)胞誘導(dǎo)成熟后,使用油紅O染色,經(jīng)倒置顯微鏡觀察:3T3-16組細(xì)胞著色脂滴明顯少于3T3-3.1組。過表達(dá)LRP16組其過氧化物酶體增殖物激活受體共激活因子-1α、解偶聯(lián)蛋白2以及肌肉型肉毒堿棕櫚酰轉(zhuǎn)移酶1的mRNA水平表達(dá)上調(diào)。結(jié)論:LRP16可能具有使白色脂肪細(xì)胞的能量代謝向棕色脂肪細(xì)胞特性轉(zhuǎn)變的作用。三:LRP16基因?qū)χ疽蜃颖磉_(dá)的影響 目的:探討LRP16基因?qū)χ疽蜃拥挠绊�。方�?使用Real-time PCR技術(shù)檢測兩組脂肪細(xì)胞的脂聯(lián)素和瘦素mRNA的相對(duì)表達(dá)量。結(jié)果:過表達(dá)LRP16組其脂聯(lián)素和瘦素mRNA水平表達(dá)上調(diào)。結(jié)論:LRP16基因在整體層面上可能有促進(jìn)能量代謝、改善糖耐量等的作用。 結(jié)論 1.LRP16對(duì)脂肪細(xì)胞總體的效應(yīng)可能是促進(jìn)(微)炎癥狀態(tài),對(duì)脂肪分化、胰島素抵抗以及糖脂代謝都有影響。 2.LRP16可能具有使白色脂肪細(xì)胞的能量代謝向棕色脂肪細(xì)胞特性轉(zhuǎn)變的作用,在細(xì)胞層面上促進(jìn)能量代謝。 3.LRP16基因在整體層面上可能有促進(jìn)能量代謝、改善糖耐量等的作用。以上研究結(jié)果表明:LRP16在脂肪細(xì)胞的代謝方面發(fā)揮著重要的作用。
[Abstract]:LRP16 is a human gene cloned from the peripheral blood lymphocytes of healthy adults. It is highly expressed in a variety of estrogen dependent tumors. In recent years, the metabolism of tumor tissues has become a hot spot. Some of the oncogenes do not necessarily play a role in promoting proliferation and inhibiting apoptosis. They can be adapted to adapt the metabolic patterns of tumor tissues to adapt them. Hypoxia, the absence of an environment for the supply of nutrients, can survive. This suggests that the oncogene may also affect the metabolic pattern of the body - and there is now evidence for this. In addition, some obesity regulatory genes have been shown to play a role in the occurrence of cancer. "Common origin", that is, the overactivation (or inhibition) of certain genes may lead to the simultaneous occurrence of tumor and obesity.LRP16 as a oncogene associated with the role of estrogen, and is likely to play a role in the metabolism of nutrients in the body. Therefore, this topic hopes to demonstrate the differentiation of LRP16 on the adipocytes at the cellular level. There is an impact on Isle resistance.
This study is divided into 3 parts:
1: To study the effect of LRP16 gene on the function of mature adipocyte using gene chip technology: To explore the effect of LRP16 gene on the function of mature adipocytes and its potential clinical significance. Methods: (1) the preadipocyte before induction of classical adipocyte induction is mature adipocytes. (2) high throughput gene chip analysis technique, through comparison The differentially expressed genes expressed in the mature adipocytes of LRP16 and the normal expression group, analyzed the possible signal pathways and target genes that LRP16 might involve, and established the direction for further study. Results: there were 1222 differentially expressed genes between the experimental group and the control group, of which 653 were up and 569 down-regulated. Among them, many were associated with fat differentiation and insulin. Resistance and glycolipid signaling pathways. Conclusion: for adipocytes, the overall function of LRP16 is to promote (micro) inflammatory state and affect fat differentiation, insulin resistance, and glycolipid metabolism.
Two: the LRPl6 gene may enhance the energy metabolism of the cells by promoting the expression of PGC-1 A: transfection of 3T3-L1 adipocytes to LRP16 (3T3-16) through plasmid transfection. The effect of LRP16 on lipid droplet formation was observed under microscope by oil red O. Real-time PCR technique was used to detect 3T3-16 and 3T3-3.1 (control group) two. Results: the relative expression of adipocytes and heat producing gene mRNA. Results: after the adipocytes were induced to mature, the fat cells were stained with oil red O and observed by inverted microscope: the coloured lipid droplets in the 3T3-16 group were significantly less than that of the 3T3-3.1 group. The peroxisome proliferator activated receptor co activator -1 alpha, uncoupling protein 2 and muscle were expressed in the LRP16 group. The mRNA expression of botuline palmityl transferase 1 is up-regulated. Conclusion: LRP16 may have the role of energy metabolism in white adipocytes to the transformation of brown adipocytes. Three: the effect of LRP16 gene on the expression of fat factor
Objective: To investigate the effect of LRP16 gene on adipose factors. Methods: Real-time PCR technique was used to detect the relative expression of adiponectin and leptin mRNA in two groups of adipocytes. Results: the expression of adiponectin and leptin mRNA in the LRP16 group was up-regulated. Conclusion: the LRP16 gene may promote energy metabolism and improve the glucose tolerance on the whole layer. The role.
conclusion
The overall effect of 1.LRP16 on adipocytes may be to promote (micro) inflammatory state, and to affect fat differentiation, insulin resistance and glucose and lipid metabolism.
2.LRP16 may play a role in transforming the energy metabolism of white adipocytes into brown adipocytes, and promote energy metabolism at the cellular level.
The 3.LRP16 gene may have the role of promoting energy metabolism and improving glucose tolerance at the overall level. The above results show that LRP16 plays an important role in the metabolism of adipocytes.
【學(xué)位授予單位】：中國人民解放軍軍醫(yī)進(jìn)修學(xué)院
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2009
【分類號(hào)】：R329

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