本文選題：中華按蚊 + CYP6　； 參考：《重慶師范大學(xué)》2014年碩士論文

【摘要】：蚊媒傳染病嚴(yán)重威脅公眾健康，其中瘧疾危害最為嚴(yán)重。僅2012年，全球就有2.07億人感染瘧疾，，造成約62.7萬人死亡。噴灑殺蟲劑以及懸掛經(jīng)殺蟲劑浸泡的蚊帳為防控瘧疾最常用手段。自上世紀(jì)50年代開始，各類殺蟲劑被用于媒介蚊蟲的控制，然而隨著殺蟲劑的廣泛使用，在全球多達(dá)64個(gè)國(guó)家已發(fā)現(xiàn)蚊蟲對(duì)殺蟲劑產(chǎn)生抗藥性。蚊蟲對(duì)殺蟲劑產(chǎn)生抗藥性的主要原因是解毒酶的過量表達(dá)。P450是蚊蟲體內(nèi)3大類解毒酶之一，CYP6家族基因與殺蟲劑抗藥性密切相關(guān)。鑒定蚊蟲抗藥性相關(guān)基因，研究其功能，闡釋蚊蟲抗藥性分子機(jī)理對(duì)于蚊媒疾病的有效控制具有指導(dǎo)意義。 本研究利用HMM和雙向Blast的方法鑒定了中華按蚊CYP6家族基因，對(duì)其基因結(jié)構(gòu)和特征進(jìn)行了生物信息學(xué)分析，并對(duì)安徽、云南和重慶的對(duì)溴氰菊酯敏感和產(chǎn)生抗藥性的中華按蚊構(gòu)建的表達(dá)譜進(jìn)行了初步分析，得到的主要結(jié)論如下： ①在中華按蚊中共鑒定出22個(gè)CYP6家族基因。中華按蚊基因組中鑒定出的22個(gè)CYP6家族基因分屬于CYP6AA、CYP6AD、CYP6AF、CYP6AG、CYP6AH、CYP6M、CYP6N、CYP6P、CYP6R、CYP6Y和CYP6Z這11個(gè)亞家族。 ②中華按蚊CYP6家族基因與岡比亞按蚊CYP6家族基因內(nèi)含子數(shù)目和內(nèi)含子型相同。除CYP6AG和CYP6AH亞家族之外的9個(gè)亞家族僅含有1個(gè)相位1型內(nèi)含子；而CYP6AG亞家族含有3個(gè)內(nèi)含子，內(nèi)含子1為相位2型內(nèi)含子，內(nèi)含子2為相位0型內(nèi)含子，內(nèi)含子3為相位1型內(nèi)含子，CYP6AH亞家族則含有4個(gè)內(nèi)含子，分別為相位0型、相位2型、相位0型和相位1型內(nèi)含子。 ③中華按蚊基因組CYP6家族有2個(gè)基因簇。中華按蚊基因組數(shù)據(jù)分析顯示CYP6Y亞家族基因與AsCYP6N3、AsCYP6R1構(gòu)成了1個(gè)基因簇；中華按蚊中AsCYP6AD1與AsCYP6P4、AsCYP6P1和AsCYP6P2構(gòu)成了另1個(gè)簇。 ④中華按蚊表達(dá)譜中CYP6AA1、CYP6AA2、CYP6M2、CYP6P3、CYP6P5和CYP6Z1有差異表達(dá)。CYP6P3和CYP6P5在安徽的抗藥性雌蚊中上調(diào)表達(dá)，CYP6AA1、CYP6AA2、CYP6P3和CYP6P5在云南的抗藥性雌蚊中上調(diào)表達(dá)，CYP6M2和CYP6Z1在重慶的抗藥性雌蚊中下調(diào)表達(dá)。
[Abstract]:Mosquito-borne infectious diseases pose a serious threat to public health, of which malaria is the most serious. In 2012 alone, 207 million people worldwide were infected with malaria, killing about 627000 people. Spraying insecticides and hanging insecticide-soaked mosquito nets are the most common means of malaria control. Since the 1950s, various insecticides have been used for vector mosquito control. However, with the widespread use of insecticides, mosquitoes have been found to be resistant to insecticides in up to 64 countries around the world. The main reason for mosquito resistance to insecticides is that the over-expression of detoxifying enzymes. P450 is one of the three major detoxifying enzymes in mosquitoes. CYP6 family genes are closely related to insecticide resistance. The identification of mosquito resistance related genes, the study of their function, and the elucidation of the molecular mechanism of mosquito resistance have guiding significance for the effective control of mosquito-borne diseases. In this study, the genes of CYP6 family of Anopheles sinensis were identified by HMM and two-way Blast, and their gene structure and characteristics were analyzed by bioinformatics. The expression profiles of Anopheles sinensis which were sensitive to deltamethrin and resistant to drug were preliminarily analyzed in Yunnan and Chongqing. The main conclusions were as follows: 1 A total of 22 CYP6 family genes were identified in Anopheles sinensis. The 22 CYP6 family genes identified from the genome of Anopheles sinensis belong to 11 subfamilies, CYP6AAAA-CYP6ADAG-CYP6AAGG, CYP6AHG, CYP6AHN, CYP6P6N, CYP6RGY, and CYP6Z, which belong to the 11 subfamilies of CYP6AA-Anopheles sinensis (Anopheles sinensis). 2Anopheles sinensis CYP6 family gene was the same as Anopheles gambiae CYP6 gene intron number and intron type. Except for the CYP6AG and CYP6AH subfamilies, the 9 subfamilies contained only one phase 1 intron, while the CYP6AG subfamily contained 3 introns, in which intron 1 was phase 2 intron, and intron 2 was phase 0 intron. The CYP6AH subfamily of intron 3 has four introns: phase 0, phase 2, phase 0 and phase 1. 3Genomic CYP6 family of Anopheles sinensis has two gene clusters. The genomic data analysis of Anopheles sinensis showed that the subfamily genes of CYP6Y and AsCYP6N3ACYP6R1 constituted a gene cluster, while AsCYP6AD1 and AsCYP6P4Anopheles sinensis formed another cluster. (4) CYP6AA1, CYP6AA2Anopheles sinensis and CYP6M3CYP6P5 were differentially expressed with CYP6Z1. CYP6P3 and CYP6P5 up-regulated the expression of CYP6A1CY CYP6AA2CYP6P3 and CYP6P6P6P3 and CYP6P5 down-regulated the expression of CYP6M2 and CYP6Z1 in resistant females in Chongqing.
【學(xué)位授予單位】：重慶師范大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2014
【分類號(hào)】：R384.1

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