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  本文選題：Math5 + 骨髓間充質干細胞�。� 參考：《吉林大學》2010年博士論文

【摘要】： 骨髓間充質干細胞(bone marrow mesenchymal stem cells BMSCs)具有向視網膜神經節(jié)樣細胞分化的能力,但現(xiàn)誘導條件下其分化率較低,通過外源性給予促進神經分化關鍵基因來提高BMSCs的分化效率,將是一個切實可行的方案。Math5目前被認為是鼠視網膜神經節(jié)細胞生成的關鍵基因,將Math5導入BMSCs,若能夠促進其分化,將為有效解決BMSCs的低神經分化問題提供一新的思路。 本研究采用不同方法體外誘導BMSCs分化,進一步證明其向視網膜神經節(jié)樣細胞定向分化潛能�；瘜W合成Math5并成功構建同源重組腺病毒載體,對BMSCs進行轉染。轉染后細胞穩(wěn)定表達Math5,可分化為視網膜神經節(jié)樣細胞,并且在視網膜條件分化液誘導環(huán)境下該分化能力得到一定程度的提高。這一分化過程中RT-PCR檢測顯示GAP-43和Brn-3b表達上調。 上述實驗證明BMSCs具有向視網膜神經節(jié)樣細胞定向分化的能力；成功構建的Math5腺病毒載體可高效轉染BMSCs并被表達；Math5能夠促使BMSCs向視網膜神經節(jié)樣細胞分化,在視網膜條件分化液誘導下該作用更明確；Math5發(fā)揮作用推測與激活下游GAP-43、Brn-3b有關。該研究結果為解決BMSCs的低分化率問題提供了新的研究方向,并初步揭示了Math5發(fā)揮作用的機制,為其后續(xù)應用奠定理論基礎。
[Abstract]:Bone mesenchymal stem cells (marrow mesenchymal stem cells BMSCs) have the ability to differentiate into retinal ganglion like cells (RGCs), but the differentiation rate is relatively low under the present induction condition. The differentiation efficiency of BMSCs can be improved by exogenous giving key genes to promote neural differentiation. Math5 is considered to be a key gene in the formation of rat retinal ganglion cells. The introduction of Math5 into BMSCs, if it can promote its differentiation, will provide a new way to effectively solve the problem of low nerve differentiation of BMSCs. In this study, different methods were used to induce the differentiation of BMSCs into retinal ganglion like cells (RGCs) in vitro. Math5 was chemically synthesized and the homologous recombinant adenovirus vector was successfully constructed, and BMSCs was transfected. After transfection, the cells expressed Math5 stably, which could differentiate into retinal gangliona-like cells, and the differentiation ability was improved to some extent under the condition of retinal conditioned differentiation fluid. The expression of GAP-43 and Brn-3b was up-regulated by RT-PCR during this differentiation. The results show that BMSCs has the ability to differentiate into retinal ganglion like cells, and that the successfully constructed adenovirus vector of Math5 can efficiently transfect BMSCs and be expressed to induce BMSCs to differentiate into retinal gangliona-like cells. The role of Math5 may be related to the activation of GAP-43 Brn-3b downstream under the induction of retinal conditioned differentiation fluid. The results of this study provide a new research direction for solving the problem of low differentiation rate of BMSCs, and preliminarily reveal the mechanism of the role of Math5, and lay a theoretical foundation for its subsequent application.
【學位授予單位】：吉林大學
【學位級別】：博士
【學位授予年份】：2010
【分類號】：R329

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