發(fā)布時(shí)間：2018-05-17 16:03

  本文選題：痛抑郁二聯(lián)征 + 羥色胺��； 參考：《解剖學(xué)報(bào)》2015年02期

【摘要】：目的探討痛抑郁二聯(lián)征模型大鼠中縫背核(DRN)5羥色胺(5-HT)陽性細(xì)胞的分布特點(diǎn)。方法20只雄性SD大鼠隨機(jī)分為對照組和模型組。模型組大鼠連續(xù)3d皮下注射利血平,每日1次,以誘發(fā)痛抑郁二聯(lián)征模型。模型制作后第1天和第2天檢測左后足機(jī)械縮足閾觀察大鼠痛閾變化,模型制作后第2天曠場實(shí)驗(yàn)和高架O迷宮實(shí)驗(yàn)檢測大鼠情緒變化,免疫熒光技術(shù)觀察DRN前囟后6.8mm、7.3mm和7.8mm水平的5-HT表達(dá)。結(jié)果模型組大鼠經(jīng)利血平注射后機(jī)械痛閾顯著下降,并產(chǎn)生抑郁樣行為。對照組的DRN前囟后6.8mm、7.3mm和7.8mm水平的5-HT陽性細(xì)胞表達(dá)量分別為106.00±10.21、96.67±24.50和195.67±2.33。模型組相應(yīng)的表達(dá)量分別為61.67±14.53,72.33±34.35和53.67±26.77。除前囟后7.8mm水平模型組與對照組相比有顯著性差異(P0.05)之外,其他兩個(gè)水平5-HT陽性細(xì)胞表達(dá)量模型組和對照組之間差異無顯著性(P0.05)。結(jié)論利血平誘導(dǎo)的痛抑郁二聯(lián)征大鼠模型以及中縫背核5-HT表達(dá)的降低,可能主要與DRN前囟后7.8mm水平的5-HT陽性細(xì)胞數(shù)變化有關(guān),而與前囟后6.8mm和后7.3mm水平無關(guān)。
[Abstract]:Objective to investigate the distribution of DRN5 serotonin 5 HT positive cells in dorsal raphe nucleus (DRN) of rat model of pain and depression. Methods Twenty male SD rats were randomly divided into control group and model group. Rats in the model group were subcutaneously injected reserpine once a day for 3 days to induce pain and depression. On the 1st and 2nd day after the model was made, the mechanical contraction threshold of left hind foot was measured to observe the changes of pain threshold in rats, the open field experiment and the elevated O-maze test were used to detect the changes of emotion in rats on the second day after the model was made. Immunofluorescence technique was used to detect the expression of 5-HT and 7.8mm in 6. 8 mm and 7. 3 mm of anterior fontanelle of DRN. Results the mechanical pain threshold was significantly decreased and depressive behavior was produced in model group after reserpine injection. The expression of 5-HT positive cells in the control group was 106.00 鹵10.21, 96.67 鹵24.50 and 195.67 鹵2.33, respectively. The corresponding expression levels in the model group were 61.67 鹵14.53, 72.33 鹵34.35 and 53.67 鹵26.77, respectively. There was no significant difference in the expression of 5-HT positive cells between the model group and the control group except for the model group with 7.8mm level behind the anterior fontanelle (P 0.05), and there was no significant difference between the other two levels of 5-HT positive cells expression in the model group and the control group (P 0.05). Conclusion the decrease of 5-HT expression in the rat model of pain and depression induced by reserpine and the decrease of 5-HT expression in the dorsal raphe nucleus may be related to the number of 5-HT positive cells in the 7.8mm level behind the anterior fontanelle of DRN, but not to the level of 6.8mm and post-7.3mm in the anterior fontanelle.
【作者單位】： 浙江中醫(yī)藥大學(xué)第三臨床醫(yī)學(xué)院針灸神經(jīng)生物學(xué)實(shí)驗(yàn)室;
【基金】：國家自然科學(xué)基金資助項(xiàng)目(81072855) 浙江省自然科學(xué)基金資助項(xiàng)目(Z2100979,LY12H27015)
【分類號(hào)】：R741.02;R-332

【參考文獻(xiàn)】

相關(guān)期刊論文 前4條

1 吳媛媛;趙曉蕓;蔣永亮;方劍喬;;不同頻率電針鎮(zhèn)痛抗抑郁效應(yīng)觀察和對中樞5-HT的影響[J];浙江中醫(yī)藥大學(xué)學(xué)報(bào);2014年08期

2 高永靜;趙志奇;張玉秋;;外周注射福爾馬林誘導(dǎo)大鼠前扣帶皮層中ERK-CREB的激活[J];解剖學(xué)報(bào);2006年06期

3 郁曉燕;倪衡建;高永靜;;外周炎癥性疼痛刺激誘導(dǎo)大鼠中腦導(dǎo)水管周圍灰質(zhì)膠質(zhì)細(xì)胞激活及細(xì)胞因子的表達(dá)[J];解剖學(xué)報(bào);2008年04期

4 方芳;邵曉梅;沈醉;孫晶;方劍喬;;神經(jīng)病理痛模型大鼠前扣帶皮層不同水平磷酸化細(xì)胞外信號(hào)調(diào)節(jié)激酶表達(dá)差異比較[J];解剖學(xué)報(bào);2014年05期

【共引文獻(xiàn)】

相關(guān)期刊論文 前7條

1 梁宜;方劍喬;劉U，

本文編號(hào)：1901986