本文選題：脊神經(jīng)根 + 大鼠��； 參考：《山東大學(xué)》2008年博士論文

【摘要】： 目的:神經(jīng)根牽拉引起的神經(jīng)功能異常在臨床工作中十分常見,如腰椎間盤突出癥、臂叢神經(jīng)損傷等。關(guān)于機(jī)械牽拉作用對(duì)神經(jīng)組織的研究主要集中在外周神經(jīng),以至于很多關(guān)于神經(jīng)根方面的觀點(diǎn)都是通過外周神經(jīng)的研究推論得來。外周神經(jīng)和神經(jīng)根在解剖結(jié)構(gòu)和生物力學(xué)性質(zhì)上都有明顯不同,所以牽拉神經(jīng)根出現(xiàn)的功能學(xué)和形態(tài)學(xué)變化也必然與外周神經(jīng)不同。但是,目前直接利用神經(jīng)根研究機(jī)械牽拉影響還比較少,特別是用不同的程度和速度牽拉神經(jīng)根研究其出現(xiàn)病理生理學(xué)變化還未見報(bào)道。因此我們利用生物力學(xué)的一些研究方法建立大鼠L5神經(jīng)根機(jī)械牽拉模型,設(shè)定不同的程度和速度進(jìn)行牽拉。然后通過神經(jīng)電生理的方法研究牽拉后神經(jīng)根神經(jīng)功能的變化,并且在完成測試后對(duì)神經(jīng)根進(jìn)行組織學(xué)研究,了解牽拉后神經(jīng)根的病理變化以及與神經(jīng)功能改變之間的關(guān)系。 方法:手術(shù)暴露大鼠L5神經(jīng)根,將神經(jīng)背根近端切斷并連接于Endura-Tec-3200材料測試儀,Wintest軟件控制牽拉的長度和速度。高速相機(jī)記錄神經(jīng)根標(biāo)記各點(diǎn)間的運(yùn)動(dòng),記錄的圖像通過ReadCam軟件傳輸?shù)诫娔X。Image Express軟件分析神經(jīng)根上標(biāo)記各點(diǎn)在圖像中的位移,計(jì)算出牽拉神經(jīng)根實(shí)際牽拉程度。神經(jīng)根牽拉前后10分鐘內(nèi),進(jìn)行神經(jīng)電生理測試。在暴露的同側(cè)坐骨神經(jīng)下方放置刺激電極,L5神經(jīng)背根下放置兩個(gè)記錄電極。分別以0-3V刺激激活神經(jīng)復(fù)合動(dòng)作電位。獲得的信號(hào)記錄到EGAA系統(tǒng)進(jìn)行分析。利用神經(jīng)傳導(dǎo)速度、復(fù)合神經(jīng)動(dòng)作電位波幅的峰值、動(dòng)作電位曲線下的面積三個(gè)指標(biāo)評(píng)價(jià)神經(jīng)根牽拉前后以及不同的牽拉速度和程度對(duì)神經(jīng)功能的影響。電生理實(shí)驗(yàn)結(jié)束后取L5神經(jīng)背根進(jìn)行形態(tài)學(xué)觀察。銀浸染色用來觀察神經(jīng)纖維撕裂和纖維之間的空隙。HE染色觀察神經(jīng)根內(nèi)血管的變化(血管破裂,出血)。另外利用β-APP免疫組織化學(xué)染色的方法來評(píng)價(jià)神經(jīng)軸突軸漿運(yùn)輸損傷。建立評(píng)分系統(tǒng)并分別對(duì)三種染色方法進(jìn)行評(píng)分。統(tǒng)計(jì)學(xué)方法:單因素的方差分析和獨(dú)立樣本的t檢驗(yàn)用來分析不同牽拉程度和速度對(duì)神經(jīng)功能和形態(tài)學(xué)的影響。Logistic回歸分析出現(xiàn)神經(jīng)傳導(dǎo)完全阻斷的牽拉閾值。同時(shí)線性回歸分析出現(xiàn)的功能學(xué)和形態(tài)學(xué)變化與牽拉程度、速度之間是否存在線形關(guān)系。P＜0.05認(rèn)為有顯著性差異。 結(jié)果:1.神經(jīng)電生理結(jié)果顯示:(1)神經(jīng)傳導(dǎo)速度的降低率隨牽拉程度和速度的增加而增加。牽拉程度R＜10%,三種速度(0.01mm/sec,1mm/sec和15mm/sec)神經(jīng)傳導(dǎo)速度降低率分別為42.8±11.5%、46±29.2%、77.1±21.5%;牽拉程度R10-20%時(shí)三種速度(同上)牽拉的降低率為77.2±19.8%、94.5±13.5%、95.2±10.8%;牽拉程度為R＞20%時(shí)三種速度牽拉均引起神經(jīng)傳導(dǎo)的完全阻斷,這時(shí)認(rèn)為神經(jīng)傳導(dǎo)降低率為100%。線性回歸分析顯示在0.01mm/sec,牽拉程度增加和傳導(dǎo)速度減少呈線性關(guān)系(R~2=0.714)。(2)Logistic回歸分析神經(jīng)傳導(dǎo)功能完全阻斷,當(dāng)R＜20%時(shí)與牽拉速度有關(guān)。當(dāng)速度分別為0.01m/sec,1mm/sec和15mm/sec時(shí)CV完全阻斷(50%occurrence)的牽拉程度分別為16%,10%,9%。(3)隨著牽拉程度的增加復(fù)合動(dòng)作電位(CAP)波幅峰值的降低率也逐漸增加,牽拉程度R＜10%,三種速度(0.01mm/sec,1 mm/sec和15mm/sec)CAP波幅峰值的降低率分別為35.8±18.7%、36.5±32.5%、85.6±28.4%;牽拉程度R10-20%時(shí)三種速度(同上)牽拉的降低率為66.2±40%、90.3±23.7%、93.5±20.6%;牽拉程度為R＞20%時(shí)三種速度牽拉CAP波幅峰值降低率為100%。直線回歸分析顯示在速度0.01mm/sec時(shí)牽拉程度和峰值降低率有線性關(guān)系(R~2=0.633)。(4)CAP曲線下面積(AUC)表示當(dāng)神經(jīng)受到電刺激后產(chǎn)生動(dòng)作電位的軸突的數(shù)量。AUC的降低率也顯示同樣的變化。牽拉程度R＜10%,三種速度(0.01mm/sec,1mm/sec,15mm/sec)AUC降低率分別為30.5±5.4%、69±18.6%、81.6±25.2%;牽拉程度R10-20%時(shí)三種速度(同上)牽拉的降低率為83.2±16%、98.9±2.6%、98.5±4.7%;牽拉程度為R＞20%時(shí)三種速度牽拉AUC均為降低率為100%。直線回歸分析顯示在速度0.01mm/sec時(shí)牽拉程度增加和AUC減低率之間存在線性關(guān)系(R~2=0.738)。 2.組織學(xué)結(jié)果顯示:(1)βAPP免疫組織化學(xué)。隨著牽拉程度和速度增加,βAPP染色陽性率也逐漸增加。正常對(duì)照組未發(fā)現(xiàn)陽性結(jié)果,假手術(shù)組染色陽性低于5%。所有被牽拉過的神經(jīng)根染色均發(fā)現(xiàn)βAPP聚集,提示在部分或全部高倍視野下有軸突損傷。牽拉程度R＜10%,三種速度(0.01 mm/sec,1 mm/sec,15mm/sec)βAPP染色的陽性率分別為8.6±4.0%、10.1±6.0%、20.8±4.7%。牽拉程度R10-20%時(shí)三種速度(同上)BAPP染色的陽性率分別為30.8±15.7%、47.6±4.1%、51±30%。牽拉程度為R＞20%時(shí)三種速度βAPP染色的陽性率分別為61±14.2%、73.4±11.2%、73.7±19.6%。直線回歸分析顯示βAPP染色陽性率和在三種不同速度時(shí)與牽拉程度均存在線性關(guān)系(0.01mm/sec,R~2=0.708,1mm/sec,R~2=0.912,15mm/sec,R~2=0.719)。(2)HE染色。HE染色用來觀察神經(jīng)根內(nèi)是否有血管破裂,提示神經(jīng)根內(nèi)是否發(fā)生病理性出血。對(duì)照組觀察到少量的血管破裂但發(fā)生率低于5%。假手術(shù)組觀察到血管破裂,發(fā)生率為25%。牽拉程度R＜10%,三種速度(0.01mm/sec,1 m/sec,15 mm/sec)血管破裂率分別為32.6±12.8%、38.2±10.5%、36.6±5.9%;牽拉程度R10-20%時(shí)三種速度血管破裂率為52.4±10.6%、61.4±4.7%、62.4±6.5%;牽拉程度為R＞20%時(shí)三種速度血管破裂率為57.7±21.9%、79.3±15.3%、89.7±6.9%。直線回歸分析發(fā)現(xiàn)拉伸程度和血管破裂率之間在速度15mm/sec(R~2=0.7738)和1mm/sec(R~2=0.7692)時(shí)存在線性關(guān)系。(3)銀浸染色觀察到正常對(duì)照組形態(tài)保持完整,神經(jīng)纖維之間發(fā)現(xiàn)有空隙但是發(fā)生率低于10%。假手術(shù)組形態(tài)基本保持完整,偶爾發(fā)現(xiàn)有神經(jīng)纖維斷裂和空隙,但發(fā)生率分別低于15%和3%。牽拉程度R＜10%,三種速度(0.01mm/sec,1mm/sec,15mm/sec)染色正常率為47.3±25.4%、46.8±26.1%、45.6±18.9%;牽拉程度R10-20%時(shí)三種速度染色正常率為40.3±7.7%、29.8±37.2%、17.9±18.4%;牽拉程度為R＞20%時(shí)三種速度染色正常率為29.7±14.9%、29.6±16.6%、3.8±5.2%。直線回歸分析在速度為0.01mm/sec和1 mm/sec時(shí),牽拉程度和出現(xiàn)空隙之間無線形關(guān)系。在速度15mm/sec時(shí)有線形關(guān)系(R~2=0.488)。神經(jīng)纖維撕裂和牽拉程度,在速度0.01mm/sec(R~2=0.6108)和15mm/sec(R~2=0.6531)時(shí)存在線形關(guān)系。 結(jié)論:建立的大鼠L5神經(jīng)根牽拉模型可以有效研究神經(jīng)根在牽拉作用下的損傷機(jī)制。特別是在牽拉過程中利用高速相機(jī)采集圖像可以分析不同時(shí)間牽拉程度和力量的變化有助于進(jìn)行神經(jīng)根生物力學(xué)的分析。利用傳導(dǎo)速度,CAP波幅峰值和曲線下面積三個(gè)指標(biāo)分析神經(jīng)功能增加了神經(jīng)電生理試驗(yàn)的準(zhǔn)確性。實(shí)驗(yàn)結(jié)果證實(shí)神經(jīng)根牽拉損傷除了與牽拉程度有關(guān)外,還與牽拉時(shí)的速度有關(guān)系。隨著牽拉程度和速度的增加,神經(jīng)功能喪失也逐漸增加。與神經(jīng)功能研究類似,牽拉后神經(jīng)根的形態(tài)學(xué)變化與牽拉速度程度都有關(guān)系。說明神經(jīng)功能喪失在一定程度上是由于神經(jīng)根形態(tài)結(jié)構(gòu)變化引起,但是可能還有其它的機(jī)制也起作用,如離子通道和神經(jīng)受體系統(tǒng)。實(shí)驗(yàn)中實(shí)驗(yàn)組與假手術(shù)組比較差異并不十分明顯,因此在下一步關(guān)于神經(jīng)根的病理學(xué)研究中還需要進(jìn)一步探討。另外我們發(fā)現(xiàn)了牽拉后神經(jīng)根呈現(xiàn)彌散性損傷,該模型造成的軸突損傷與觀察到的人腦損傷出現(xiàn)的形態(tài)學(xué)變化很相似。因此,我們建立的神經(jīng)根牽拉模型也是研究中樞系統(tǒng)軸突損傷較好的體內(nèi)研究模型。
[Abstract]:Objective: neural dysfunction caused by nerve root traction is very common in clinical work, such as lumbar intervertebral disc herniation, brachial plexus injury, and so on. The study of nerve tissue is mainly focused on peripheral nerve, so that many points about nerve root are deduced from peripheral nerve. There are obvious differences in the anatomical structure and the biomechanical properties of the peripheral nerve and the nerve root, so the changes of the functional and morphological changes of the traction nerve root are also different from the peripheral nerve. However, the influence of the mechanical traction on the direct use of the nerve root is still less, especially with the different degree and speed of the traction nerve root. The changes in pathophysiology have not been reported. Therefore, we use some methods of biomechanics to establish the mechanical pull model of L5 nerve root in rats, set different degrees and speed to pull. Then, the nerve root nerve function changes after traction are studied by the method of neurophysiology, and the nerve root after the test is completed. Histological study was carried out to understand the relationship between the pathological changes of the nerve roots after traction and the changes of nerve function.
Methods: the L5 nerve root was exposed in the operation. The proximal end of the dorsal root of the nerve was severed and connected to the Endura-Tec-3200 material tester. The length and speed of the traction were controlled by the Wintest software. The motion of the nerve root markers was recorded by the high-speed camera. The recorded images were transferred to the computer.Image Express software to mark the nerve root by ReadCam software. The actual traction of the traction nerve root was calculated with the displacement in the image. The nerve electrophysiological test was performed within 10 minutes of the nerve root before and after traction. The stimulation electrodes were placed under the exposed sciatic nerve and two recording electrodes were placed under the dorsal root of the L5 nerve. The signals obtained by stimulating the active nerve compound action potential were recorded with 0-3V. The EGAA system was analyzed. The nerve conduction velocity, the peak value of the compound nerve action potential wave amplitude and the area under the action potential curve were used to evaluate the effect of the nerve roots before and after traction and the different pulling speed and degree on the nerve function. After the electrophysiological experiment, the dorsal root of the nerve was taken to observe the morphological observation of the dorsal root of the L5 nerve. The silver immersion staining was used. To observe the changes in nerve root blood vessels (vascular rupture, bleeding) by.HE staining of nerve fibers and fiber laceration. In addition, beta -APP immunohistochemical staining was used to evaluate axonal axonal transport damage. Score system was established and three staining methods were scored. Statistical method: single factor prescription Difference analysis and t test of independent samples were used to analyze the effects of different stretch and speed on neural function and morphology..Logistic regression analysis showed the traction threshold of complete blocking of nerve conduction. At the same time, the linear regression analysis showed that there was a linear relationship between the functional and morphological changes and the degree of traction, and the linear relationship between the velocity and the speed was.P < 0.05. There is a significant difference.
Results: 1. the neurophysiological results showed that (1) the reduction rate of nerve conduction velocity increased with the increase of traction degree and speed. The degree of traction was R < 10%, three kinds of velocity (0.01mm/sec, 1mm/sec and 15mm/sec) were 42.8 + 11.5%, 46 + 29.2%, 77.1 + 21.5%, respectively, and traction at R10-20%. The reduction rate was 77.2 + 19.8%, 94.5 + 13.5%, 95.2 + 10.8%, and the stretch degree was R > 20% when three kinds of speed dragging all caused the complete block of nerve conduction. At this time, the reduction rate of nerve conduction was 100%. linear regression analysis showed in 0.01mm/sec, the degree of traction increased and the decrease of conduction velocity was linear (R~2=0.714). (2) Logistic regression analysis of God The conduction function was completely blocked, when the R < 20% was related to the pull speed. When the speed was 0.01m/sec, 1mm/sec and 15mm/sec were respectively, CV completely blocked (50%occurrence) was 16%, 10%, 9%. (3) increased with the degree of traction, and the decrease rate of the amplitude of CAP wave amplitude increased gradually, and the degree of traction was R < 10%, three speed. The reduction rate of the peak amplitude of CAP amplitude (0.01mm/sec, 1 mm/sec and 15mm/sec) was 35.8 + 18.7%, 36.5 + 32.5%, 85.6 + 28.4%, and the reduction rate of the stretch was 66.2 + 40%, 90.3 + 23.7%, 93.5 + 18.7% when the degree of traction was R10-20%, and the decrease rate of CAP wave amplitude was 100%. linear regression analysis when the stretch degree was R > 100%.. There is a linear relationship between the stretch degree and the peak reduction rate at the speed of 0.01mm/sec (R~2=0.633). (4) the area under the CAP curve (AUC) indicates that the reduction rate of the number of.AUC of the axon of the action potential when the nerve is stimulated by electrical stimulation also shows the same change. The stretch degree is R < 10%, and the three kinds of velocity (0.01mm/sec, 1mm/sec, 15mm/sec) AUC reduction rate The difference is 30.5 + 5.4%, 69 + 18.6%, 81.6 + 25.2%, and the reduction rate of three kinds of pullup is 83.2 + 16%, 98.9 + 2.6%, 98.5 + 4.7% when the stretch degree is R10-20%, and the reduction rate of AUC is 100%. linear regression analysis when the stretch degree is R > 69. Sexual relations (R~2=0.738).
2. histological results showed: (1) beta APP immuno histochemistry. With the increase of traction and speed, the positive rate of beta APP staining increased gradually. No positive results were found in the normal control group. The positive staining of the sham operation group was lower than that of the 5%.. The degree of traction was R < 10%, the positive rates of three kinds of velocity (0.01 mm/sec, 1 mm/sec, 15mm/sec) were 8.6 + 4%, 10.1 + 6%, and 20.8 + 4.7%. traction R10-20%, respectively, and the positive rates of three kinds of BAPP staining were 30.8 + 15.7%, 47.6 + 0.01, respectively. No 61 + 14.2%, 73.4 + 11.2%, 73.7 + 19.6%. linear regression analysis showed that there was a linear relationship between the positive rate of beta APP staining and the degree of traction at three different speeds (0.01mm/sec, R~2=0.708,1mm/sec, R~2=0.912,15mm/sec, R~2=0.719). (2) HE staining.HE staining was used to observe whether there was a vascular rupture within the nerve root, indicating whether the nerve roots were within the nerve root. In the control group, the control group observed a small amount of vascular rupture but the incidence of vascular rupture was lower than that of 5%. sham operation group. The incidence of 25%. traction was R < 10%, and the rate of vascular rupture was 32.6 + 12.8%, 38.2 + 10.5%, 36.6 + 5.9%, respectively, at the three kinds of speed (0.01mm/sec, 1 m/sec, 15 mm/sec), and the rupture of blood vessels when the traction degree was R10-20%. The rate was 52.4 + 10.6%, 61.4 + 4.7%, 62.4 + 6.5%, and the rate of vascular rupture was 57.7 + 21.9% at R > 20%, 79.3 + 15.3%, and 89.7 + 6.9%. linear regression analysis showed that there was a linear relationship between the tensile degree and the rupture rate of blood vessels at the speed of 15mm/sec (R~2=0.7738) and 1mm/sec (R~2= 0.7692). The shape of the group remained intact, but the incidence of the nerve fibers was found to be empty but the incidence of the group was less than that of the 10%. sham operation group. Occasionally, the nerve fiber fracture and the gap were found, but the incidence was lower than 15% and 3%. R < 10% respectively. The three kinds of speed (0.01mm/sec, 1mm/ sec, 15mm/sec) were 47.3 + 25.4%, 46.8 + 26.1%, 45.. 6 + 18.9%; the normal rate of three kinds of velocity dyeing at R10-20% was 40.3 + 7.7%, 29.8 + 37.2% and 17.9 + 18.4%, and the normal rate of three velocity dyeing was 29.7 + 14.9% and 17.9 + 18.4% when the traction degree was R > 20%, and when the velocity was 0.01mm/sec and mm/sec, the relationship between the stretch degree and the gap appeared. There is linear relationship (R~2=0.488) at 5mm/sec. The degree of tear and traction of nerve fibers is in line relationship at speed 0.01mm/sec (R~2=0.6108) and 15mm/sec (R~2=0.6531).
Conclusion: the established rat L5 nerve root traction model can effectively study the damage mechanism of nerve root under traction, especially in the process of pulling the image by high-speed camera to analyze the change of traction and strength at different time. It is helpful for the analysis of nerve root biomechanics. Using the conduction velocity and the peak amplitude of CAP amplitude, the peak value of nerve root can be analyzed. The accuracy of neurophysiological tests was increased by analyzing the three indexes of the area under the curve. The results showed that the nerve root traction injury was related to the pulling speed except the degree of traction, and the loss of nerve function increased with the increase of traction degree and speed. The morphological changes of the posterior nerve root are related to the degree of traction speed. It shows that the loss of nerve function is caused by the changes of the morphological structure of the nerve root to a certain extent, but there may be other mechanisms, such as the ion channel and the nervous receptor system. The experimental group is not very different from the sham operation group. Therefore, further discussion is needed in the next histopathological study of nerve roots. In addition, we found that the nerve root causes diffuse damage after traction, and the axon damage caused by this model is similar to the observed morphological changes in the human brain damage. Therefore, the nerve root traction model we established is also the central system. In vivo research model of axon injury.
【學(xué)位授予單位】：山東大學(xué)
【學(xué)位級(jí)別】：博士
【學(xué)位授予年份】：2008
【分類號(hào)】：R-332

【共引文獻(xiàn)】

相關(guān)期刊論文 前10條

1 趙雅寧;張文麗;田艷霞;高俊玲;;Edaravone對(duì)大鼠彌漫性腦創(chuàng)傷神經(jīng)運(yùn)動(dòng)功能及腦組織超微結(jié)構(gòu)的影響[J];第四軍醫(yī)大學(xué)學(xué)報(bào);2009年07期

2 楊霄鵬;李秋芳;楊瑞玲;;低分子肝素鈣治療急性腦梗死的療效觀察[J];中國實(shí)用神經(jīng)疾病雜志;2006年03期

3 楊樹東;孫榮超;梁加貝;周志毅;穆會(huì)君;芮俊;;大鼠原發(fā)性腦干損傷延腦淀粉樣β蛋白及其蛋白前體的表達(dá)[J];臨床與實(shí)驗(yàn)病理學(xué)雜志;2009年04期

4 楊樹東;孫榮超;周志毅;梁加貝;芮俊;;GFAP、NF在原發(fā)性腦干損傷后的表達(dá)及診斷價(jià)值分析[J];南京醫(yī)科大學(xué)學(xué)報(bào)(自然科學(xué)版);2009年06期

5 趙雅寧;趙毓芳;陳海紅;崔建忠;饒穎臻;高俊玲;;Edaravone對(duì)大鼠彌漫性腦創(chuàng)傷后ERK1/2通路的影響[J];神經(jīng)解剖學(xué)雜志;2009年01期

6 ;Present status of studies on diffuse axonal injury[J];Neural Regeneration Research;2006年07期

7 STEPHEN C.BONDY,CARL P.LEBEL;Oxygen Radical Generation as an Index of Neurotoxic Damage[J];Biomedical and Environmental Sciences;1991年Z1期

8 Yaning Zhao;Jianmin Li;Qiqun Tang;Junling Gao;Changxiang Chen;Liwei Jing;Pan Zhang;Shuxing Li;;Apolipoprotein E mimetic peptide protects against diffuse brain injury[J];Neural Regeneration Research;2014年05期

9 Yaning Zhao;Jianmin Li;Qiqun Tang;Pan Zhang;Liwei Jing;Changxiang Chen;Shuxing Li;;Regulation of extracellular signal-regulated kinase 1/2 influences hippocampal neuronal survival in a rat model of diabetic cerebral ischemia[J];Neural Regeneration Research;2014年07期

10 李立新,徐啟武,吳幼章,胡衛(wèi)星,顧培元,傅震;甲基強(qiáng)的松龍和腦源性神經(jīng)營養(yǎng)因子聯(lián)合使用對(duì)鼠脊髓損傷的治療作用[J];江蘇醫(yī)藥;2002年05期

相關(guān)博士學(xué)位論文 前4條

1 徐錫金;人發(fā)角蛋白促進(jìn)成年大鼠脊髓損傷修復(fù)的實(shí)驗(yàn)研究[D];中國人民解放軍第一軍醫(yī)大學(xué);2003年

2 李立新;甲基強(qiáng)的松龍和神經(jīng)干細(xì)胞移植修復(fù)脊髓損傷的實(shí)驗(yàn)研究[D];復(fù)旦大學(xué);2002年

3 張峭巍;磁共振彌散張量成像對(duì)彌漫性軸索損傷的診斷價(jià)值—?jiǎng)游飳?shí)驗(yàn)研究及臨床個(gè)例觀察[D];浙江大學(xué);2005年

4 譚浩;鈣調(diào)神經(jīng)磷酸酶在創(chuàng)傷性軸突損傷中的作用及機(jī)理[D];第三軍醫(yī)大學(xué);2006年

相關(guān)碩士學(xué)位論文 前8條

1 李雪松;大鼠彌漫腦損傷模型制作及傷后凋亡相關(guān)蛋白表達(dá)的研究[D];暨南大學(xué);2003年

2 李詠梅;TGFβ1和GDNF在脊髓全橫斷損傷后大鼠的表達(dá)[D];昆明醫(yī)學(xué)院;2005年

3 焦靜;實(shí)驗(yàn)性大鼠彌漫性腦損傷后GFAP、NO和ET的變化研究[D];鄭州大學(xué);2006年

4 劉穎;大鼠彌漫性軸索損傷伴神經(jīng)細(xì)胞凋亡和pStat1表達(dá)[D];河北醫(yī)科大學(xué);2007年

5 董麗儒;大鼠彌漫性軸索損傷神經(jīng)元、軸索繼發(fā)性改變的實(shí)驗(yàn)觀察[D];河北醫(yī)科大學(xué);2008年

6 蘇華新;濕熱型腰椎間盤突出癥患者疼痛與IgG、IgM的相關(guān)性研究[D];廣州中醫(yī)藥大學(xué);2009年

7 魏玲;大鼠腦創(chuàng)傷后PI3K/Akt信號(hào)通路參與神經(jīng)細(xì)胞凋亡的分子機(jī)制研究[D];河北醫(yī)科大學(xué);2010年

8 霍浩然;大鼠彌漫性軸索損傷模型中GPR17的表達(dá)變化及意義[D];河北醫(yī)科大學(xué);2014年

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