發(fā)布時(shí)間：2018-05-11 14:18

  本文選題：血小板源生長(zhǎng)因子 + 堿性成纖維細(xì)胞生長(zhǎng)因子　； 參考：《山東大學(xué)》2009年碩士論文

【摘要】： 在組織再生與創(chuàng)傷修復(fù)過(guò)程中,血管再生是一個(gè)非常重要的方面,它不僅可以加速組織再生,同時(shí)也是維持肉芽組織和新生組織的必要基礎(chǔ)。為了促進(jìn)血管再生的進(jìn)程,許多血管誘導(dǎo)生長(zhǎng)因子與功能生物材料在組織再生及創(chuàng)傷修復(fù)中得到廣泛應(yīng)用。血小板源生長(zhǎng)因子(Platelet-derived growth factor,PDGF)與堿性成纖維細(xì)胞生長(zhǎng)因子(basic fibroblast growth factor,bFGF)是血管誘導(dǎo)因子,能夠誘導(dǎo)內(nèi)皮細(xì)胞、血管平滑肌細(xì)胞、單核細(xì)胞、粒細(xì)胞以及纖維原細(xì)胞的增殖及遷移,在組織再生與創(chuàng)傷修復(fù)中具有重要的作用。但是,由于其在體內(nèi)易擴(kuò)散,以及缺少有效的傳輸系統(tǒng)限制了它們的臨床應(yīng)用。骨膠原(Demineralized bonematrix,DBM),即脫鈣骨基質(zhì),是目前骨組織工程中最為廣泛使用的支架,其主要成分為Ⅰ型膠原。脫鈣骨基質(zhì)除具有成骨誘導(dǎo)和成骨傳導(dǎo)作用之外,其天然孔隙及連通、合適孔隙率有利于種子細(xì)胞黏附、遷移及血管的長(zhǎng)入。 因此,為了促進(jìn)組織再生與創(chuàng)傷修復(fù)中血管再生,我們將肝素(heparin)交聯(lián)到骨膠原(HC-DBM)上,分別通過(guò)肝素特異的結(jié)合PDGF和bFGF,從而構(gòu)成PDGF和bFGF的特異的靶向傳輸系統(tǒng)。在體外的實(shí)驗(yàn)中,肝素能夠提高結(jié)合到骨膠原上生長(zhǎng)因子的量;在體外活性實(shí)驗(yàn)中,結(jié)合到膠原上的生長(zhǎng)因子能夠促進(jìn)成纖維細(xì)胞的快速增殖;在體內(nèi)皮下包埋實(shí)驗(yàn)中,結(jié)合到HC-DBM上的生長(zhǎng)因子能夠促進(jìn)細(xì)胞遷移到材料中;而且,組織學(xué)分析表明,HC-DBM能夠促進(jìn)血管再生,并能夠維持血管的穩(wěn)定�？傊�,HC-DBM能夠阻止生長(zhǎng)因子的擴(kuò)散,延長(zhǎng)其生物活性,并且能夠促進(jìn)材料的細(xì)胞化與血管化。
[Abstract]:Vascular regeneration is a very important aspect in the process of tissue regeneration and wound repair. It not only accelerates tissue regeneration, but also is the necessary basis for maintaining granulation tissue and new tissue. In order to promote the process of vascular regeneration, many vascular inducible growth factors and functional biomaterials have been widely used in tissue regeneration and wound repair. Platelet-derived growth factor (PDGFs) and basic fibroblast growth factor (basic fibroblast growth factor-bFGFs) are vascular inducible factors, which can induce proliferation and migration of endothelial cells, vascular smooth muscle cells, monocytes, granulocytes and fibroblasts. It plays an important role in tissue regeneration and wound repair. However, their clinical application is limited by their easy diffusion in vivo and the lack of effective transmission systems. Demineralized bone matrix (DBM) is the most widely used scaffold in bone tissue engineering, and its main component is type 鈪，

本文編號(hào)：1874303