發(fā)布時間：2018-05-11 07:52

  本文選題：兔 + VX2　； 參考：《泰山醫(yī)學(xué)院》2010年碩士論文

【摘要】：目的 改進(jìn)兔VX2肝癌、腎癌、乳腺癌、腦瘤模型的制作方法,使之更為簡便、省時,并且提高成瘤率。同時,探討腫瘤模型影像學(xué)表現(xiàn)。 方法 (1)VX2肝癌模型的制作新西蘭大白兔22只,隨機(jī)分成2組(每組11只),采用B超引導(dǎo)下經(jīng)皮穿刺18G針植入VX2瘤塊組織法分別接種于兔肝臟左葉(A組),兔肝臟右葉(B組);接種后1周、2周、3周、4周分別行彩超、CT和常規(guī)病理檢查。 (2)VX2腎癌模型的制作新西蘭大白兔22只,隨機(jī)分成2組(每組11只),采用B超引導(dǎo)下經(jīng)皮穿刺18G針植入VX2瘤塊組織懸液法分別接種于兔左腎(A組)及右腎(B組);接種后1周、2周、3周、4周分別行彩超、CT和常規(guī)病理檢查。 (3)VX2乳腺癌模型的制作新西蘭大白兔22只,隨機(jī)分成2組(每組11只),分別采用經(jīng)皮穿刺18G針植入VX2瘤塊組織懸液法(A組),切開局部皮膚VX2瘤塊組織移植法(B組),接種于兔胸壁左側(cè)第2乳頭墊下接種后1周、2周、3周、4周分別行彩超、CT、MR和常規(guī)病理檢查。 (4).VX2腦瘤模型的制作新西蘭大白兔1 2只,隨機(jī)分成2組(每組6只),分別采用微創(chuàng)定位顱骨鉆孔組織塊移植法(A組：取事先剪成大小約1mm3的瘤組織塊1～2塊植入預(yù)先打好的顱骨孔內(nèi))及微創(chuàng)定位顱骨鉆孔瘤組織塊懸液注入法(B組：取VX2腫瘤組織塊懸液0.3ml注射接種入預(yù)先打好的顱骨孔內(nèi))。接種后1周、2周、3周、4周分別行CT及MR檢查,觀察神經(jīng)系統(tǒng)體征變化,自然死亡后觀察組織病理學(xué)改變。 統(tǒng)計腫瘤接種成功率;觀察記錄術(shù)后并發(fā)癥：觀察腫瘤的大體表現(xiàn)及轉(zhuǎn)移情況;觀察動物自然生存情況;死亡后尸檢;腫瘤組織經(jīng)10%福爾馬林固定后,病理檢查。 結(jié)果 1.兔VX2肝癌模型：22只實驗兔發(fā)現(xiàn)瘤灶,總成瘤率72.7%(17/22),肝左葉接種組(A組)成功率90.9%(10/11),肝右葉接種組(B組)成功率為54.5%(7/11),兩組接種成功率無顯著差異, (P0.05)。 2.兔VX2腎癌模型：15只實驗兔發(fā)現(xiàn)瘤灶,總接種成功率為68.2%(15/22),左腎接種組(A組)成功率為54.5%(6/11),右腎接種組(B組)成功率為81.8%(9/11),兩組接種成功率無顯著差異,(P0.05)。 3.兔VX2乳腺癌模型：18只實驗兔發(fā)現(xiàn)瘤灶,總接種成功率為81.8%(18/22),組織塊懸液種植法接種組(A組)成功率為90.9%(10/11),組織塊移植組(B組)成功率為72.7(8/11),兩組接種成功率無顯著性差異(P0.05)。 4.兔VX2腦瘤模型：5只實驗兔發(fā)現(xiàn)瘤灶,總接種成功率為41.7%(5/12),組織塊移植組(A組)成功率為83.3%(5/6),組織塊懸液種植組(B組)成功率為0(0/6),AB兩組接種成功率有顯著性差異(P0.01)。結(jié)論 采用彩超引導(dǎo)下經(jīng)皮穿刺植入組織塊法建立兔VX2肝癌模型、兔VX2腎癌模型,采用組織塊懸液注射法建立兔VX2乳腺癌模型,采用組織塊移植法建立兔VX2腦瘤模型,使制作過程更為簡便、省時：采用彩超、CT及MR平掃對瘤灶進(jìn)行動態(tài)檢測、評價,可更為準(zhǔn)確、及時地觀察瘤灶的發(fā)生、發(fā)展等影像學(xué)改變。
[Abstract]:Purpose The method of making rabbit VX2 liver cancer, kidney cancer, breast cancer and brain tumor model was improved to make it more simple and time saving, and to increase the rate of tumorigenesis. At the same time, the imaging findings of tumor model were discussed. Method Establishment of 22 New Zealand White Rabbit models of VX2 Hepatocellular carcinoma Each group was randomly divided into two groups (11 rats in each group), each group was inoculated with percutaneous 18G needle implantation of VX2 tumor block under the guidance of B-ultrasound in Zuo Ye group A of rabbit liver and group B of right lobe of liver in rabbits respectively, and the color ultrasound was performed 1 week, 2 weeks, 3 weeks and 4 weeks after inoculation, respectively. Ct and routine pathological examination. 22 New Zealand White Rabbit models of VX2 Renal carcinoma They were randomly divided into two groups: group A (n = 11) and group B (n = 11), and group B (n = 11) received percutaneous 18G needle implantation of VX2 tumor tissue suspension under B-ultrasound guidance, respectively, and group B (n = 2) received color Doppler CT and routine pathological examination at 1 week, 2 weeks, 3 weeks and 4 weeks after inoculation. 22 New Zealand White Rabbit models of VX2 Breast Cancer Each group was randomly divided into two groups (11 rats in each group). The VX2 tumor tissue suspension was implanted with 18G needle in each group. Group B was treated with local skin VX2 tumor tissue transplantation, and inoculated under the second papilla pad on the left side of the chest wall of rabbit, and then inoculated under the second papilla pad on the left side of the chest wall. Two weeks and three weeks and four weeks were performed respectively by color Doppler imaging and conventional pathological examination. Establishment of New Zealand White Rabbit with brain tumor Model of C4X.VX2, 12 New Zealand White Rabbit, 12 New Zealand White Rabbits, 12 New Zealand White Rabbits, Randomly divided into two groups (each group, 6 rats, respectively, using minimally invasive localization of skull drilling tissue block transplantation method: group A: 1 or 2 tumor tissue blocks of about the size of 1mm3 were cut in advance to be implanted into the preformed skull foramen) and minimally invasive localization of skull drilling. Group B: VX2 tumor tissue suspension 0.3ml was injected into the skull foramen. Ct and Mr were performed 1 week, 2 weeks and 3 weeks and 4 weeks after inoculation respectively. The changes of nervous system signs and histopathological changes were observed after natural death. Statistics of success rate of tumor inoculation; observation of postoperative complications: observe the gross manifestations and metastasis of the tumor; observe the natural survival of animals; postmortem examination; tumor tissue fixed by 10% formalin, pathological examination. Result 1. The tumor foci were found in 22 rabbits with VX2 liver cancer model. The total tumorigenic rate was 72.710 / 22. The success rate of liver Zuo Ye inoculation group was 90.910 / 11a, and the success rate of liver right lobe inoculation group was 54.5g / 7 / 110.There was no significant difference between the two groups (P0.05A). 2. The tumor foci were found in 15 rabbits with VX2 renal cell carcinoma model. The total success rate of inoculation was 68.2 / 22%, the success rate of left kidney inoculation group was 54.5 / 11%, and the success rate of right kidney inoculation group was 81.8% / 11%. There was no significant difference between the two groups in the success rate of inoculation (P 0.05). 3. VX2 breast cancer model: 18 experimental rabbits found tumor foci, the total success rate of inoculation was 81.8% 22%, the success rate of group A was 90.9% 10%, and the success rate of group B was 72.7% 811%. There was no significant difference between the two groups in the success rate of inoculation (P 0.05). 4. The total success rate of VX2 brain tumor was 41.7% / 12%, the success rate was 83.3% / 6% in group A, and the success rate was 0% / 6% in group B) there was a significant difference between the two groups (P0.01%) in the VX2 brain tumor model (n = 5) and in group A (n = 5) with a total success rate of 41.7% / 12% (n = 5), and in group A (n = 5), the success rate was 83.3% (n = 5) and group B (n = 5). Conclusion Rabbit VX2 liver cancer model, rabbit VX2 renal cell carcinoma model, rabbit VX2 breast cancer model by tissue suspension injection, and rabbit VX2 brain tumor model were established by percutaneous puncture and implantation of tissue block under the guidance of color ultrasound. In order to make the process more simple and time-saving, the dynamic detection and evaluation of tumor foci by color Doppler CT and Mr plain scan can more accurately and timely observe the imaging changes such as the occurrence and development of tumor foci.
【學(xué)位授予單位】：泰山醫(yī)學(xué)院
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2010
【分類號】：R-332

【參考文獻(xiàn)】

相關(guān)期刊論文 前10條

1 胡海洋;李強(qiáng);韓志剛;康德強(qiáng);;經(jīng)皮介入微波熱凝治療實驗性VX2肺癌的有效性與安全性[J];癌癥;2007年09期

2 胡小波;曹建民;許健;陳波;;兔VX2肺癌模型的建立及評價[J];當(dāng)代醫(yī)學(xué);2009年17期

3 佘軍軍;王子明;程偉;戴社教;白芝蘭;車向明;;兔VX2腎癌模型的建立及其影像學(xué)表現(xiàn)[J];第四軍醫(yī)大學(xué)學(xué)報;2006年07期

4 蘇暢;張惠中;李文海;林芳;梁曉華;江呂泉;程慶書;;兔VX2腫瘤的離體培養(yǎng)及有關(guān)生物學(xué)特性[J];第四軍醫(yī)大學(xué)學(xué)報;2006年09期

5 張克勤;張積仁;魏紅梅;;氬氦刀冷凍消融和射頻、微波熱凝固治療兔VX_2肝癌的對比研究[J];南方醫(yī)科大學(xué)學(xué)報;2007年09期

6 鄧曉軍,李振洪,黃一成,許景洪;原發(fā)性肝癌合并門靜脈癌栓的外科治療(附28例分析)[J];廣西醫(yī)科大學(xué)學(xué)報;2000年02期

7 張小玲;孟悛非;張朝暉;毛麗娟;;改良法兔VX2骨腫瘤模型的病理學(xué)與影像學(xué)觀察[J];中國骨腫瘤骨病;2007年02期

8 梁明輝;申寶忠;楊坡;;兔VX2肝癌模型的建立及綜合影像學(xué)的表現(xiàn)[J];齊齊哈爾醫(yī)學(xué)院學(xué)報;2008年04期

9 王曉東,任軍,楊仁杰,任秀昀,王曉東,張宏志,朱林中,于東升;VX2活細(xì)胞數(shù)與兔肝癌模型成功率及成瘤時間的關(guān)系[J];現(xiàn)代腫瘤醫(yī)學(xué);2005年05期

10 蔡宇紅;毛云英;;兔VX2腎移植癌模型傳代的改進(jìn)和螺旋CT評價[J];實用醫(yī)技雜志;2006年11期

，

本文編號：1873080