本文選題：苯代謝物 + 丙二醛　； 參考：《鄭州大學(xué)》2010年碩士論文

【摘要】： 苯是芳香族中結(jié)構(gòu)最簡(jiǎn)單的化合物,作為一種良好的有機(jī)溶劑被廣泛的應(yīng)用于工農(nóng)業(yè)生產(chǎn)中。長(zhǎng)期接觸苯可導(dǎo)致人類血液系統(tǒng)的損害,主要表現(xiàn)為血細(xì)胞減少癥、貧血、再生障礙性貧血和白血病等。但苯本身并不致癌,其致癌性是通過體內(nèi)代謝酶的代謝活化而產(chǎn)生的有毒代謝物質(zhì)所致。苯的毒性代謝產(chǎn)物氫醌和苯醌可在骨髓中產(chǎn)生活性氧簇(ROS),ROS可引發(fā)脂質(zhì)過氧化鏈?zhǔn)椒磻?yīng)產(chǎn)生羥基過氧化代謝產(chǎn)物,經(jīng)非酶性降解形成一些小分子的末端代謝產(chǎn)物,α,β-不飽和醛類化合物,如丙二醛(Malonaldehyde, MDA)和壬烯(4-hydroxy-2-nonenal, HNE)。 本文利用高效液相色譜、衍生化法等技術(shù)對(duì)苯作業(yè)工廠的工人和無苯作業(yè)工廠的工人的血漿樣品和尿樣中的丙二醛(Malonaldehyde, MDA)分析方法進(jìn)行了系統(tǒng)研究。研究?jī)?nèi)容包括以下三個(gè)方面： 1.通過對(duì)樣品前處理方法及色譜條件的優(yōu)化,建立了一種方便快捷的分離分析人血漿樣品中MDA的2-硫代巴比妥酸衍生化／高效液相色譜法,保留時(shí)間4.1 min,檢測(cè)限為0.043 nmol/mL。本實(shí)驗(yàn)采用了較低的衍生化反應(yīng)溫度(80℃),克服了以往因反應(yīng)溫度過高而引入非樣品所固有的MDA的缺點(diǎn)。該方法色譜峰型好,分離時(shí)間較短、靈敏度較高,大大提高了分析效率。 2.對(duì)樣品前處理方法和色譜分離條件的優(yōu)化選擇后,建立了一種方便快捷的分離分析人血漿樣品中MDA的2,4-二硝基苯肼衍生化／高效液相色譜法,保留時(shí)間25.9 min,檢測(cè)限為0.098 nmol/mL。本實(shí)驗(yàn)只需在室溫下便可快速完成衍生反應(yīng),此外,所需的pH條件也很溫和,該衍生化反應(yīng)對(duì)醛具有很強(qiáng)的專一性。 3.研究建立了一種可靠性強(qiáng)、靈敏度高的測(cè)定尿樣中MDA的2,4-二硝基苯肼衍生化／高效液相色譜法,保留時(shí)間8.9 min,檢測(cè)限為0.017 nmol/mL。該法前處理在37℃下進(jìn)行,條件溫和；采用“衍生化／萃取”一步法,操作簡(jiǎn)便,省時(shí)省力,適用于大批樣品的快速測(cè)定。
[Abstract]:Benzene is one of the simplest aromatic compounds and is widely used in industrial and agricultural production as a good organic solvent. Long-term exposure to benzene can lead to damage to the human blood system, mainly as hematopenia, anemia, aplastic anemia and leukemia. However, benzene itself is not carcinogenic. Its carcinogenicity is caused by toxic metabolites produced by metabolic activation of metabolic enzymes in vivo. Hydroquinone and benzoquinone, the toxic metabolites of benzene, can produce reactive oxygen species in bone marrow. Ros can induce lipid peroxidation chain reaction to produce hydroxyl peroxide metabolites. The end metabolites of some small molecules, 偽, 尾 -unsaturated aldehydes, such as malondialdehyde Malonaldehyde (MDAA) and nonene 4-hydroxy-2-nonenallic, HNEE, were formed by non-enzymatic degradation. In this paper, the methods for the determination of malonaldehyde (MDA) in plasma and urine samples of workers in benzene factory and in workers without benzene were systematically studied by means of high performance liquid chromatography (HPLC) and derivatization method. The research includes the following three aspects: 1. By optimizing the sample pretreatment method and chromatographic conditions, a convenient and rapid method for the separation and analysis of MDA in human plasma by 2-thiobarbituric acid derivatization / HPLC was established. The retention time was 4.1 min and the detection limit was 0.043 nmol 路mL ~ (-1). In this experiment, a relatively low temperature of 80 鈩，

本文編號(hào)：1862732