本文選題：苯代謝物 + 丙二醛��； 參考：《鄭州大學》2010年碩士論文

【摘要】： 苯是芳香族中結構最簡單的化合物,作為一種良好的有機溶劑被廣泛的應用于工農業(yè)生產中。長期接觸苯可導致人類血液系統(tǒng)的損害,主要表現(xiàn)為血細胞減少癥、貧血、再生障礙性貧血和白血病等。但苯本身并不致癌,其致癌性是通過體內代謝酶的代謝活化而產生的有毒代謝物質所致。苯的毒性代謝產物氫醌和苯醌可在骨髓中產生活性氧簇(ROS),ROS可引發(fā)脂質過氧化鏈式反應產生羥基過氧化代謝產物,經非酶性降解形成一些小分子的末端代謝產物,α,β-不飽和醛類化合物,如丙二醛(Malonaldehyde, MDA)和壬烯(4-hydroxy-2-nonenal, HNE)。 本文利用高效液相色譜、衍生化法等技術對苯作業(yè)工廠的工人和無苯作業(yè)工廠的工人的血漿樣品和尿樣中的丙二醛(Malonaldehyde, MDA)分析方法進行了系統(tǒng)研究。研究內容包括以下三個方面： 1.通過對樣品前處理方法及色譜條件的優(yōu)化,建立了一種方便快捷的分離分析人血漿樣品中MDA的2-硫代巴比妥酸衍生化／高效液相色譜法,保留時間4.1 min,檢測限為0.043 nmol/mL。本實驗采用了較低的衍生化反應溫度(80℃),克服了以往因反應溫度過高而引入非樣品所固有的MDA的缺點。該方法色譜峰型好,分離時間較短、靈敏度較高,大大提高了分析效率。 2.對樣品前處理方法和色譜分離條件的優(yōu)化選擇后,建立了一種方便快捷的分離分析人血漿樣品中MDA的2,4-二硝基苯肼衍生化／高效液相色譜法,保留時間25.9 min,檢測限為0.098 nmol/mL。本實驗只需在室溫下便可快速完成衍生反應,此外,所需的pH條件也很溫和,該衍生化反應對醛具有很強的專一性。 3.研究建立了一種可靠性強、靈敏度高的測定尿樣中MDA的2,4-二硝基苯肼衍生化／高效液相色譜法,保留時間8.9 min,檢測限為0.017 nmol/mL。該法前處理在37℃下進行,條件溫和；采用“衍生化／萃取”一步法,操作簡便,省時省力,適用于大批樣品的快速測定。
[Abstract]:Benzene is one of the simplest aromatic compounds and is widely used in industrial and agricultural production as a good organic solvent. Long-term exposure to benzene can lead to damage to the human blood system, mainly as hematopenia, anemia, aplastic anemia and leukemia. However, benzene itself is not carcinogenic. Its carcinogenicity is caused by toxic metabolites produced by metabolic activation of metabolic enzymes in vivo. Hydroquinone and benzoquinone, the toxic metabolites of benzene, can produce reactive oxygen species in bone marrow. Ros can induce lipid peroxidation chain reaction to produce hydroxyl peroxide metabolites. The end metabolites of some small molecules, 偽, 尾 -unsaturated aldehydes, such as malondialdehyde Malonaldehyde (MDAA) and nonene 4-hydroxy-2-nonenallic, HNEE, were formed by non-enzymatic degradation. In this paper, the methods for the determination of malonaldehyde (MDA) in plasma and urine samples of workers in benzene factory and in workers without benzene were systematically studied by means of high performance liquid chromatography (HPLC) and derivatization method. The research includes the following three aspects: 1. By optimizing the sample pretreatment method and chromatographic conditions, a convenient and rapid method for the separation and analysis of MDA in human plasma by 2-thiobarbituric acid derivatization / HPLC was established. The retention time was 4.1 min and the detection limit was 0.043 nmol 路mL ~ (-1). In this experiment, a relatively low temperature of 80 鈩，

本文編號：1862732