本文選題：心肌缺血再灌注損傷 + 缺氧誘導(dǎo)因子-��； 參考：《中外醫(yī)療》2009年22期

【摘要】：目的觀察缺氧誘導(dǎo)因子-1對(duì)大鼠心肌缺血再灌注損傷后急性炎癥應(yīng)答的影響。方法健康清潔級(jí)大鼠24只,隨機(jī)分為3組:二甲基乙二�；拾彼�(TMOG)組、對(duì)照組、假手術(shù)組,每組8只。TMOG組,在缺血與再灌注損傷模型建立前24h進(jìn)行腹腔內(nèi)注射20ug/gTMOG;對(duì)照組,在缺血與再灌注損傷模型建立前24h進(jìn)行腹腔內(nèi)注射生理鹽水0.5mL;除假手術(shù)組,其余2組通過結(jié)扎冠狀動(dòng)脈左前降支60min,然后松開180min建立心肌缺血再灌注損傷動(dòng)物模型;假手術(shù)組不結(jié)扎冠狀動(dòng)脈左前降支。3組大鼠均在實(shí)驗(yàn)終點(diǎn)采右心房血,分離血清,測(cè)定血清中的細(xì)胞間粘附分子-1(ICAM-1)、白細(xì)胞介素-8(IL-8)的表達(dá)水平;處死動(dòng)物取出心臟,測(cè)定大鼠心肌組織的缺氧誘導(dǎo)因子-1的mRNA表達(dá)。結(jié)果TMOG組缺氧誘導(dǎo)因子-1的mRNA表達(dá)比對(duì)照組明顯增多(P0.05);TMOG組血清中的ICAM-1及IL-8的表達(dá)水平明顯降低(P0.05)。結(jié)論缺氧誘導(dǎo)因子-1能通過抑制ICAM、IL-8的合成,減少中性粒細(xì)胞的浸潤,從而改善心肌缺血再灌注損傷,對(duì)心肌缺血再灌注損傷具有重要保護(hù)作用,為防治心肌缺血再灌注損傷提供了新的治療思路。
[Abstract]:Objective to observe the effect of hypoxia inducible factor-1 (HIF-1) on acute inflammatory response after myocardial ischemia reperfusion injury in rats. Methods Twenty-four healthy clean grade rats were randomly divided into three groups: dimethyl acetylglycine (TMOG) group, control group, sham-operated group, 8 rats in each group. 24 hours before the establishment of ischemia and reperfusion injury model, 20uggTMOG was injected intraperitoneally in the control group. 0.5 mL saline was injected intraperitoneally 24 hours before the establishment of ischemia and reperfusion injury model, the other two groups were ligated the left anterior descending coronary artery for 60 minutes, then released 180min to establish myocardial ischemia-reperfusion injury animal model. In the sham operation group, the right atrial blood was collected from the left anterior descending coronary artery at the end of the experiment, the serum was isolated, and the expression of intercellular adhesion molecule-1 (ICAM-1) and interleukin-8 (IL-8) in the serum were determined. The expression of hypoxia inducible factor-1 (mRNA) in rat myocardium was determined. Results the mRNA expression of hypoxia inducible factor-1 in TMOG group was significantly higher than that in control group. The expression of ICAM-1 and IL-8 in serum of TMOG group was significantly lower than that of control group. Conclusion Hypoxia-inducible factor 1 can reduce the infiltration of neutrophils by inhibiting the synthesis of IL-8 in ICAM, thus improving myocardial ischemia-reperfusion injury, and has an important protective effect on myocardial ischemia-reperfusion injury. It provides a new therapeutic idea for prevention and treatment of myocardial ischemia reperfusion injury.
【作者單位】： 福建中醫(yī)學(xué)院附屬人民醫(yī)院檢驗(yàn)科;福建醫(yī)科大學(xué);福建省立醫(yī)院心血管病重點(diǎn)實(shí)驗(yàn)室研究所;福建中醫(yī)學(xué)院附屬人民醫(yī)院ICU室;
【分類號(hào)】：R363

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本文編號(hào)：1848102