本文選題：抗氧化方法評價 + 相關性分析��； 參考：《陜西師范大學》2010年碩士論文

【摘要】： 體外抗氧化是抗氧化物質評價的重要方法,由于體外抗氧化檢測體系簡單,操作迅速,成本低,使得體外抗氧化評價方法在食品、藥品等抗氧化效應的評價方面使用日益廣泛,但是由于體外抗氧化檢測體系大多為純化學反應體系,不同檢測反應體系中所使用的自由基種類、自由基產生方法、自由基損傷的底物種類、損傷檢測原理等各不相同,導致不同的抗氧化檢測方法所獲得的結果相關性較差,不同抗氧化檢測方法對同一種抗氧化劑進行評價可以獲得不同的結果,甚至相互矛盾,利用不同抗氧化檢測方法對不同抗氧化劑進行評價,對抗氧化劑抗氧化能力強弱的判定往往差別較大,使得研究者難以給出確切的結論。 抗氧化效應的檢測目的在于篩選可以用于清除人體內自由基的抗氧化物質,用于疾病治療或者預防的目的,所以體外的抗氧化檢測方法需要盡可能吻合體內的狀況,才有可能真實的評價抗氧化物質的抗氧化效果,很多體外抗氧化方法采用的是純化學反應體系,所用的自由基種類和生理自由基不一致,檢測自由基損傷的分子種類與生物體內的損傷物質也不對應,使得體外檢測所得的抗氧化物質在體實驗往往效果不明顯。 我們以常見的四種抗氧化劑為試驗樣品,利用不同的抗氧化檢測方法對其抗氧化效果進行評估,根據(jù)評估的結果進行相關性分析,將相關性高的方法進行歸類,并依據(jù)其反應體系的特點對其進行初步分析評價,為研究者選擇合適的體外抗氧化方法提供科學依據(jù)。 通過對實驗結果的分析發(fā)現(xiàn)：第一,結晶紫法、2-脫氧-D-核糖法、TBA法,這位三種方法之間相關性較高,在抗氧化物質的能力檢測中,這三種方法應該一起選擇,得到的結果可能代表抗氧化劑對羥基自由基的清除能力或者脂質的保護能力。第二,肝臟線粒體脂質過氧化檢測分別與結晶紫法、TBA法、抑制蛋白質羰基化檢測、雙氧水誘導紅細胞溶血法有很好的結果相關性,是更適合生物體內情況的抗氧化檢測方法,可以用于檢測物質對生物膜系統(tǒng)的保護能力,或者對于脂質,蛋白質的保護能力。第三,清除DPPH自由基法、亞硝基R鹽鈷離子褪色法、鄰苯三酚自氧化法,這3種方法也是有較高的結果相關性的,可以用來檢測抗氧化劑對多數(shù)自由基的清除能力。
[Abstract]:Antioxidation in vitro is an important method for the evaluation of antioxidant substances. Because of its simplicity, rapid operation and low cost, in vitro antioxidant evaluation methods are increasingly used in the evaluation of antioxidant effects such as food, drugs and so on. However, the antioxidant detection systems in vitro are mostly pure chemical reaction systems. The kinds of free radicals used in different detection reaction systems, the methods of free radical generation, the types of free radical damage substrate, the principle of damage detection and so on are different. The results obtained by different antioxidant detection methods are poor, and different antioxidant detection methods can obtain different results, even contradictory results, when evaluating the same antioxidant. Using different antioxidant detection methods to evaluate different antioxidants, the determination of antioxidant capacity is often different, which makes it difficult for researchers to draw a definite conclusion. The purpose of antioxidant effect detection is to screen antioxidants that can be used to scavenge free radicals in human body, for disease treatment or prevention purposes, so in vitro antioxidant detection methods need to be as consistent as possible in vivo. It is possible to truly evaluate the antioxidant effect of antioxidants. Many methods of antioxidation in vitro use pure chemical reaction systems, and the kinds of free radicals used are inconsistent with physiological free radicals. The molecular types of free radical damage do not correspond to the damage substances in vivo, so the effect of antioxidants detected in vitro is not obvious. Four common antioxidants were used as test samples to evaluate the antioxidative effect by using different antioxidant detection methods. According to the results of the evaluation, the correlation analysis was carried out, and the highly correlated methods were classified. According to the characteristics of its reaction system, it was preliminarily analyzed and evaluated to provide scientific basis for the selection of suitable antioxidant methods in vitro. Through the analysis of the experimental results, it is found that: first, the crystal violet method and 2-deoxy--Dribose method TBA method, these three methods have a high correlation, in the ability of antioxidant substances, the three methods should be selected together. The results may indicate the ability of antioxidants to scavenge hydroxyl radicals or protect lipids. Second, the detection of liver mitochondrial lipid peroxidation has a good correlation with crystal violet method, inhibition of protein carbonylation, and hydrogen peroxide induced erythrocyte hemolysis. It is a more suitable antioxidant detection method in vivo. Can be used to test the protection of substances to the biofilm system, or to protect lipids, proteins. Third, scavenging DPPH free radical method, cobalt ion fading method of nitroso R salt and pyrogallol autooxidation method also have a high correlation with the results, which can be used to detect the scavenging ability of antioxidants to most free radicals.
【學位授予單位】：陜西師范大學
【學位級別】：碩士
【學位授予年份】：2010
【分類號】：R346

【引證文獻】

相關期刊論文 前2條

1 曹春蕾;韓美玲;崔寶凱;余海尤;;三種木層孔菌子實體不同溶劑提取物抗氧化活性的研究[J];菌物學報;2013年05期

2 田玉平;顧玲玲;;高效液相色譜法測定果汁飲料中的抗氧化劑[J];實驗室研究與探索;2012年10期

相關博士學位論文 前3條

1 侯麗麗;朝鮮淫羊藿有效成分的提取、分離關鍵技術及應用研究[D];吉林大學;2011年

2 劉春娟;鹿茸和鹿血活性多肽的研究[D];吉林大學;2012年

3 梁清;林蛙油流變學特性及應用研究[D];吉林大學;2012年

相關碩士學位論文 前4條

1 王燕;燕麥品種品質分析及油脂、多酚性質研究[D];中國農業(yè)科學院;2012年

2 楊穎;紅托竹蓀和辣乳菇化學成分及體外抗氧化活性研究[D];陜西師范大學;2012年

3 曹春蕾;桑木層孔菌生物學特性及多糖結構的研究[D];北京林業(yè)大學;2012年

4 杜遠東;老龍皮的藥效物質及質量標準研究[D];陜西中醫(yī)學院;2012年

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本文編號：1826110