本文選題：抗氧化方法評價(jià) + 相關(guān)性分析��； 參考：《陜西師范大學(xué)》2010年碩士論文

【摘要】： 體外抗氧化是抗氧化物質(zhì)評價(jià)的重要方法,由于體外抗氧化檢測體系簡單,操作迅速,成本低,使得體外抗氧化評價(jià)方法在食品、藥品等抗氧化效應(yīng)的評價(jià)方面使用日益廣泛,但是由于體外抗氧化檢測體系大多為純化學(xué)反應(yīng)體系,不同檢測反應(yīng)體系中所使用的自由基種類、自由基產(chǎn)生方法、自由基損傷的底物種類、損傷檢測原理等各不相同,導(dǎo)致不同的抗氧化檢測方法所獲得的結(jié)果相關(guān)性較差,不同抗氧化檢測方法對同一種抗氧化劑進(jìn)行評價(jià)可以獲得不同的結(jié)果,甚至相互矛盾,利用不同抗氧化檢測方法對不同抗氧化劑進(jìn)行評價(jià),對抗氧化劑抗氧化能力強(qiáng)弱的判定往往差別較大,使得研究者難以給出確切的結(jié)論。 抗氧化效應(yīng)的檢測目的在于篩選可以用于清除人體內(nèi)自由基的抗氧化物質(zhì),用于疾病治療或者預(yù)防的目的,所以體外的抗氧化檢測方法需要盡可能吻合體內(nèi)的狀況,才有可能真實(shí)的評價(jià)抗氧化物質(zhì)的抗氧化效果,很多體外抗氧化方法采用的是純化學(xué)反應(yīng)體系,所用的自由基種類和生理自由基不一致,檢測自由基損傷的分子種類與生物體內(nèi)的損傷物質(zhì)也不對應(yīng),使得體外檢測所得的抗氧化物質(zhì)在體實(shí)驗(yàn)往往效果不明顯。 我們以常見的四種抗氧化劑為試驗(yàn)樣品,利用不同的抗氧化檢測方法對其抗氧化效果進(jìn)行評估,根據(jù)評估的結(jié)果進(jìn)行相關(guān)性分析,將相關(guān)性高的方法進(jìn)行歸類,并依據(jù)其反應(yīng)體系的特點(diǎn)對其進(jìn)行初步分析評價(jià),為研究者選擇合適的體外抗氧化方法提供科學(xué)依據(jù)。 通過對實(shí)驗(yàn)結(jié)果的分析發(fā)現(xiàn)：第一,結(jié)晶紫法、2-脫氧-D-核糖法、TBA法,這位三種方法之間相關(guān)性較高,在抗氧化物質(zhì)的能力檢測中,這三種方法應(yīng)該一起選擇,得到的結(jié)果可能代表抗氧化劑對羥基自由基的清除能力或者脂質(zhì)的保護(hù)能力。第二,肝臟線粒體脂質(zhì)過氧化檢測分別與結(jié)晶紫法、TBA法、抑制蛋白質(zhì)羰基化檢測、雙氧水誘導(dǎo)紅細(xì)胞溶血法有很好的結(jié)果相關(guān)性,是更適合生物體內(nèi)情況的抗氧化檢測方法,可以用于檢測物質(zhì)對生物膜系統(tǒng)的保護(hù)能力,或者對于脂質(zhì),蛋白質(zhì)的保護(hù)能力。第三,清除DPPH自由基法、亞硝基R鹽鈷離子褪色法、鄰苯三酚自氧化法,這3種方法也是有較高的結(jié)果相關(guān)性的,可以用來檢測抗氧化劑對多數(shù)自由基的清除能力。
[Abstract]:Antioxidation in vitro is an important method for the evaluation of antioxidant substances. Because of its simplicity, rapid operation and low cost, in vitro antioxidant evaluation methods are increasingly used in the evaluation of antioxidant effects such as food, drugs and so on. However, the antioxidant detection systems in vitro are mostly pure chemical reaction systems. The kinds of free radicals used in different detection reaction systems, the methods of free radical generation, the types of free radical damage substrate, the principle of damage detection and so on are different. The results obtained by different antioxidant detection methods are poor, and different antioxidant detection methods can obtain different results, even contradictory results, when evaluating the same antioxidant. Using different antioxidant detection methods to evaluate different antioxidants, the determination of antioxidant capacity is often different, which makes it difficult for researchers to draw a definite conclusion. The purpose of antioxidant effect detection is to screen antioxidants that can be used to scavenge free radicals in human body, for disease treatment or prevention purposes, so in vitro antioxidant detection methods need to be as consistent as possible in vivo. It is possible to truly evaluate the antioxidant effect of antioxidants. Many methods of antioxidation in vitro use pure chemical reaction systems, and the kinds of free radicals used are inconsistent with physiological free radicals. The molecular types of free radical damage do not correspond to the damage substances in vivo, so the effect of antioxidants detected in vitro is not obvious. Four common antioxidants were used as test samples to evaluate the antioxidative effect by using different antioxidant detection methods. According to the results of the evaluation, the correlation analysis was carried out, and the highly correlated methods were classified. According to the characteristics of its reaction system, it was preliminarily analyzed and evaluated to provide scientific basis for the selection of suitable antioxidant methods in vitro. Through the analysis of the experimental results, it is found that: first, the crystal violet method and 2-deoxy--Dribose method TBA method, these three methods have a high correlation, in the ability of antioxidant substances, the three methods should be selected together. The results may indicate the ability of antioxidants to scavenge hydroxyl radicals or protect lipids. Second, the detection of liver mitochondrial lipid peroxidation has a good correlation with crystal violet method, inhibition of protein carbonylation, and hydrogen peroxide induced erythrocyte hemolysis. It is a more suitable antioxidant detection method in vivo. Can be used to test the protection of substances to the biofilm system, or to protect lipids, proteins. Third, scavenging DPPH free radical method, cobalt ion fading method of nitroso R salt and pyrogallol autooxidation method also have a high correlation with the results, which can be used to detect the scavenging ability of antioxidants to most free radicals.
【學(xué)位授予單位】：陜西師范大學(xué)
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2010
【分類號】：R346

【引證文獻(xiàn)】

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本文編號：1826110