發(fā)布時(shí)間：2018-04-30 09:03

  本文選題：骨髓基質(zhì)細(xì)胞 + 分化��； 參考：《山西醫(yī)科大學(xué)》2008年碩士論文

【摘要】： 目的:探討骨髓基質(zhì)細(xì)胞在傳代培養(yǎng)與誘導(dǎo)分化為神經(jīng)元過程中Hes1和Mash1基因的表達(dá)變化及其作用。方法:采用全骨髓培養(yǎng)法體外分離培養(yǎng)獲得骨髓基質(zhì)細(xì)胞,倒置顯微鏡下觀察其形態(tài)變化,應(yīng)用半定量RT-PCR技術(shù)分析Hes1和Mash1基因在傳代和誘導(dǎo)分化為神經(jīng)元過程中的動(dòng)態(tài)時(shí)序表達(dá)。結(jié)果:傳至3代以后的骨髓基質(zhì)細(xì)胞形態(tài)較為均一,誘導(dǎo)后細(xì)胞形態(tài)出現(xiàn)神經(jīng)元樣改變。在BMSCs傳代過程中,Hes1的表達(dá)逐漸下降;Mash1在傳至第5代時(shí)高表達(dá),隨后急劇下降。Hes1在P3的骨髓基質(zhì)細(xì)胞誘導(dǎo)后第1天表達(dá)下降;Mash1在P3的骨髓基質(zhì)細(xì)胞誘導(dǎo)后表達(dá)逐日增加。結(jié)論:Hes1和Mash1基因在骨髓基質(zhì)細(xì)胞傳代與誘導(dǎo)分化為神經(jīng)元過程中可能起重要作用。 目的:研究大鼠脊髓全橫斷損傷后,Wnt信號(hào)基因在不同時(shí)相脊髓的表達(dá)變化及對(duì)內(nèi)源性神經(jīng)干細(xì)胞的作用。 方法:成年健康Wistar大鼠30只,隨機(jī)分為假手術(shù)組(Sham組)和脊髓損傷組(SCI組),SCI組分為術(shù)后1、3、7、14、21 d組,每組5只。SCI組大鼠T11脊髓段做全橫斷損傷,Sham組僅行椎板切除術(shù),術(shù)后大鼠進(jìn)行BBB運(yùn)動(dòng)功能評(píng)分。采用半定量RT-PCR方法檢測(cè)脊髓損傷組織中Wnt信號(hào)基因的表達(dá)。 結(jié)果:SCI組所有動(dòng)物在術(shù)后均表現(xiàn)為雙后肢癱瘓,BBB運(yùn)動(dòng)功能評(píng)分明顯低于Sham組。SCI組在脊髓橫斷術(shù)后1、3、7 d Wnt3a表達(dá)均較Sham組明顯增加;尾側(cè)端Wnt3a較同時(shí)相頭側(cè)端的表達(dá)多,且在21 d又出現(xiàn)明顯的升高。在Sham組和SCI組均未檢測(cè)到Ngn1的表達(dá)。Hes1的表達(dá)在SCI后第1 d較Sham組有明顯的下降,第3 d出現(xiàn)升高,之后逐漸下降。結(jié)論:Wnt信號(hào)可能參與了脊髓損傷后再生修復(fù)的過程。
[Abstract]:Aim: to investigate the expression and role of Hes1 and Mash1 genes in bone marrow stromal cells (BMSCs) during subculture and differentiation into neurons. Methods: bone marrow stromal cells were isolated and cultured in vitro by whole bone marrow culture method. The morphologic changes of bone marrow stromal cells were observed under inverted microscope. Semi-quantitative RT-PCR technique was used to analyze the dynamic temporal expression of Hes1 and Mash1 genes during passage and differentiation into neurons. Results: the morphology of bone marrow stromal cells was uniform after 3 passage, and neuronal changes appeared after induction. During the passage of BMSCs, the expression of Hes1 decreased gradually and the expression of Mash1 increased gradually at the fifth passage, and then decreased sharply. The expression of Mash1 in P3 bone marrow stromal cells decreased on the first day after induction. The expression of Mash1 in P3 bone marrow stromal cells increased day after day. Conclusion the gene of 1: Hes1 and Mash1 may play an important role in the passage and differentiation of bone marrow stromal cells into neurons. Aim: to study the changes of Wnt signal gene expression in different phases of spinal cord and its effect on endogenous neural stem cells (NSCs) after total transection of spinal cord in rats. Methods: thirty adult healthy Wistar rats were randomly divided into sham-operated group (Sham group) and spinal cord injury group (sci group). BBB motor function score was performed in rats after operation. Semi-quantitative RT-PCR was used to detect the expression of Wnt signal gene in spinal cord injury tissues. Results the results showed that the motor function score of all the animals in group 1: sci was significantly lower than that in group Sham. The expression of Wnt3a was significantly higher in group 鈪，

本文編號(hào)：1823933