本文選題：動脈粥樣硬化 + 牙周炎　； 參考：《新疆醫(yī)科大學(xué)》2010年碩士論文

【摘要】：目的:建立兔牙周炎與動脈粥樣硬化結(jié)合模型,并對其進(jìn)行分析,探討牙周炎與動脈粥樣硬化間的聯(lián)系。方法:48只雄性新西蘭兔隨機分為4組:對照組(A組),牙周炎組(B組),動脈粥樣硬化組(C組),牙周炎+動脈粥樣硬化(D組)。各組分別按照實驗設(shè)計進(jìn)行相應(yīng)處理,檢測牙周指標(biāo)、血脂指標(biāo)及血清中高敏C反應(yīng)蛋白、基質(zhì)金屬蛋白酶9的含量,12周末處死動物,X線觀察實驗牙牙槽骨吸收情況,取升主動脈行HE及Masson染色,進(jìn)行斑塊面積及穩(wěn)定性評估,免疫組化染色檢測斑塊內(nèi)基質(zhì)金屬蛋白酶9、單核細(xì)胞趨化蛋白-1含量。結(jié)果:B、D組探診深度和齦溝出血指數(shù)均高于A、C組(P0.01),X線見實驗牙牙槽骨吸收明顯,C、D組血脂指標(biāo)均高于A組及B組(P0.01),病理見C、D組動脈粥樣硬化形成,同時D組病變較C組更加嚴(yán)重,斑塊更加不穩(wěn)定。血清高敏C反應(yīng)蛋白、基質(zhì)金屬蛋白酶9含量D組最高,C、B、A組依次減少。免疫組化染色見D組斑塊內(nèi)基質(zhì)金屬蛋白酶9、單核細(xì)胞趨化蛋白-1表達(dá)多于C組(P0.01),同時可見B組內(nèi)膜少許表達(dá)。結(jié)論:牙周炎可以加重動脈粥樣硬化的病變程度并使斑塊更加不穩(wěn)定,這種作用可能與牙周炎時體內(nèi)更加嚴(yán)重的炎癥狀態(tài),及斑塊局部基質(zhì)金屬蛋白酶9、單核細(xì)胞趨化蛋白-1等細(xì)胞因子表達(dá)水平的升高有關(guān)。
[Abstract]:Aim: to establish a rabbit model of periodontitis and atherosclerosis and analyze the relationship between periodontitis and atherosclerosis. Methods Forty-eight male New Zealand rabbits were randomly divided into four groups: control group A, periodontitis group B, atherosclerosis group C, periodontitis atherosclerosis group D. The periodontal index, blood lipid index, Gao Min C-reactive protein and matrix metalloproteinase-9 in serum were detected in each group according to the experimental design. After 12 weeks, animals were killed to observe the alveolar bone resorption. The ascending aorta was stained with HE and Masson, and the plaque area and stability were evaluated. The contents of matrix metalloproteinase 9 and monocyte chemoattractant protein-1 were detected by immunohistochemical staining. Results the depth of probing and the bleeding index of gingival sulcus in group D were higher than those in group A (P 0.01). The blood lipid indexes in group C were higher than those in group A and group B, and the atherosclerosis was found in group C, and the lesion in group D was more serious than that in group C. Plaque is more unstable. Serum Gao Min C-reactive protein and matrix metalloproteinase 9 content in group D were decreased in turn. Immunohistochemical staining showed that matrix metalloproteinase 9 and monocyte chemoattractant protein-1 were more expressed in plaque of group D than those in group C (P 0.01) and a little in group B. Conclusion: periodontitis can aggravate the severity of atherosclerosis and make the plaque more unstable, which may be related to the more severe inflammation in the body during periodontitis. The expression of matrix metalloproteinase 9 and monocyte chemoattractant protein-1 were related to the increase of matrix metalloproteinase 9 and monocyte chemoattractant protein-1.
【學(xué)位授予單位】：新疆醫(yī)科大學(xué)
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2010
【分類號】：R-332;R781.4;R543.5

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