本文選題：鐵皮石斛多糖 + 冠心病�。� 參考：《中國實驗方劑學雜志》2015年21期

【摘要】：目的:觀察鐵皮石斛多糖對冠心病模型家兔心功能、心肌收縮能力的影響,并探討其相關作用機制。方法:30只健康家兔隨機分為對照組、模型組、鐵皮石斛多糖低、中、高劑量組5組,每組6只。采用左冠狀動脈放置縮窄環(huán)方法,制備冠心病模型。低、中、高劑量組鐵皮石斛多糖按100,200,400 mg·kg-1ig給予,每天1次,連續(xù)21 d。對照組、模型組ig給予等體積生理鹽水。記錄左室收縮壓(LVSP),左室舒張末壓(LVEDP),左室壓力變化最大上升和下降速率(±dp/dtmax),動脈收縮壓(BPs)和動脈舒張壓(BPd),心室質量指數(shù),RT-PCR法檢測心肌細胞α-肌球蛋白(α-MHC)mRNA,β-肌球蛋白(β-MHC)mRNA表達,免疫組化檢測肌動蛋白(actin)表達。結果:與對照組比較,模型組LVEDP,LVWI,RVWI均升高(P0.01,P0.05),而LVSP,±dp/dtmax,BPs均降低(P0.01,P0.05),α-MHC mRNA表達顯著下降(P0.01),β-MHC mRNA升高(P0.05),模型組actin蛋白表達呈陽性的心肌細胞較多。與模型組比較,鐵皮石斛多糖低、中劑量組LVEDP降低(P0.01,P0.05),而LVSP,±dp/dtmax,BPs均升高(P0.01,P0.05),α-MHC mRNA表達顯著升高(P0.01),β-MHC mRNA表達下降(P0.05),各劑量組actin蛋白表達呈陽性的心肌細胞較少。結論:鐵皮石斛多糖一定程度上恢復心肌舒縮能力、改善心功能。
[Abstract]:Aim: to investigate the effects of Dendrobium candidum polysaccharides on cardiac function and myocardial contractility in rabbits with coronary heart disease (CHD). Methods 30 healthy rabbits were randomly divided into control group, model group, Dendrobium candidum polysaccharide low, medium and high dose groups, with 6 in each group. Coronary artery disease (CHD) model was established by placing constrictive ring in left coronary artery. Dendrobium candidum polysaccharides in low, medium and high dose groups were given 100200400 mg kg-1ig once a day for 21 days. The control group and the model group were given the same volume of saline intravenously. Left ventricular systolic pressure (LVSPN), left ventricular end-diastolic pressure (LVEDPN), left ventricular pressure maximum rise and fall rate (鹵DP / dtmax) and arterial diastolic pressure (DBP) were recorded. The expression of 偽 -MHCmRNAs (尾 -MHCmRNAs, 尾 -MHCN mRNA) in cardiomyocytes was detected by RT-PCR. The expression of actin was detected by immunohistochemistry. Results: compared with the control group, the RVWI of LVEDPN LVWIWI in the model group was significantly higher than that in the control group, while the expression of actin protein in the model group was significantly lower than that in the control group. The expression of 偽 -MHC mRNA was significantly decreased (P 0.01) and the expression of 尾 -MHC mRNA was higher in the model group than in the control group. The positive expression of actin protein in the model group was higher than that in the model group. The expression of actin protein in the model group was more than that in the model group. Compared with the model group, Dendrobium candidum polysaccharide was lower in the middle dose group than that in the middle dose group. However, the LVEDP in the middle dose group was lower than that in the control group, while that in the 鹵DP / dtmaxus BP was higher than that in the model group. The expression of 偽 -MHC mRNA was significantly higher than that in the model group, and the expression of 尾 -MHC mRNA was decreased in the cardiac myocytes with positive expression of actin protein. Conclusion: Dendrobium candidum polysaccharides can improve cardiac function and improve cardiac function.
【作者單位】： 右江民族醫(yī)學院;惠州衛(wèi)生職業(yè)技術學院;
【基金】：廣西科學研究與技術開發(fā)計劃項目(桂科攻1347003-4) 廣西教育廳科研立項項目(201106LX443)
【分類號】：R285.5;R-332

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