本文選題：多巴胺 + 鈉氫交換體��； 參考：《吉林大學》2010年碩士論文

【摘要】： 小劑量多巴胺具有利鈉、利尿作用,并被廣泛用于各種相關(guān)病癥的治療,但近年來多巴胺的作用逐漸受到質(zhì)疑。由于腎臟局部可產(chǎn)生多巴胺,本實驗給予低劑量左旋多巴,在無外周血管作用同時,研究其在腎臟局部對水鹽代謝的調(diào)節(jié)作用。本實驗通過RT-PCR?及?Western?blotting等方法分析了近曲小管NHE3和鈉鉀?ATP酶的表達及定位,結(jié)果發(fā)現(xiàn):低劑量左旋多巴持續(xù)灌注并不影響血壓及腎小球濾過率,但腎臟排水排鈉增加。腎臟局部多巴胺水平增加使近曲小管內(nèi)皮細胞?NHE3?表達降低,鈉鉀?ATP?酶水平未發(fā)生變化。由此推測多巴胺通過瞬時調(diào)節(jié)?NHE3快迅內(nèi)化,從而減少鈉的重吸收。綜合各種檢測結(jié)果,本研究認為腎臟局部多巴胺可通過誘導近曲小管細胞膜NHE3向胞內(nèi)轉(zhuǎn)移發(fā)揮利鈉作用。多巴胺的利鈉作用主要通過瞬時調(diào)節(jié)NHE3穿梭來實現(xiàn)的而不是通過長調(diào)節(jié)來實現(xiàn)的。近曲小管上皮細胞鈉的重吸收?NHE3?起到限速作用,鈉鉀?ATP?酶只是鈉重吸收的附助條件。
[Abstract]:Low dose dopamine has natriuretic and diuretic effects, and has been widely used in the treatment of various related diseases, but the role of dopamine has gradually been questioned in recent years.Since dopamine can be produced locally in the kidney, low dose levodopa was given to study the regulation of water and salt metabolism in the kidney without peripheral vascular effect.In this experiment, RT-PCR?The expression and localization of NHE3 and Na + K + ATPase in proximal convoluted tubules were analyzed by Western blotting. The results showed that low dose levodopa infusion did not affect blood pressure and glomerular filtration rate, but increased renal sodium drainage.The increase of local dopamine level in the kidney causes NHE3 to be expressed in the endothelial cells of proximal convoluted tubules.Decreased expression, Na + K ATP?The enzyme level did not change.It is inferred that dopamine can reduce sodium reabsorption by transient regulation of NHE3 internalization.In this study, we concluded that local dopamine could induce the transfer of NHE3 to the cell membrane of proximal convoluted tubules.The effect of dopamine sodium is mainly achieved by transient regulation of NHE3 shuttle rather than long regulation.Sodium reabsorption in epithelial cells of proximal convoluted tubulesPlay a role in limiting the speed, sodium potassium ATP?Enzyme is only a secondary condition for sodium reabsorption.
【學位授予單位】：吉林大學
【學位級別】：碩士
【學位授予年份】：2010
【分類號】：R33

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相關(guān)期刊論文 前2條

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本文編號：1753043