發(fā)布時(shí)間：2018-04-14 21:40

  本文選題：曲霉 + 系統(tǒng)發(fā)育　； 參考：《吉林大學(xué)》2009年博士論文

【摘要】： 曲霉與人類的日常生活具有密切關(guān)系。有些曲霉在人類生產(chǎn)實(shí)踐中已被安全應(yīng)用了近千年,有些曲霉能產(chǎn)生真菌毒素,對農(nóng)業(yè)生產(chǎn)及食品安全造成危害,還有一些曲霉能引起人及動(dòng)物的侵襲性感染。為了闡明曲霉不同菌種間的系統(tǒng)發(fā)育關(guān)系及分類地位,澄清不同曲霉之間的親緣關(guān)系與進(jìn)化路徑,本研究對具有重要經(jīng)濟(jì)應(yīng)用價(jià)值和臨床上常見的共26個(gè)種的曲霉進(jìn)行了系統(tǒng)發(fā)育分析。通過對曲霉四種持家基因GAPDH基因、β-tubulin基因、MTO1基因和HSP70基因的編碼區(qū)部分序列設(shè)計(jì)兼并引物,對這些基因進(jìn)行了擴(kuò)增測序,并提交GenBank。在此基礎(chǔ)上對各基因的堿基組成、堿基替換特征及各基因序列間的P距離進(jìn)行了分析。并采用鄰接法(NJ)、非加權(quán)分組平均法(UPGMA)法、最大似然法(ML)及最大簡約法(MP)對各基因及四種基因的聯(lián)合序列構(gòu)建了系統(tǒng)發(fā)育樹。通過分析,對這26種曲霉的系統(tǒng)發(fā)育關(guān)系及分類地位進(jìn)行了闡述,并結(jié)合形態(tài)學(xué)特征對一些菌株進(jìn)行了重新的鑒定和分類。 在以上相關(guān)工作基礎(chǔ)上,通過對曲霉四種基因測得序列的比對分析,針對病原性煙曲霉、構(gòu)巢曲霉及黑曲霉的特異位點(diǎn)設(shè)計(jì)引物及Taqman探針,建立了應(yīng)用Real-time PCR技術(shù)快速檢測這三種病原性曲霉的方法。
[Abstract]:Aspergillus has a close relationship with human daily life.Some Aspergillus have been safely used in human production for nearly a thousand years, some Aspergillus can produce mycotoxins, which can cause harm to agricultural production and food safety, and others can cause invasive infection in humans and animals.In order to elucidate the phylogenetic relationship and taxonomic status of Aspergillus sp., the phylogenetic relationship and evolutionary pathway between Aspergillus sp.The phylogenetic analysis of 26 species of Aspergillus sp.The sequence of GAPDH gene, 尾 -tubulin gene, MTO1 gene and HSP70 gene of Aspergillus sp., were amplified and sequenced.On this basis, the base composition, base substitution characteristics and P distance of each gene sequence were analyzed.The phylogenetic tree was constructed by using the adjacent method, the unweighted group average method, the maximum likelihood method (MLL) and the maximum minimization method (MPP) for the combined sequences of each gene and four genes.The phylogenetic relationship and taxonomic status of 26 Aspergillus species were analyzed, and some strains were reidentified and classified according to morphological characteristics.Based on the above work, primer and Taqman probe were designed for the specific sites of Aspergillus fumigatus, Aspergillus Niger and Aspergillus Niger.A method for rapid detection of these three pathogenic aspergillus by Real-time PCR was established.
【學(xué)位授予單位】：吉林大學(xué)
【學(xué)位級別】：博士
【學(xué)位授予年份】：2009
【分類號】：R379;R450

【引證文獻(xiàn)】

相關(guān)碩士學(xué)位論文 前1條

1 李亞紅;豫、鄂、渝三省（市）小麥白粉菌群體遺傳結(jié)構(gòu)研究[D];河南農(nóng)業(yè)大學(xué);2011年

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本文編號：1751116