本文選題：孕鼠 + 強(qiáng)迫游泳試驗(yàn)(FST)��； 參考：《中國醫(yī)科大學(xué)》2008年碩士論文

【摘要】： 目的 觀察孕鼠在強(qiáng)迫游泳實(shí)驗(yàn)(FST)中的不動(dòng)時(shí)間,了解孕鼠是否具有更明顯的行為絕望表現(xiàn)(即停止掙扎、不動(dòng)時(shí)間延長);同時(shí)觀察反復(fù)強(qiáng)迫游泳后孕鼠的行為絕望程度(不動(dòng)時(shí)間長短)是否加重; 半定量測定強(qiáng)迫游泳后小鼠大腦皮質(zhì)雌激素α受體(ER_α)的表達(dá)水平,比較孕鼠和非孕鼠之間的差異; 簡單分析孕鼠在FST中的不動(dòng)時(shí)間與中樞ER_α表達(dá)水平之間的數(shù)量關(guān)系;初步探討小鼠FST的行為絕望程度(不動(dòng)時(shí)間長短)與中樞ER_α表達(dá)水平之間是否直接相關(guān)。 材料及方法 同批次雌昆明鼠,來源于中國醫(yī)科大學(xué)動(dòng)物實(shí)驗(yàn)中心。隨機(jī)分為受孕組(合籠受孕)和對照組,共獲得31只孕鼠和27只對照鼠;其中有3只孕鼠早產(chǎn),未進(jìn)行第6次FST和免疫組化檢測。 孕鼠受精后12天,開始進(jìn)行FST,隨機(jī)抽取非孕鼠作為對照;一共6次FST,記錄第2次和第6次的不動(dòng)時(shí)間。 完成6次FST后,取大腦組織,共有28只孕鼠和13只對照鼠標(biāo)本;以海馬為中心縱切,石蠟包埋,切片行免疫組化鏈霉菌抗生物素-過氧化物酶(SP)法半定量檢測大腦皮質(zhì)ER_α。 統(tǒng)計(jì)學(xué)分析采用SPSS13.0軟件;計(jì)量資料不滿足正態(tài)分布和方差齊性,采取Mann-Whitney U檢驗(yàn);滿足總體正態(tài)和樣本方差齊性,采取兩獨(dú)立樣本t檢驗(yàn);兩變量間關(guān)系采用Pearson直線相關(guān)分析。 結(jié)果 孕鼠在第2次和第6次FST中不動(dòng)時(shí)間均長于非孕鼠,P分別為0.011和0.016,差異有統(tǒng)計(jì)學(xué)意義;孕鼠第6次FST比第2次FST不動(dòng)時(shí)間長,P為0.016,差異有統(tǒng)計(jì)學(xué)意義。 強(qiáng)迫游泳后孕鼠和對照鼠大腦皮質(zhì)均有ER_α表達(dá),但孕鼠中樞ER_α組化評分高于非孕鼠,P為0.008,差異有統(tǒng)計(jì)學(xué)意義。 比較第6次FST不動(dòng)時(shí)間t＞180秒的孕鼠和t＜180秒的孕鼠之間大腦皮質(zhì)ER_α組化評分差異,P為0.128,無統(tǒng)計(jì)學(xué)意義。 小鼠兩次FST的不動(dòng)時(shí)間分別與中樞ER_α組化評分做散點(diǎn)圖,均無明顯直線相關(guān)趨勢,R值為0.041和0.082,P值0.800和0.607,回歸系數(shù)均無顯著意義,不能認(rèn)為有直線關(guān)系。 結(jié)論 孕鼠在FST中不動(dòng)時(shí)間長于非孕鼠,行為絕望狀態(tài)更明顯,具有抑郁傾向;經(jīng)過6次強(qiáng)迫游泳后孕鼠在FST中不動(dòng)時(shí)間延長,可考慮作為一種產(chǎn)前抑郁癥的模型。 強(qiáng)迫游泳后孕鼠中樞ER_α表達(dá)水平明顯高于非孕鼠,它可能與產(chǎn)前抑郁癥的發(fā)生有關(guān)。 孕鼠在FST中不動(dòng)時(shí)間的長短與中樞ER_α表達(dá)水平之間尚未發(fā)現(xiàn)有數(shù)量上的聯(lián)系;小鼠在FST不動(dòng)時(shí)間與中樞ER_α水平之間尚未發(fā)現(xiàn)有直線關(guān)系,暫不能認(rèn)為二者直接相關(guān)。
[Abstract]:PurposeTo observe the immobility time of pregnant rats in forced swimming experiment (FSTs), to find out if pregnant rats have more obvious behavioral despair (that is, stop struggling).The time of immobility was prolonged and the degree of behavioral despair (length of immobility) of pregnant rats after repeated forced swimming was observed.The expression level of estrogen 偽 receptor ER偽 in cerebral cortex of mice after forced swimming was measured semi-quantitatively to compare the difference between pregnant and non-pregnant mice.The quantitative relationship between the immobility time of pregnant rats in FST and the expression level of central ER- 偽 was analyzed, and the relationship between the degree of behavioral despair (immobility time) of FST and the expression level of central ER- 偽 in mice was preliminarily investigated.Materials and methodsThe same batch of female Kunming mice came from the Animal experiment Center of China Medical University.31 pregnant rats and 27 control mice were randomly divided into the pregnant group and the control group, 3 of them were preterm, and the sixth FST and immunohistochemistry were not performed.After 12 days of fertilization, FSTs were performed and non-pregnant mice were randomly selected as control, and the second and sixth immobility times were recorded for 6 FSTs.After 6 times of FST, 28 pregnant rats and 13 control mice were taken from the brain tissue, the hippocampus was cut longitudinally, embedded in paraffin, and the ER偽 in the cerebral cortex was detected by immunohistochemical streptomyces biotin peroxidase (SPSP) method.The statistical analysis adopts SPSS13.0 software; the measurement data do not satisfy the normal distribution and homogeneity of variance, the Mann-Whitney U test is used; the total normal distribution and sample variance homogeneity are satisfied, and two independent samples t test are adopted; the relationship between the two variables is analyzed by Pearson linear correlation analysis.ResultThe immobility time of pregnant rats in the second and sixth FST was 0.011 and 0.016, respectively, the difference was statistically significant, and the FST of the sixth pregnant mice was 0.016 longer than that of the second FST (P = 0.016).There was ER偽 expression in the cerebral cortex of pregnant rats and control rats after forced swimming, but the ER _ 偽 histochemical score of pregnant rats was higher than that of non-pregnant rats (P = 0.008), and the difference was statistically significant.The difference of ER偽 histochemical score between pregnant rats with FST immobility time t > 180 seconds and pregnant rats with t < 180 seconds was 0.128, which had no statistical significance.The fixed time of FST in mice and the central ER- 偽 histochemical score were divided into scattered plot. There was no obvious linear correlation R value of 0.041 and 0.082% P value 0.800 and 0.607, respectively. The regression coefficient was not significant and could not be regarded as a linear relationship.ConclusionThe immobility time of pregnant mice in FST was longer than that of non-pregnant mice, the behavior of desperate mice was more obvious, and the pregnant mice had depression tendency. After 6 times of forced swimming, the pregnant rats' immobility time in FST was prolonged, so the pregnant mice could be considered as a model of prenatal depression.The level of ER偽 expression in pregnant rats after forced swimming was significantly higher than that in non-pregnant rats, which may be related to the occurrence of prenatal depression.There was no quantitative correlation between the length of immobility time in FST and the expression level of central ER偽 in pregnant mice, but there was no linear relationship between FST immobility time and central ER偽 level in mice, so it was not possible to think that the two were directly related.
【學(xué)位授予單位】：中國醫(yī)科大學(xué)
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2008
【分類號】：R714.7;R395

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