本文選題：免疫耐受 + 泡球蚴感染�。� 參考：《新疆醫(yī)科大學(xué)》2009年碩士論文

【摘要】： 目的:建立C57BL/6小鼠對(duì)BALB/c小鼠的尾部皮膚移植模型,觀(guān)察健康小鼠及感染泡球蚴1月及3月移植皮片存活情況,探討感染泡球蚴的小鼠皮膚移植后,其免疫系統(tǒng)的變化對(duì)移植物的影響。方法:采用小鼠尾部皮膚移植建立小鼠皮膚移植模型。實(shí)驗(yàn)選用64只健康的C57BL/6雄性小鼠,80只健康BALB/c雄性小鼠,隨機(jī)選取其中48只接種包蟲(chóng)。實(shí)驗(yàn)分為4組,每組各16對(duì):A組.空白對(duì)照組(非同系移植不予以任何干預(yù)):健康C57BL/6小鼠→健康BALB/c小鼠; B組.實(shí)驗(yàn)組1:健康C57BL/6小鼠→BALB/c小鼠(感染泡球蚴后1個(gè)月后); C組.藥物對(duì)照組[非同系移植加藥物干預(yù):新型免疫抑制劑西羅莫司(雷鉑霉素)]:健康C57BL/6小鼠→健康BALB/c小鼠,皮膚移植術(shù)后五天每日給予西羅莫司1.5mg/kg.d干預(yù);D組.實(shí)驗(yàn)組2:健康C57BL/6小鼠→BALB/c小鼠(感染泡球蚴后3個(gè)月后)。術(shù)后觀(guān)察BALB/c小鼠尾部移植皮膚生長(zhǎng)情況,有無(wú)蒼白,干癟,結(jié)痂,毛發(fā)脫落等情況,出現(xiàn)上述情況視為移植皮片死亡,若出現(xiàn)包扎脫落等情況視為技術(shù)失敗,技術(shù)失敗小鼠不納入實(shí)驗(yàn)中并及時(shí)補(bǔ)足小鼠只數(shù)。每組留8只用于觀(guān)察BALB/c小鼠術(shù)后尾部移植皮膚生存期,每組于術(shù)后3、5、7、9天四個(gè)時(shí)間點(diǎn)各取兩只小鼠尾部移植皮片,用于觀(guān)察小鼠尾部移植皮膚病理變化情況。結(jié)果:A組、B組、C組、D組移植皮膚存活時(shí)間平均分為6.0±0.76、7.5±0.93、13.25±1.04、11.5±0.93天。C、D組移植皮膚存活時(shí)間較A、B組延長(zhǎng),差異有統(tǒng)計(jì)學(xué)意義(p0.01),組織病理學(xué)檢查:A、B組移植皮片均在術(shù)后一周左右發(fā)生明顯的逐漸加重的急性免疫排斥反應(yīng), C、D組移植皮片的免疫排斥反應(yīng)較A、B組輕。結(jié)論:小鼠感染泡狀棘球蚴后有利于同種異體移植皮片的存活,可以延長(zhǎng)移植皮片的生存時(shí)間。該模型為研究感染泡球的免疫實(shí)驗(yàn)研究提供了良好的實(shí)驗(yàn)平臺(tái),為進(jìn)一步研究泡球蚴感染后機(jī)體免疫狀態(tài)的變化提供了基礎(chǔ)。
[Abstract]:Objective: to establish the tail skin transplantation model of BALB/c mice in C57BL/6 mice and to observe the survival rate of transplanted skin grafts in healthy mice and infected alveolar echinococci for one month and three months, and to explore the skin transplantation of mice infected with alveolar echinococci.The effects of changes in its immune system on grafts.Methods: mouse skin transplantation model was established by tail skin transplantation.Sixty-four healthy C57BL/6 male mice and 80 healthy BALB/c male mice were selected and 48 of them were randomly inoculated with hydatid worms.The experiment was divided into 4 groups, 16 pairs in each group.Blank control group (non-homologous transplantation without any intervention: healthy C57BL/6 mice healthy BALB/c mice; group B).Experimental group 1: healthy C57BL/6 mice BALB/c mice (1 month after infection with alveolar hydatid); C group.Drug control group [non-homologous transplantation plus drug intervention: a new immunosuppressant sirolimus (rifamycin)]: healthy C57BL/6 mice were treated with sirolimus 1.5mg/kg.d for 5 days after skin transplantation.Group 2: healthy C57BL/6 mice BALB/c mice (3 months after infection with alveolar hydatid).After operation, the skin growth of BALB/c mice was observed, whether the skin was pale, dry, scabbed, hair shed and so on. The above condition was regarded as the death of skin grafts, and if the cases of bandaging and shedding were regarded as technical failure,The technique failed mice were not included in the experiment and the number of mice was replenished in time.Eight skin grafts were used in each group to observe the survival time of BALB/c mice after operation. Two mice skin grafts were taken from each group at four time points of 79-day after operation to observe the pathological changes of the skin grafted from the tail of the mice.Results the average survival time of skin graft in group C and D was 6.0 鹵0.76 鹵0.93 days. The survival time of skin grafts in group C was longer than that in group A (13.25 鹵1.04U 11.5 鹵0.93 days).The histopathological examination showed that the acute immune rejection of grafts in group 1 was significantly worse than that in group Agna B, and that in group C D was less than that in group Agna B (P < 0 01), and that in group C was significantly worse than that in group A (P < 0 01).Conclusion: infection with Echinococcus alveolaris in mice is beneficial to the survival of allograft skin grafts and can prolong the survival time of skin grafts.The model provides a good experimental platform for the study of immunological study of infected alveolar spheres, and provides a basis for further study on the changes of immune state after infection of alveolar echinococci.
【學(xué)位授予單位】：新疆醫(yī)科大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2009
【分類(lèi)號(hào)】：R392

【引證文獻(xiàn)】

相關(guān)碩士學(xué)位論文 前1條

1 李紅衛(wèi);棘球蚴自然抗原和卡介苗對(duì)Th1/Th2轉(zhuǎn)錄因子和細(xì)胞因子的影響[D];寧夏醫(yī)科大學(xué);2011年

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