發(fā)布時間：2018-04-03 17:01

  本文選題：人骨髓間充質(zhì)干細胞　切入點：MicroRNA--p　出處：《中國病理生理雜志》2015年02期

【摘要】：目的:研究年齡相關(guān)microRNA-708-5p(miR-708-5p)對人骨髓間充質(zhì)干細胞(h MSCs)遷移能力的調(diào)控作用。方法:通過microRNA芯片和real-time PCR檢測供體年齡對h MSCs中miR-708-5p表達的影響;通過轉(zhuǎn)染miR-708-5p模擬物或抑制物,過表達或抑制miR-708-5p表達;通過細胞劃痕及Transwell實驗檢測h MSCs遷移能力。通過siRNA研究miR-708-5p靶基因跨膜蛋白88(TMEM88)對h MSCs中β鏈球蛋白(β-catenin)及h MSCs遷移功能的影響。結(jié)果:隨供體年齡增加,h MSCs中miR-708-5p的表達下降。過表達miR-708-5p可促進h MSCs遷移。相反,抑制miR-708-5p的表達可減少h MSCs遷移。隨供體年齡增加,TMEM88表達增加,而β-catenin表達下降,直接抑制TMEM88的表達可促進β-catenin表達,促進h MSCs遷移。同時抑制miR-708-5p和TMEM88,使miR-708-5p失去對h MSCs的調(diào)控作用。結(jié)論:miR-708-5p可通過抑制TMEM88表達,上調(diào)β-catenin,從而活化Wnt/β-catenin信號通路,促進h MSCs遷移。
[Abstract]:Aim: to investigate the effect of age-related microRNA-708-5pmmiR-708-5p on the migration of human bone marrow mesenchymal stem cells (MSCs).Methods: the effect of donor age on the expression of miR-708-5p in h MSCs was detected by microRNA microarray and real-time PCR, the expression of miR-708-5p was over-expressed or inhibited by transfection of miR-708-5p mimics or inhibitors, and the migration ability of h MSCs was detected by cell scratch and Transwell experiments.The effects of miR-708-5p target gene transmembrane protein 88 (TMEM88) on 尾 -catenin (尾 -catenin) and the migration function of h MSCs in h MSCs were studied by siRNA.Results: the expression of miR-708-5p decreased with the age of donor.Overexpression of miR-708-5p can promote the migration of h MSCs.On the contrary, inhibiting the expression of miR-708-5p reduced the migration of h MSCs.The expression of 尾 -catenin increased with the increase of donor age, while the expression of 尾 -catenin decreased. Directly inhibiting the expression of TMEM88 could promote the expression of 尾 -catenin and promote the migration of h MSCs.Both miR-708-5p and TMEM88 were inhibited, which caused miR-708-5p to lose its regulatory effect on h MSCs.Conclusion: 1 miR-708-5p can activate Wnt/ 尾 -catenin signaling pathway and promote h MSCs migration by inhibiting the expression of TMEM88 and up-regulating 尾 -catenin.
【作者單位】： 廣州醫(yī)科大學附屬第二醫(yī)院廣州心血管疾病研究所;廣州醫(yī)科大學附屬第二醫(yī)院心內(nèi)科;廣州醫(yī)科大學附屬第二醫(yī)院腫瘤科;
【基金】：國家自然科學基金青年基金資助項目(No.81401156) 廣州市醫(yī)藥衛(wèi)生科技項目(No.20141A011088)
【分類號】：R329.2
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本文編號：1706101