本文選題：氣管上皮種植　切入點(diǎn)：模型　出處：《蘭州大學(xué)》2009年碩士論文　論文類型：學(xué)位論文

【摘要】： 目的通過(guò)同種異體大鼠氣管的異位移植,建立氣道上皮再生的模型。方法實(shí)驗(yàn)組(n=20)取Wistar大鼠氣管經(jīng)胰蛋白酶冷消化制成去上皮化氣管作為供體,灌入含分化成熟的氣管上皮細(xì)胞株等混合液,植入到受體裸大鼠背部皮下。對(duì)照組(n=20)供體制作方法同實(shí)驗(yàn)組,灌入等滲的生理鹽水,植入到受體裸大鼠背部皮下。于移植后30天獲取移植氣管標(biāo)本,制作病理切片及免疫組化。結(jié)果30天后獲取的實(shí)驗(yàn)組移植氣管標(biāo)本病理切片HE染色見(jiàn)氣管上皮細(xì)胞生長(zhǎng),免疫組織化學(xué)結(jié)果角蛋白抗體陽(yáng)性。而30天后獲取的對(duì)照組移植氣管標(biāo)本病理切片HE染色未見(jiàn)氣管上皮細(xì)胞生長(zhǎng),免疫組織化學(xué)結(jié)果角蛋白抗體陰性。結(jié)論同種異體大鼠氣管上皮細(xì)胞種植模型建立的成功,對(duì)肺移植或氣管移植術(shù)后閉塞性細(xì)支氣管炎的臨床研究和治療具有重要意義。
[Abstract]:Objective to establish a model of airway epithelium regeneration through allogeneic trachea transplantation in rats. Methods the trachea of Wistar rats was cold digested by trypsin and used as donor. A mixture of differentiated and mature tracheal epithelial cells was injected into the dorsal subcutaneous of the recipient nude rats. The donor of the control group was made in the same way as the experimental group, and the isosmotic saline was injected into the donor. The transplanted trachea specimens were obtained 30 days after transplantation, and pathological sections and immunohistochemistry were made. Results HE staining showed the growth of tracheal epithelial cells in the experimental group after 30 days. Immunohistochemical results showed that keratin antibody was positive, but 30 days later, there was no growth of tracheal epithelial cells in the pathological sections of trachea grafts in the control group. The results of immunohistochemistry showed that keratin antibody was negative. Conclusion the successful establishment of rat tracheal epithelial cell implantation model is of great significance for the clinical study and treatment of bronchiolitis obliterans after lung transplantation or trachea transplantation.
【學(xué)位授予單位】：蘭州大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2009
【分類號(hào)】：R653;R-332

【參考文獻(xiàn)】

相關(guān)期刊論文 前1條

1 李鑒,張德超,汪良駿,張大為,張汝剛;胸腺癌患者臨床病理特點(diǎn)和預(yù)后[J];中華腫瘤雜志;2004年04期

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本文編號(hào)：1649968