本文關鍵詞： 促紅細胞生成素 人骨髓間充質干細胞 增殖 細胞周期　出處：《蘭州大學》2008年碩士論文　論文類型：學位論文

【摘要】： 目的探討不同劑量的促紅細胞生成素(erythropoietin,EPO)對人骨髓間充質干細胞(mesenchymal stem cells,MSCs)增殖和細胞周期的影響。 方法采用Percoll淋巴細胞分離液,密度梯度離心法分離骨髓間充質干細胞;無血清條件下,以不同劑量0.25、0.50、1.00、2.00、4.00、8.00U/mL的促紅細胞生成素與骨髓間充質干細胞共培養(yǎng)為實驗組,不加促紅細胞生成素的骨髓間充質干細胞為對照組;24h、48h、72h后,MTT法檢測骨髓間充質干細胞的增殖情況;以10U/mL的促紅細胞生成素與骨髓問充質干細胞共培養(yǎng)72h后,流式細胞儀檢測MSCs的細胞周期。 結果本實驗骨髓間充質干細胞與促紅細胞生成素共培養(yǎng)后,骨髓間充質干細胞的增殖指數(shù)明顯增加,促紅細胞生成素呈時間、劑量依賴性促進骨髓間充質干細胞增殖,以4U/mL促紅細胞生成素促骨髓間充質干細胞增殖作用最明顯;與對照組比較,促紅細胞生成素干預組G_0/G_1期比例降低,S期和G_2/M期細胞比例增加,兩組差異顯著(P＜0.05)。 結論在促紅細胞生成素培養(yǎng)條件下可以促進更多的骨髓間充質干細胞進入DNA合成期,從而有更多的細胞分裂,產生大量的增殖細胞,并且這種影響呈時間、劑量依賴性。
[Abstract]:Objective to investigate the effects of different doses of erythropoietin (EPO) on the proliferation and cell cycle of mesenchymal stem cells (MSCs) in human bone marrow mesenchymal stem cells (MSCs). Methods Bone marrow mesenchymal stem cells (BMSCs) were isolated by Percoll lymphocyte isolation and density gradient centrifugation, and co-cultured with erythropoietin (EPO) and bone marrow mesenchymal stem cells (BMSCs) in different doses of 0.250.50U / mL and 4.00U / mL without serum. The proliferation of bone marrow mesenchymal stem cells (BMSCs) without erythropoietin was detected by MTT assay after 24 h, 48 h and 72 h, and then cultured with 10 U / mL Erythropoietin for 72 h. The cell cycle of MSCs was detected by flow cytometry. Results after co-culture of bone marrow mesenchymal stem cells with erythropoietin, the proliferation index of bone marrow mesenchymal stem cells increased significantly, and erythropoietin promoted the proliferation of bone marrow mesenchymal stem cells in a dose-dependent manner. The proliferation of bone marrow mesenchymal stem cells (BMSCs) stimulated by 4U / mL erythropoietin was the most obvious. Compared with the control group, the proportion of G0 / G1 phase of erythropoietin intervention group decreased the proportion of S phase and G2 / M phase, the difference between the two groups was significant (P < 0.05). Conclusion under the condition of erythropoietin culture, more bone marrow mesenchymal stem cells can enter DNA synthesis stage, thus more cells divide, and a large number of proliferative cells are produced, and this effect is time and dose dependent.
【學位授予單位】：蘭州大學
【學位級別】：碩士
【學位授予年份】：2008
【分類號】：R329

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本文編號：1535493