本文關鍵詞： 下丘腦室旁核 壓力感受性反射 甘氨酸 士的寧 腦部微量透析法　出處：《延邊大學》2009年碩士論文　論文類型：學位論文

【摘要】： 下丘腦室旁核(hypothalamic paraventricular nucleus,PVN)是內分泌系統(tǒng)和自主神經(jīng)系統(tǒng)的整合中樞,也是調節(jié)機體內環(huán)境穩(wěn)態(tài)和心血管活動的重要結構之一。據(jù)報道,PVN在正�；驊顟B(tài)下有著加強心血管活動的作用。但是,這些研究多數(shù)是直接或間接刺激PVN后觀察了心血管活動的變化,沒有對參與心血管活動調節(jié)的PVN內的神經(jīng)遞質做進一步的研究,尤其對PVN參與正常壓力感受性反射調節(jié)的神經(jīng)化學機制報道稀少。甘氨酸是中樞神經(jīng)系統(tǒng)中分布較廣泛的氨基酸類神經(jīng)遞質,研究表明包括PVN在內的下丘腦有甘氨酸能神經(jīng)元的分布,但甘氨酸在這些部位的作用及其機制尚不清楚。為了闡明PVN參與正常壓力感受性反射調節(jié)的部分神經(jīng)化學機制,也為了明確PVN內的甘氨酸在此反射調節(jié)中的作用,本實驗利用清醒大鼠,在靜脈注射苯腎上腺素誘發(fā)壓力感受性反射的同時利用腦部微量透析法和高效液相色譜分析法,觀察PVN區(qū)細胞外液中的甘氨酸(Gly)含量的變化。其次,通過PVN區(qū)灌流甘氨酸受體阻斷劑士的寧(strychnine)和激動劑甘氨酸,進一步探討了PVN區(qū)甘氨酸參與壓力感受性反射中樞調節(jié)的具體機制。 實驗結果如下: 1.靜脈注射苯腎上腺素誘發(fā)壓力感受性反射,血壓由正常的(99.5±14.2)mmHg上升到(149.8±19.5)mmHg(P＜0.01),心率反射性地從(400.8±33.1)次/分下降到(273.4±40.8)次/分(P＜0.01);PVN區(qū)細胞外液中甘氨酸水平由注射前的100%升高到162.89%±27.93%(P＜0.05)。 2.向PVN區(qū)灌流甘氨酸受體阻斷劑士的寧(100μmol/L)20分鐘,同時靜脈給予與對照組相同劑量的苯腎上腺素誘發(fā)壓力感受性反射。和對照組相比,阻斷劑使用組,血壓升高的幅度明顯升高(P＜0.001),而心率下降的幅度卻明顯降低(P＜0.001),導致壓力感受性反射的敏感性(ΔHR/ΔMAP)明顯減少(P＜0.001)。 3.向PVN區(qū)灌流甘氨酸(1mmol/L)20分鐘,同時靜脈給予與對照組相同劑量的苯腎上腺素誘發(fā)壓力感受性反射。和對照組相比,甘氨酸使用組,血壓升高的幅度升高不明顯(P＞0.05),而心率下降的幅度卻明顯升高(P＜0.001),導致壓力感受性反射的敏感性(ΔHR/ΔMAP)明顯增加(P＜0.001)。 結論: PVN內的甘氨酸通過士的寧敏感的甘氨酸受體對壓力感受性反射活動起易化作用。
[Abstract]:Hypothalamic paraventricular nucleus (PVN) is the integration center of endocrine system and autonomic nervous system. PVN is also one of the important structures that regulate homeostasis and cardiovascular activity in the body. PVN is reported to enhance cardiovascular activity in normal or stressed state. Most of these studies observed changes in cardiovascular activity after direct or indirect stimulation of PVN, with no further study of neurotransmitters in PVN involved in cardiovascular regulation. In particular, there are few reports on the neurochemical mechanism of PVN involved in normal baroreflex regulation. Glycine is a widely distributed amino acid neurotransmitter in the central nervous system. Studies have shown that the hypothalamus, including PVN, has the distribution of glycinergic neurons, but the role of glycine in these sites and its mechanism are not clear. In order to elucidate some neurochemical mechanisms of PVN involved in the regulation of normal baroreflex. In order to elucidate the role of glycine in the regulation of reflex in PVN, the baroreflex was induced by intravenous injection of phenylephrine in awake rats, and microdialysis and high performance liquid chromatography were used in this study. The changes of glycine glycine in extracellular fluid of PVN region were observed. Secondly, the glycine receptor blocker strychnine (strychnine) and the agonist glycine were perfused through the PVN region. Furthermore, the mechanism of glycine in PVN region involved in baroreflex regulation was discussed. The results are as follows:. 1. The blood pressure increased from 99.5 鹵14.2mm Hg to 149.8 鹵19.5mm Hg (P < 0.01), and the heart rate reflex decreased from 400.8 鹵33.1 times / min to 273.4 鹵40.8 times / min (P < 0.01). The level of glycine in extracellular fluid increased from 100% before injection to 162.89% 鹵27.931g / min (P < 0.05). 2. The glycine receptor blocker, strychnine, was perfused into the PVN area for 100 渭 mol/L)20 minutes, and the baroreflex was induced by the same dose of phenylephrine as that in the control group. The amplitude of blood pressure rise was significantly increased (P < 0.001), while the decrease of heart rate was significantly decreased (P < 0.001), which resulted in a marked decrease in the sensitivity of baroreflex (螖 HR- / 螖 MAPP) (P < 0.001). 3. One mmol / L of glycine was perfused into the PVN area for 20 minutes, and the same dose of phenylephrine was given to induce baroreflex in the control group for 20 minutes. Compared with the control group, the glycine group was given the same dose of phenylephrine to induce baroreflex. The amplitude of elevation of blood pressure was not significant (P > 0.05), but the decrease of heart rate was significantly increased (P < 0.001), which resulted in a marked increase in the sensitivity of baroreflex (螖 HR- / 螖 MAPP) (P < 0.001). Conclusion:. Glycine in PVN facilitates baroreflex activity through strychnine-sensitive glycine receptors.
【學位授予單位】：延邊大學
【學位級別】：碩士
【學位授予年份】：2009
【分類號】：R33

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