本文關(guān)鍵詞： 胰島素 絞股藍(lán)愈傷組織細(xì)胞 糖尿病 普洱茶 脂質(zhì)體疫苗　出處：《吉林大學(xué)》2010年博士論文　論文類(lèi)型：學(xué)位論文

【摘要】： 新型降糖活性物質(zhì)的研究: 治療糖尿病包括以下兩種方式:1,使用胰島素;2,使用其他降糖藥物。對(duì)應(yīng)這兩種方式,本文構(gòu)建和篩選了兩種降糖活性物質(zhì),并分別對(duì)這兩種物質(zhì)的降糖效果進(jìn)行了研究。對(duì)于CTB-人胰島素融合蛋白,構(gòu)建并篩選到含CTB-BA的陽(yáng)性細(xì)胞并采用了Balb/c小鼠動(dòng)物試驗(yàn)檢測(cè)其降血糖活性,表明陽(yáng)性細(xì)胞具有較強(qiáng)的降血糖活性。對(duì)于普洱茶,首先對(duì)各種茶在體外對(duì)PTP-1B的抑制作用進(jìn)行了考察,并得到對(duì)PTP-1B抑制作用最強(qiáng)的普洱茶。對(duì)普洱茶在db/db小鼠上的降糖效果進(jìn)行研究。普洱茶水飲用組表現(xiàn)出很高的降血糖活性,而采用灌胃給藥組亦表現(xiàn)出一定的降糖活性。對(duì)普洱茶的減肥作用進(jìn)行研究,飲用普洱茶具有減肥作用。對(duì)普洱茶對(duì)糖尿病患者和健康受試者的降血糖作用進(jìn)行了研究,發(fā)現(xiàn)對(duì)于糖尿病受試者組,普洱茶顯著地降低了他們的空腹血糖、餐后2h血糖和HbA1c(%),健康受試者組則沒(méi)有明顯的變化。對(duì)試驗(yàn)前后血Ca~(2+),K~+濃度進(jìn)行檢測(cè),實(shí)驗(yàn)前后血Ca~(2+),K~+的濃度均在正常范圍內(nèi),證明喝茶不會(huì)造成Ca~(2+),K~+流失。新型脂質(zhì)體疫苗的研究: 以脂質(zhì)體作為流感裂解疫苗佐劑,采用凍干重建法,制備了流感脂質(zhì)體疫苗。實(shí)驗(yàn)表明,相同劑量下,脂質(zhì)體流感疫苗組與相應(yīng)非脂質(zhì)體流感疫苗組相比,可激發(fā)2-4倍的抗體,抗體高峰持續(xù)時(shí)間更長(zhǎng),在脾細(xì)胞增值比率,CD4+水平及CD4+/CD8+比例,以及細(xì)胞因子(IL-4、IFNγ)水平上均有顯著的增強(qiáng)作用。脂質(zhì)體流感疫苗不但能夠刺激機(jī)體產(chǎn)生較無(wú)佐劑疫苗更高效持久的體液免疫,而且能夠激發(fā)機(jī)體產(chǎn)生細(xì)胞免疫。
[Abstract]:Studies on novel hypoglycemic active substances:. There are two ways to treat diabetes: 1: 1, insulin 2, and other hypoglycemic drugs. For these two methods, we constructed and screened two hypoglycemic active substances. For CTB- human insulin fusion protein, positive cells containing CTB-BA were constructed and screened, and the hypoglycemic activity of CTB- human insulin fusion protein was detected by Balb/c mice test. The results showed that the positive cells had strong hypoglycemic activity. For Pu'er tea, the inhibitory effects of various kinds of tea on PTP-1B in vitro were investigated. Pu'er tea, which has the strongest inhibitory effect on PTP-1B, was obtained. The hypoglycemic effect of Pu'er tea on db/db mice was studied. The drinking group of Pu'er tea water showed high hypoglycemic activity. The effect of drinking Pu'er tea on reducing blood sugar was studied, and the hypoglycemic effect of Pu'er tea on diabetic patients and healthy subjects was studied. It was found that in diabetic subjects, Pu'er tea significantly decreased their fasting blood glucose, 2 h postprandial blood glucose and HbA1cU, but not in healthy subjects. Before and after the experiment, the concentration of blood Ca~(2 was in the normal range, which proved that drinking tea would not cause the loss of Ca~(2. Study on novel Liposome Vaccine:. Using liposome as adjuvant of influenza lytic vaccine, the influenza liposome vaccine was prepared by freeze-dried reconstruction method. The experiment showed that the liposome influenza vaccine group was compared with the corresponding non-liposome influenza vaccine group at the same dosage. The antibody can be stimulated 2-4 times, the antibody peak duration is longer, in splenocyte proliferation ratio, CD4 level and CD4 / CD8 ratio, The liposome influenza vaccine can not only stimulate the body to produce more efficient and lasting humoral immunity than the adjuvant vaccine, but also stimulate the body to produce cellular immunity.
【學(xué)位授予單位】：吉林大學(xué)
【學(xué)位級(jí)別】：博士
【學(xué)位授予年份】：2010
【分類(lèi)號(hào)】：R392.1

【引證文獻(xiàn)】

相關(guān)期刊論文 前1條

1 趙新軍;蔣中英;;原子力顯微鏡研究糖對(duì)脂質(zhì)體的穩(wěn)定作用[J];伊犁師范學(xué)院學(xué)報(bào)(自然科學(xué)版);2013年01期

相關(guān)碩士學(xué)位論文 前1條

1 劉洪卿;納米乳與普洱茶提取物作為H3N2流感病毒滅活疫苗佐劑的免疫效果及安全性的初步研究[D];北京協(xié)和醫(yī)學(xué)院;2013年

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本文編號(hào)：1495055