本文關(guān)鍵詞： 胰島素抵抗 低鈉鹽 胰島素信號(hào)通路 表達(dá)譜芯片 局部網(wǎng)絡(luò)　出處：《天津科技大學(xué)》2008年碩士論文　論文類(lèi)型：學(xué)位論文

【摘要】： 隨著人類(lèi)基因組測(cè)序工作的完成,基因與飲食的關(guān)系已經(jīng)成為營(yíng)養(yǎng)學(xué)研究的新熱點(diǎn)。在流行病學(xué)研究中,發(fā)現(xiàn)高血壓與肥胖均屬于多基因疾病,且都存在胰島素抵抗,即胰島素信號(hào)通路傳導(dǎo)異常。限鹽作為有效的非藥物手段,在控制血壓、肥胖及緩減胰島素抵抗方面有著積極作用�；谝陨系难芯�,本論文應(yīng)用基因芯片技術(shù),從分子水平,揭示低鈉鹽飲食對(duì)胰島素信號(hào)通路相關(guān)基因表達(dá)情況的影響,并建立基因間相互關(guān)聯(lián)的局部網(wǎng)絡(luò)。 論文中所用的低鈉鹽由川崎食品有限公司提供,其N(xiāo)aCl含量為50%左右。在監(jiān)督下,用這種鹽代替普通食鹽,針對(duì)正常人群、高血壓人群、以及高血壓并發(fā)肥胖人群,共9人,進(jìn)行12周的實(shí)驗(yàn)。采集受試者食用前后血樣,進(jìn)行芯片實(shí)驗(yàn)。 本論文通過(guò)生物信息統(tǒng)計(jì),確定血液中表達(dá)的胰島素信號(hào)通路相關(guān)基因共114個(gè)。針對(duì)高血壓人群與正常人群,應(yīng)用博奧生物有限公司的22K人類(lèi)全基因組芯片。主要的變化基因Akt2、Akt3、GSK3、GCK、HK2、以及SOS參與了相關(guān)通路：PI-3K/Akt Signaling Pathway, Focal Adhesion, T Cell Receptor Signaling Pathway, JAK-STAT Signaling Pathway。針對(duì)高血壓并發(fā)肥胖患者人群,應(yīng)用康成生物工程公司針對(duì)性更強(qiáng)的芯片,僅對(duì)114個(gè)胰島素信號(hào)通路相關(guān)基因進(jìn)行檢測(cè)。由表達(dá)明顯變化的基因建立基因局部網(wǎng)絡(luò),主要涉及到的通路有：PI-3K/Akt Signaling Pathway, Focal Adhesion,B Cell Reception Signaling Pathway, long-term depression。通過(guò)對(duì)這些基因局域網(wǎng)絡(luò)的分析,發(fā)現(xiàn)這些基因表達(dá)的變化對(duì)胰島素信號(hào)相關(guān)通路的傳導(dǎo)有一定改善作用,調(diào)控細(xì)胞的活性,改善葡萄糖的攝取和運(yùn)輸,在一定程度上能緩減胰島素抵抗。希望本論文能為限鹽研究提供新的思路和線(xiàn)索。
[Abstract]:With the completion of human genome sequencing, the relationship between gene and diet has become a new focus in nutrition research. In epidemiological studies, hypertension and obesity are found to be polygenic diseases. And there is insulin resistance, that is, abnormal insulin signaling pathway. Salt limitation, as an effective non-drug means, plays an active role in controlling blood pressure, obesity and reducing insulin resistance. Based on the above research. In this paper, the effects of low-sodium diet on the expression of genes related to insulin signaling pathway were revealed at the molecular level by using gene chip technology, and a local network of interrelationship between genes was established. The low sodium salt used in this paper is provided by Kawasaki Food Co., Ltd., its NaCl content is about 50%. Under supervision, this salt is used to replace the common salt. And 9 subjects with hypertension and obesity were tested for 12 weeks. Blood samples before and after eating were collected and microarray experiments were carried out. In this paper, 114 genes related to insulin signaling pathway were identified by bioinformatics. Using the 22K human genome microarray of Boo Biological Co., Ltd., the main mutated gene Akt2, Akt3, GSK3, GSK3, GCK3, HK2. And SOS was involved in the related pathways:: PI-3K / Akt Signaling Pathway, Focal Adhesion. T Cell Receptor Signaling Pathway. JAK-STAT Signaling Pathway. for people with high blood pressure and obesity, use a more targeted chip from Kangcheng Bioengineering. Only 114 genes associated with insulin signaling pathway were detected. The main pathways involved are: PI-3K / Akt Signaling Pathway, Focal Adhesion. B Cell Reception Signaling Pathway. Long-term depression.According to the analysis of the local network of these genes, it is found that the changes in the expression of these genes have a certain effect on the transduction of insulin signal-related pathways. Regulation of cell activity and improvement of glucose uptake and transport can reduce insulin resistance to a certain extent. It is hoped that this paper can provide new ideas and clues for salt limitation research.
【學(xué)位授予單位】：天津科技大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2008
【分類(lèi)號(hào)】：R341

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