本文關(guān)鍵詞： ADAMTS-1 排卵 小鼠　出處：《華中科技大學(xué)》2008年碩士論文　論文類型：學(xué)位論文

【摘要】： 目的:探討促性腺激素預(yù)處理的小鼠排卵過程中ADAMTS-1在卵巢中的表達(dá)與排卵的關(guān)系。 方法:將40只22-24天大小的C57/BL6雌性小鼠隨機(jī)分成10組,分別為孕馬血清促性腺激素(PMSG)組(D1組)、注射hCG后2h、4h、8h、12h、16h、24h、48h、72h(D2組-D9組)及對(duì)照組生理鹽水組(D0組),每組4只小鼠。采用原位雜交進(jìn)行組織學(xué)定位,逆轉(zhuǎn)錄聚合酶鏈反應(yīng)(RT-PCR)進(jìn)行半定量,在mRNA水平上檢測(cè)排卵過程中ADAMTS-1在小鼠卵巢中的動(dòng)態(tài)表達(dá)。 結(jié)果: ADAMTS-1主要表達(dá)于卵巢表面大卵泡的顆粒細(xì)胞,而在卵巢中央的小卵泡和閉鎖卵泡沒有表達(dá)。對(duì)照組(D0組)、PMSG組(D1組)、注射hCG后48 h和72 h(D8組、D9組)均無目的基因ADAMTS-1的表達(dá),注射hCG后2h、4h、8h、12h、16h、24h(D2組-D7組)均有目的基因ADAMTS-1的表達(dá),且從注射hCG后2 h至12 h卵巢ADAMTS-1mRNA的表達(dá)逐漸增強(qiáng),注射hCG后12 h(D5組)達(dá)到高峰,其ADAMTS-1mRNA表達(dá)的相對(duì)值為(1.357±0.328),與各組比較均有極顯著差異( P 0.05),此后表達(dá)逐漸下降,至注射hCG后48 h(D8組)無表達(dá),但D2、D3、D4、D6、D7組之間無顯著性差異( P 0.05)。 結(jié)論: ADAMTS-1在注射hCG后12h達(dá)到高峰,此時(shí)正是促性腺激素預(yù)處理的小鼠即將排卵的時(shí)間。ADAMTS-1可能是排卵過程中重要的信號(hào)傳導(dǎo)分子。
[Abstract]:Aim: to investigate the relationship between ADAMTS-1 expression and ovulation during ovulation of mice pretreated with gonadotropin. Methods: forty C57 / BL6 female mice aged 22 to 24 days were randomly divided into 10 groups: group D 1 (gestational horse serum gonadotropin) group and group D 1, 2 h after injection of hCG. Group D _ (9) and control group (n = 4) and normal saline group (n = 4). Histological localization was performed by in situ hybridization. Reverse transcriptase polymerase chain reaction (RT-PCR) was used to detect the dynamic expression of ADAMTS-1 in mouse ovary at mRNA level. Results: ADAMTS-1 was mainly expressed in granulosa cells of large follicles on the surface of ovary, but not in small follicles and atretic follicles in central ovary. In PMSG group, there was no expression of ADAMTS-1 at 48 h and 72 h after hCG injection, and the expression of ADAMTS-1 was found at 4 h after hCG injection. The expression of target gene ADAMTS-1 was observed in D _ 2 group (n = 12) and D _ 2 group (n = 24). The expression of ovarian ADAMTS-1mRNA increased gradually from 2 h to 12 h after hCG injection, and reached the peak in group D 5 12 h after hCG injection. The relative value of ADAMTS-1mRNA expression was 1.357 鹵0.328, which was significantly different from that of each group (P 0.05), and then decreased gradually. There was no expression of D8 at 48 h after hCG injection, but there was no significant difference between D2D3, D4, D6 and D7 groups (P 0.05). Conclusion: ADAMTS-1 reached its peak at 12 hours after hCG injection. This is the time of ovulation in gonadotropin pretreated mice. ADAMTS-1 may be an important signal transduction molecule during ovulation.
【學(xué)位授予單位】：華中科技大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2008
【分類號(hào)】：R341

【引證文獻(xiàn)】

相關(guān)碩士學(xué)位論文 前1條

1 烏吉斯古楞;蒙古羊多羔性狀候選基因ADAMTS1的遺傳效應(yīng)分析[D];內(nèi)蒙古農(nóng)業(yè)大學(xué);2013年

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本文編號(hào)：1453817