本文關(guān)鍵詞： 感染 腸功能紊亂 動物模型 內(nèi)臟敏感性 小腸通透性 結(jié)腸通透性　出處：《安徽醫(yī)科大學》2009年碩士論文　論文類型：學位論文

【摘要】： 背景和目的:腸易激綜合征是最常見的功能性胃腸病,其病因和發(fā)病機制至今仍不明確。目前對該病的研究仍缺乏一個穩(wěn)定易行,可靠可行的動物模型。此項研究的目的就是:建立福氏志賀菌感染后腸功能紊亂大鼠模型,在此基礎(chǔ)上探討腸功能紊亂大鼠腸道通透性的變化以及腸黏膜IL-1β的表達,為感染后腸易激綜合征的病因和發(fā)病機制提供依據(jù)。方法:將40只雄性Wistar大鼠隨機分為實驗組和對照組,實驗組用5*109cfu\ml 4型福氏志賀菌2ml灌胃,對照組大鼠予2ml生理鹽水灌胃。于灌胃后每日觀察大鼠大便的性狀,并于第1天、第3天、第14天、第21天行大便培養(yǎng),在感染后第21天、42天和63天給大鼠行直結(jié)腸球囊擴張觀察其腹部回撤反射中引起大鼠腹部抬離桌面和弓背的最小容量閾值來反映感染后大鼠的內(nèi)臟敏感性。感染后第68天用乳果糖、甘露醇和三氯蔗糖配置的溶液給大鼠灌胃,用氣相色譜法檢測大鼠灌過糖溶液后5小時尿中乳果糖和甘露醇的排泄率比值(L/M)及第6小時至24小時尿中三氯蔗糖的排泄量來分別反應小腸道通透性和結(jié)腸通透性的變化。上述實驗全部結(jié)束后將所有大鼠處死,取末端回腸及近端結(jié)腸行組織病理學檢查觀察炎癥情況并采用免疫組化S-P法檢查腸黏膜IL-1β的表達。結(jié)果:(1)用4型福氏志賀菌感染后第1~2天大鼠出現(xiàn)稀便和粘液便,7~10天就基本恢復正常,在感染后第21天行內(nèi)臟敏感性后大鼠再次出現(xiàn)大便變稀、不成形帶粘液,有18只大鼠反復出現(xiàn)大便性狀改變直到實驗結(jié)束。對照組大鼠在整個實驗期間大便一直正常。感染組大鼠在感染后第1天及第3天大便培養(yǎng)出致病菌,在第14天及第21天大便培養(yǎng)均為陰性。在感染后第21天、42天、63天測得引起大鼠腹部抬離桌面和弓背的最小容量閾值較對照組均明顯減低(P0.05)。組織病理學檢查未見有明顯的中性粒細胞浸潤及間質(zhì)水腫。(2)采用氣相色譜法測得感染后大鼠5小時尿中L/M值和第6小時至24小時尿中的三氯蔗糖的排泄量較對照組均明顯增加(P0.05)。(3)感染后大鼠末端回腸及近端結(jié)腸黏膜IL-1β的表達較對照組均明顯增加(P0.05)。結(jié)論:(1)福氏志賀菌感染后大鼠腸功能紊亂模型建立成功。(2)腸功能紊亂大鼠小腸通透性及結(jié)腸通透性明顯增加,腸黏膜IL-1β表達增加,說明感染后腸功能紊亂大鼠存在腸黏膜屏障的破壞和腸黏膜的低度炎癥。
[Abstract]:Background and objective: irritable bowel syndrome is the most common functional gastrointestinal disease, its etiology and pathogenesis are still unclear. A reliable and feasible animal model. The aim of this study was to establish a rat model of intestinal dysfunction after Shigella flexneri infection. On this basis, the changes of intestinal permeability and the expression of IL-1 尾 in intestinal mucosa in rats with intestinal dysfunction were studied. Methods: 40 male Wistar rats were randomly divided into experimental group and control group. The rats in the experimental group were given 2 ml of Shigella flexneri type 4 and the rats in the control group were given 2ml normal saline. The feces were observed daily and on the first day after the rats were fed with 5 pieces of 109cfu\ ml 4 Shigella flexneri. Feces were cultured on day 3, day 14 and day 21 after infection. 42 and 63 days of balloon dilatation of straight colon were performed in rats to observe the minimum volume threshold of abdominal withdrawal reflex to reflect the visceral sensitivity of infected rats. 68 days after infection, the minimum volume threshold of abdominal lift away from the tabletop and back of the bowels of rats was observed. With fructose. Rats were fed with mannitol and sucrose trichloride solution. The excretion rate ratio of lactofructose to mannitol in urine of rats was determined by gas chromatography after 5 hours of glucose solution administration. The excretion of sucrose in urine from 6 hours to 24 hours was used to reflect the changes of intestinal permeability and colon permeability respectively. All the rats were killed after all the above experiments were completed. The distal ileum and proximal colon were taken for histopathological examination to observe inflammation and immunohistochemical S-P method was used to detect the expression of IL-1 尾 in intestinal mucosa. The rats infected with Shigella flexneri type 4 showed dilute stool and mucus stool on the 1st day after infection. 10 days after infection, the rats returned to normal basically. After 21 days of infection with visceral sensitivity, the rats again appeared defecation thinning and anamorphic mucus. There were 18 rats repeatedly appeared the change of defecation until the end of the experiment. The control group rats had normal defecation during the whole experiment. The infected rats in the first and third day after infection feces culture pathogenic bacteria. Stool culture was negative on the 14th and 21st day and 42 days after infection. At 63 days, the minimum volume threshold of abdominal lift away from the table and back of the rat was significantly lower than that of the control group (P 0.05). No obvious neutrophil infiltration and interstitial edema were found in histopathological examination. 2) the values of L / M in 5 hours urine and the excretion of sucralose in urine from 6 hours to 24 hours after infection were significantly increased by gas chromatography compared with the control group. The expression of IL-1 尾 in the distal ileum and proximal colon of the infected rats was significantly higher than that in the control group (P 0.05). Conclusion the intestinal dysfunction model of rats infected with Shigella flexneri was successfully established. (. 2) intestinal permeability and colon permeability were significantly increased in rats with intestinal dysfunction. The increased expression of IL-1 尾 in intestinal mucosa showed that the intestinal mucosal barrier was destroyed and the intestinal mucosal inflammation occurred in the rats with intestinal dysfunction after infection.
【學位授予單位】：安徽醫(yī)科大學
【學位級別】：碩士
【學位授予年份】：2009
【分類號】：R574;R-332

【參考文獻】

相關(guān)期刊論文 前5條

1 郭敏;李延青;于秀娟;孔慶蓮;郭玉婷;左秀麗;曾娟;;大鼠急性腸道感染后腸功能紊亂動物模型的建立[J];山東大學學報(醫(yī)學版);2006年06期

2 王利華,方秀才,潘國宗,溫淑豪,楊曉鷗;腸道感染與應激對實驗兔腸運動功能的影響[J];胃腸病學和肝病學雜志;2004年04期

3 潘國宗,魯素彩,柯美云,韓少梅,郭慧平,方秀才;北京地區(qū)腸易激綜合征的流行病學研究:一個整群、分層、隨機的調(diào)查[J];中華流行病學雜志;2000年01期

4 尉秀清,陳e，

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