本文關(guān)鍵詞：肺炎克雷伯桿菌噬菌體的分離鑒定和生物學特性的研究　出處：《吉林大學》2009年碩士論文　論文類型：學位論文

  更多相關(guān)文章： 肺炎克雷伯桿菌 噬菌體 最佳感染復(fù)數(shù) 生長曲線

【摘要】： 抗生素一直被認為是治療細菌感染性疾病最有力的武器,但隨著致病菌對大多數(shù)抗生物產(chǎn)生抗性的出現(xiàn),細菌的耐藥性已成為現(xiàn)代醫(yī)學中的一個非常嚴峻的問題。人們擔憂人類正重新進入“前抗生素”時代。噬菌體作為一種新興的治療方法,已經(jīng)越來越受到人們的關(guān)注。噬菌體對細菌具有高度專一性,當噬菌體侵染細菌時,可在細菌中繁殖并殺死細菌,而對動植物沒有毒性。 本研究以肺炎克雷伯桿菌噬菌體為主要內(nèi)容,從居民區(qū)污水中分離出二株肺炎克雷伯桿菌噬菌體,分別命名為K1BP108和K1BP109。同時對其生物學性狀進行了初步的研究。結(jié)果表明,利用噬斑法能獲得比較純的噬菌體,電鏡照片顯示為立體對稱型,未見明顯的尾部;所分離到的肺炎克雷伯桿菌噬菌體的基因組為雙鏈DNA,大小約25kb左右;在pH6.5~10的范圍內(nèi)噬菌體保持較好的裂解活性;在溫度60℃條件下,噬菌體K1BP108穩(wěn)定,生長曲線顯示噬菌體感染宿主菌的潛伏時間為31分鐘,爆發(fā)時間為47分鐘;在紫外線照射下18分鐘噬菌體全部被滅活。 肺炎克雷伯桿菌是革蘭氏陰性菌,是醫(yī)院內(nèi)感染重要的病原菌之一,其耐藥性也呈逐年增加趨勢,開發(fā)和研究針對肺炎克雷伯桿菌的噬菌體制劑,無疑是拓展抵御細菌感染的新領(lǐng)域。本研究探討了肺炎克雷伯桿菌噬菌體的生物學特性,為進一步研制和開發(fā)針對肺炎克雷伯桿菌感染,特別是針對耐藥和多重耐藥菌株的噬菌體打下基礎(chǔ)。
[Abstract]:Antibiotic treatment of bacterial infections has been considered to be the most powerful weapon of disease, but with the most anti biological resistance emergence of pathogenic bacteria, bacterial resistance has become a serious problem in modern medicine. People worry about "era anti humanity is re entering the" students. As a new phage the treatment method has attracted more and more attention. The phage has a high degree of specificity of bacteria, when phage infection bacteria, in bacteria breeding and kill bacteria, and no toxicity to plants and animals.
In this study, Klebsiella pneumoniae phage as the main content, two strains of Klebsiella pneumoniae phages from residential areas in the sewage, which were named as K1BP108 and K1BP109. also conducted a preliminary study on its biological characters. The results showed that by plaque assay can obtain relatively pure phage as electron micrograph the three-dimensional symmetrical type, there is no obvious tail; isolated Klebsiella pneumoniae phage genome into double stranded DNA, the size is about 25KB; in the range of pH6.5~10 to maintain good phage lytic activity; at a temperature of 60 DEG C, phage K1BP108 stable, growth curve showed that the latent time of host bacteria phage infection for 31 minutes, the onset time is 47 minutes; 18 minutes under UV irradiation were all phage inactivated.
Klebsiella pneumoniae is a gram negative bacterium, is one of the important pathogens of nosocomial infection and its drug resistance has increased year by year, the research and development of the phage preparations of Klebsiella pneumoniae, is expanding new areas against bacterial infection. This study explores the biological characteristics of Klebsiella pneumoniae phage, for further research and development for Klebsiella pneumoniae infection, especially for drug resistance and multiple drug resistant strains of phage to lay the foundation.

【學位授予單位】：吉林大學
【學位級別】：碩士
【學位授予年份】：2009
【分類號】：R516;R378

【引證文獻】

相關(guān)碩士學位論文 前1條

1 劉斌;廈門港、波羅的海與東太平洋TCBS類群細菌多樣性及相關(guān)噬菌體的生物學特性研究[D];國家海洋局第三海洋研究所;2010年

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本文編號：1433866