本文關(guān)鍵詞：電刺激大鼠島葉對心率變異性的影響　出處：《中國醫(yī)科大學(xué)》2008年碩士論文　論文類型：學(xué)位論文

  更多相關(guān)文章： 心率變異性 島葉 電刺激 預(yù)刺激

【摘要】： 前言 在竇性心律的一定時(shí)間內(nèi),這種逐次心動(dòng)周期之間的時(shí)間變異數(shù)即為心率變異性(heart rate variability,HRV)。產(chǎn)生心率波動(dòng)的因素非常復(fù)雜,包括腦的高級神經(jīng)活動(dòng)、中樞神經(jīng)系統(tǒng)的自發(fā)性節(jié)律活動(dòng)、呼吸活動(dòng)以及由壓力、化學(xué)感受器導(dǎo)入的心血管反射活動(dòng)等。臨床醫(yī)學(xué)還證明:急性心肌梗塞、心臟病患者的猝死、充血性心衰、高血壓、冠心病等心血管疾病,甚至糖尿病患者的HRV都較正常人有顯著的降低。由此可見,HRV的研究對心血管疾病的早期診斷、病中監(jiān)護(hù)及預(yù)后評估有重要意義。 島葉是控制交感神經(jīng)和副交感神經(jīng)介導(dǎo)的心血管調(diào)節(jié)最重要的皮層區(qū)域,島葉損傷會引起一系列心率變異性參數(shù)的變化,包括SDNN,RMSSD降低,LF/HF升高以及夜間平均心率明顯增加,血清去甲腎上腺素水平升高和QT間期延長以及復(fù)雜的心律失常,甚至猝死。這些現(xiàn)象均說明島葉損傷可以引起交感—迷走神經(jīng)平衡失調(diào),進(jìn)而出現(xiàn)病理性交感神經(jīng)激活。預(yù)適應(yīng)現(xiàn)象(Preconditioning,PC)是指機(jī)體或者組織預(yù)先受到輕微的刺激或者損傷后,對后期較強(qiáng)的刺激或損傷產(chǎn)生耐受的現(xiàn)象。預(yù)適應(yīng)原理可以使我們針對不同的應(yīng)激采用合適的措施來提高自身對應(yīng)激的適應(yīng)性,以防范應(yīng)激產(chǎn)生的傷害效應(yīng)。本實(shí)驗(yàn)通過觀察島葉接受電刺激及預(yù)刺激后大鼠HRV的改變及其頻譜成份的變化,來判斷電刺激及預(yù)刺激在大鼠HRV調(diào)節(jié)中的作用。 實(shí)驗(yàn)方法 健康雄性Wistar大鼠40只,體重250—300g,由中國醫(yī)科大學(xué)實(shí)驗(yàn)動(dòng)物中心提供。在實(shí)驗(yàn)前三天將大鼠置于一避音房間,給予12h:12h光照—黑暗周期(早8:00—20:00光照),保持室溫24±2℃,濕度(55±5)%。大鼠單籠飼養(yǎng),自由進(jìn)食、水,在實(shí)驗(yàn)中隨機(jī)分組。測量心電圖前三天讓大鼠在實(shí)驗(yàn)室適應(yīng)1—2小時(shí)。 隨機(jī)抽取10只大鼠,采用針形電極給予500μA電流20分鐘,作為500μA組。隨機(jī)抽取10只大鼠,采用針形電極給予1000μA電流20分鐘,作為1000μA組。隨機(jī)抽取10只大鼠,在給予1000μA電刺激前,給予50μA電流20分鐘,作為預(yù)刺激組。隨機(jī)抽取10只大鼠采用上述方法插入電極,但是不通電流,作為假手術(shù)對照組。然后分別測量各組ECG,用隨機(jī)附帶的分析軟件分析HRV,得到HRV各指標(biāo)的數(shù)據(jù)。數(shù)據(jù)用SPSS軟件處理。 實(shí)驗(yàn)結(jié)果 500μA組同假手術(shù)組相比較HRV時(shí)域指標(biāo)中SDNN(P＜0.05);RMSSD(P＞0.05)下降;頻域指標(biāo)中TP(P＜0.05)下降;而LF/HF升高(P＜0.01)。1000μA組同假手術(shù)組相比較HRV時(shí)域指標(biāo)中SDNN(P＜0.05),RMSSD(P＞0.05)下降;頻域指標(biāo)中TP(P＜0.05)下降;而LF/HF升高(P＜0.01)。預(yù)刺激組SDNN(P＜0.01),RMSSD(P＜0.05),TP(P＜0.05),LF/HF(P＞0.05)均升高,且LF/HF升高幅度不及1000μA刺激組,提示預(yù)刺激可以削弱電刺激島葉造成的交感神經(jīng)活性升高。 結(jié)論 1、電刺激島葉使大鼠HRV時(shí)域指標(biāo)中SDNN、RMSSD下降;頻域指標(biāo)TP下降,但是LF/HF升高,而且這種變化是可逆的。 2、電刺激島葉可通過作用于自主神經(jīng)系統(tǒng),增加交感神經(jīng)張力,降低迷走神經(jīng)張力,使交感和迷走神經(jīng)功能失調(diào)而使HRV降低。 3、預(yù)刺激可以減輕電刺激對HRV的影響,并且使HRV參數(shù)值較快恢復(fù)正常。
[Abstract]:Preface
In a certain period of time to sinus rhythm within the time period between successive cardiac number variation is the heart rate variability (heart rate, variability, HRV). The factors causing the fluctuation of heart rate is very complex, including senior neural activity of the brain, spontaneous rhythm of central nervous system activity, breathing activity and by pressure chemoreceptor the cardiovascular reflex activities. Clinical medicine has proved that acute myocardial infarction and sudden death in patients with heart disease, hypertension, congestive heart failure, coronary heart disease and other cardiovascular diseases, even in patients with diabetes compared with normal HRV were significantly reduced. Therefore, the early diagnosis of cardiovascular disease in HRV research, it is important to evaluate the monitoring and the prognosis of disease.
The insula is control of sympathetic and parasympathetic nerve mediated cardiovascular regulating cortical regions most important changes, the insular injury can cause a series of HRV parameters including SDNN, RMSSD decreased, LF/HF increased and nocturnal mean heart rate increased significantly, serum norepinephrine epinephrine levels and prolonged QT interval and complex arrhythmias, or sudden death. These phenomena explain that insular damage may be caused by sympathetic and vagal imbalance, and pathological sympathetic activation. Preconditioning (Preconditioning, PC) refers to the body or organization in advance by the slight stimulation or injury, produce the phenomenon of tolerance to strong stimulation or late injury. Principle of preconditioning can make us different stress to adopt appropriate measures to improve their adaptability to stress, in order to prevent stress damage caused by the effect. The effect of electrical stimulation and pre stimulation on the regulation of HRV in rats was observed by observing the changes of HRV and the changes of spectral components in rats after receiving electrical stimulation and pre stimulation.
Experimental method
40 healthy male Wistar rats, weighing 250 300g, provided by the experimental animal center of China Medical University. On the three day before the experiment the rats were placed in a room to avoid sound, light dark cycle (12h:12h - 8:00 - 20:00 back light), keep the room temperature 24 - 2 DEG C, humidity (55 + 5)%. Rats were reared in single cage, free food, water, were randomly divided into two groups in the experiment. Three days before the ECG let the rats adapt to 1 - 2 hours in the laboratory.
10 rats were randomly selected using needle electrode for 500 A current for 20 minutes, as the 500 A group. 10 rats were randomly selected using needle electrode for 1000 A current for 20 minutes, as the 1000 A group. 10 rats were randomly selected in the given 1000 A electrical stimulation before giving 50 A current for 20 minutes, as the pre stimulation group. 10 rats were randomly selected by the method of insertion of the electrode, but no current, as sham control group were measured respectively. Then ECG analysis, HRV analysis software to use the attached HRV, get the index data. The data with SPSS software. Daniel.
experimental result
500 A group compared with the sham operation group SDNN HRV time domain index (P < 0.05); RMSSD (P > 0.05) decreased; in the frequency domain index TP (P < 0.05) decreased; while LF/HF increased (P < 0.01).1000 A group compared with the sham operation group (SDNN HRV time domain index P < 0.05), RMSSD (P > 0.05) decreased; in the frequency domain index TP (P < 0.05) decreased; while LF/HF increased (P < 0.01). Pre stimulation group SDNN (P < 0.01), RMSSD (P < 0.05), TP (P < 0.05), LF/HF (P > 0.05) are increased, and LF/HF increased less than 1000 mu A stimulation group, suggesting that pre stimulation to weaken the electrical activity of sympathetic nerve stimulation caused by the elevation of the insular.
conclusion
1, the electrical stimulation of the island leaves decreased the SDNN and RMSSD in the HRV time domain of rats, and the frequency domain index TP decreased, but the LF/HF increased, and the change was reversible.
2, electrical stimulation of insular lobe can increase sympathetic nerve tension, decrease vagus nerve tension and decrease sympathetic nerve and vagus nerve dysfunction by acting on the autonomic nervous system. HRV can also decrease the function of sympathetic nerve and vagus nerve.
3, pre stimulation can reduce the effect of electrical stimulation on HRV and restore the HRV parameters to normal.

【學(xué)位授予單位】：中國醫(yī)科大學(xué)
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2008
【分類號】：R331.38

【參考文獻(xiàn)】

相關(guān)期刊論文 前5條

1 王藝明,劉興德,董為偉,楊宗城;電刺激大鼠島葉對心電圖和心率變異性的影響[J];中國醫(yī)師雜志;2003年12期

2 樓美清;陳先震;盧亦成;張光霽;;腦島及其相關(guān)結(jié)構(gòu)的臨床應(yīng)用解剖研究進(jìn)展[J];中華神經(jīng)外科疾病研究雜志;2005年06期

3 王藝明,劉興德,董為偉,楊宗城;電刺激大鼠島葉皮質(zhì)及皮質(zhì)下中樞對心臟自主神經(jīng)功能的影響[J];中國臨床康復(fù);2004年21期

4 莊亞純;心率變異性的研究現(xiàn)狀[J];心電學(xué)雜志;1994年04期

5 李長清,胡長林,董為偉;急性腦出血患者24小時(shí)心率變異性變化與動(dòng)態(tài)心電圖異常[J];中華心律失常學(xué)雜志;2002年03期

，

本文編號：1411278