發(fā)布時(shí)間：2018-01-10 12:15

  本文關(guān)鍵詞：鮑曼不動(dòng)桿菌耐藥分析及其intl1在生物被膜中作用機(jī)制探討　出處：《中南大學(xué)》2008年碩士論文　論文類型：學(xué)位論文

  更多相關(guān)文章： 鮑曼不動(dòng)桿菌 整合子 整合酶基因 生物被膜

【摘要】： 目的:了解長(zhǎng)沙地區(qū)鮑曼不動(dòng)桿菌的耐藥現(xiàn)狀,為臨床治療鮑曼不動(dòng)桿菌感染提供指導(dǎo);探討鮑曼不動(dòng)桿菌Ⅰ類整合子的分布以及Ⅰ類整合酶基因在生物被膜與液態(tài)培養(yǎng)下的表達(dá),為研究鮑曼不動(dòng)桿菌整合子在其生物被膜中的耐藥機(jī)制奠定基礎(chǔ)。 方法:①收集鮑曼不動(dòng)桿菌臨床菌株并進(jìn)行紙片擴(kuò)散法藥敏實(shí)驗(yàn)。②對(duì)收集的菌株進(jìn)行基因擴(kuò)增篩選出攜帶Ⅰ類整合酶的菌株,并檢測(cè)分析Ⅰ類整合子系統(tǒng)插入相關(guān)耐藥基因盒情況。③培養(yǎng)觀察形成生物被膜的菌株并對(duì)生物被膜形成能力進(jìn)行分析。④通過(guò)RT-PCR半定量方法分析Ⅰ類整合酶基因在生物被膜生長(zhǎng)狀態(tài)以及普通液相培養(yǎng)狀態(tài)下的表達(dá)情況。 結(jié)果:①Ⅰ類整合子相關(guān)基因盒陽(yáng)性菌株對(duì)8種抗菌藥物耐藥率依次為氨芐西林/舒巴坦56%、亞胺培南33%、美洛西林100%、妥布霉素100%、四環(huán)素100%、復(fù)方新諾明100%、環(huán)丙沙星100%和頭胞他啶100%,并且Ⅰ類整合子相關(guān)基因盒陽(yáng)性菌株對(duì)環(huán)丙沙星等7種抗生素耐藥率均顯著高于Ⅰ類整合子相關(guān)基因盒陰性菌株(經(jīng)X~2檢驗(yàn),差異有統(tǒng)計(jì)學(xué)意義,P＜0.05)。②64株臨床菌株攜帶Ⅰ類整合酶的有46株,相關(guān)基因盒陽(yáng)性的Ⅰ類整合子為9株。9株Ⅰ類整合子相關(guān)基因盒的擴(kuò)增產(chǎn)物集中在1500bp至3000bp之間。③46株鮑曼不動(dòng)桿菌中有42株能夠形成生物被膜。④生物被膜狀態(tài)下的鮑曼不動(dòng)桿菌Ⅰ類整合酶基因表達(dá)增高。在普通液態(tài)培養(yǎng)組一類整合酶基因表達(dá)量為0.973±0.754,而在生物被膜狀態(tài)組一類整合酶基因表達(dá)量則為2.545±0.674。 結(jié)論:臨床鮑曼不動(dòng)桿菌的耐藥情況嚴(yán)峻。Ⅰ類整合子分布廣泛,并且Ⅰ類整合子基因盒陽(yáng)性菌株耐藥率明顯高于Ⅰ類整合子基因盒陰性菌株。鮑曼不動(dòng)桿菌大多具有較強(qiáng)的生物被膜形成能力。Ⅰ類整合酶基因在不同生長(zhǎng)狀態(tài)下均可表達(dá),但在生物被膜生長(zhǎng)狀態(tài)下的表達(dá)增高。
[Abstract]:Objective: to understand the Changsha area Bauman real resistance of Bacillus, for the clinical treatment of Bauman still provide guidance on Bauman infection; Acinetobacter integron integrase gene and distribution in biological is the expression of membrane and liquid culture, for the study of Bauman Acinetobacter resistance integron was to lay the foundation in the film in its biological mechanism.
Methods: the collection of Bauman real clinical strains bacillus and disk diffusion susceptibility test. The strains were collected for gene amplification were screened with integrase strains, detection and analysis of class I integron insertion related resistance gene cassettes. To observe the formation of biofilm strains and the biological is film forming ability were analyzed. The cultured through the semi quantitative RT-PCR analysis method of integrase gene in the biofilm growth state and liquid state culture expression.
Results: 1 integron gene cassettes positive strains followed by ampicillin / sulbactam 56% to 8 kinds of antimicrobial drug resistance rate of imipenem 33%, mezlocillin 100%, 100% tobramycin, tetracycline 100%, cotrimoxazole 100%, ciprofloxacin 100% and ceftazidime 100%, and integron related gene cassette positive strains to ciprofloxacin and other 7 kinds of antibiotics were significantly higher than that of integron gene cassettes negative strains (by X~2 test, the difference was statistically significant, P < 0.05). The 64 strains carrying class 1 integrase are 46 strains, gene cassettes in integrons is positive PCR products of 9.9 strains of integron gene cassettes concentrated between 1500bp to 3000bp. The 46 strains of Bauman Acinetobacter in 42 strains could form biofilm. The biofilm state of the Bauman Acinetobacter integrase gene expression The expression of integrase gene is 0.973 + 0.754 in the ordinary liquid culture group, and the expression of an integrase gene is 2.545 + 0.674. in the biofilm state group.
Conclusion: the clinical Bauman real drug resistance of Bacillus grim. Integrons are widely distributed, and integron gene cassette positive strain resistance was significantly higher than that of integron gene cassette negative strains. Bauman Acinetobacter has strong biofilm forming ability. The class 1 integrase gene can be expressed in different the growth state, but in the biofilm growth under the condition of higher expression.

【學(xué)位授予單位】：中南大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2008
【分類號(hào)】：R378

【參考文獻(xiàn)】

相關(guān)期刊論文 前10條

1 顧兵;童明慶;趙文君;趙旺勝;張巧娣;;南京地區(qū)鮑曼不動(dòng)桿菌中整合子流行現(xiàn)狀及攜帶的耐藥基因分析[J];中國(guó)感染與化療雜志;2007年05期

2 ;Class Ⅰ integron with a novel cassette array in an ESBL-producing multidrug-resistant Klebsiella pneumoniae isolate[J];Journal of Nanjing Medical University;2006年01期

3 石磊;寇婭麗;;銅綠假單胞菌基因型與生物被膜形成能力的分析[J];食品與機(jī)械;2006年06期

4 戴春梅;鄭蘭香;陳輝;;129株鮑曼不動(dòng)桿菌所致醫(yī)院感染的耐藥性分析[J];實(shí)用預(yù)防醫(yī)學(xué);2006年01期

5 谷秀,李勝岐,張智潔,劉勇,李強(qiáng),潘莉莉;銅綠假單胞菌生物被膜中β-內(nèi)酰胺酶活性的檢測(cè)[J];中華內(nèi)科雜志;2001年09期

6 張宏梅,石磊,李琳,陳清,陳義忠,張喜梅;沙門(mén)菌中第一類整合子的鑒定及特性分析[J];中華微生物學(xué)和免疫學(xué)雜志;2004年03期

7 柴棟,王睿,裴斐,方翼,朱曼;銅綠假單孢菌生物被膜相關(guān)大鼠肺慢性感染免疫逃逸的研究[J];中國(guó)抗生素雜志;2003年09期

8 石磊;陳洵;肖增璜;;多重耐藥臨床菌株中整合子結(jié)構(gòu)的檢測(cè)與分析[J];中國(guó)抗生素雜志;2007年01期

9 嚴(yán)巖;李彤;劉樹(shù)林;;兩種培養(yǎng)基對(duì)銅綠假單胞菌和大腸埃希菌生物被膜厚度的影響[J];中國(guó)實(shí)驗(yàn)診斷學(xué);2007年05期

10 朱錦琪,羅百靈,胡成平,馮俊濤;1032株肺部感染致病菌分布及耐藥性分析[J];中國(guó)現(xiàn)代醫(yī)學(xué)雜志;2005年18期

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