本文關(guān)鍵詞：雙氫睪酮調(diào)節(jié)原代培養(yǎng)大鼠Sertoli細(xì)胞GDNF的表達(dá)　出處：《基礎(chǔ)醫(yī)學(xué)與臨床》2015年11期 　論文類型：期刊論文

  更多相關(guān)文章： 雙氫睪酮 Sertoli細(xì)胞 GDNF ERK 信號(hào)通路

【摘要】：目的觀察雙氫睪酮對(duì)原代培養(yǎng)大鼠Sertoli細(xì)胞GDNF及相關(guān)細(xì)胞信號(hào)通路關(guān)鍵蛋白的表達(dá),探討Sertoli細(xì)胞GDNF表達(dá)調(diào)控的機(jī)制。方法原代培養(yǎng)出生后20 d大鼠Sertoli細(xì)胞,給予雙氫睪酮干預(yù),免疫熒光檢測(cè)GDNF在細(xì)胞中定位和表達(dá)強(qiáng)度,Western blot檢測(cè)GDNF,ERK和CREB蛋白的表達(dá)及磷酸化情況,并用ERK和c AMP/PKA信號(hào)通路抑制劑干預(yù)。結(jié)果 GDNF表達(dá)在Sertoli細(xì)胞質(zhì)中,雙氫睪酮干預(yù)可增加GDNF的表達(dá)(P0.05),并使ERK和CREB磷酸化水平增高(P0.05),使用ERK和c AMP/PKA信號(hào)通路抑制劑可減低雙氫睪酮誘導(dǎo)的GDNF表達(dá)(P0.05)。結(jié)論雙氫睪酮通過(guò)激活ERK信號(hào)通路誘導(dǎo)Sertoli細(xì)胞GDNF的表達(dá)。
[Abstract]:Objective to investigate the effect of dihydrotestosterone on the expression of GDNF and related cell signal pathway key proteins in primary cultured rat Sertoli cells. To explore the mechanism of GDNF expression and regulation in Sertoli cells. Methods Sertoli cells were cultured 20 days after birth and treated with dihydrotestosterone. The localization and expression intensity of GDNF in the cells were detected by immunofluorescence. The expression and phosphorylation of GDNF ERK and CREB protein were detected by Western blot. ERK and c AMP/PKA signaling pathway inhibitors were used to intervene. Results GDNF was expressed in the cytoplasm of Sertoli. Dihydrotestosterone could increase the expression of GDNF and increase the phosphorylation level of ERK and CREB (P0.05). Using ERK and c AMP/PKA signaling pathway inhibitors can reduce GDNF expression induced by dihydrotestosterone (DHT). Conclusion dihydrotestosterone induces GDNF expression in Sertoli cells by activating ERK signaling pathway.
【作者單位】： 寧夏醫(yī)科大學(xué)基礎(chǔ)醫(yī)學(xué)院人體解剖與組織胚胎學(xué)系;寧夏醫(yī)科大學(xué)基礎(chǔ)醫(yī)學(xué)院生育力保持教育部重點(diǎn)實(shí)驗(yàn)室;寧夏醫(yī)科大學(xué)基礎(chǔ)醫(yī)學(xué)院寧夏生殖與遺傳重點(diǎn)實(shí)驗(yàn)室;
【基金】：國(guó)家自然科學(xué)基金(81160084) 寧夏醫(yī)科大學(xué)博士學(xué)位建設(shè)開(kāi)放課題(KF2010-15)
【分類號(hào)】：R321.1
【正文快照】： 在精子發(fā)生的有絲分裂階段,精原干細(xì)胞存在不平衡分裂,一方面通過(guò)自我更新形成干細(xì)胞儲(chǔ)備,一方面分化成為精原細(xì)胞。精原干細(xì)胞的自我更新是精子發(fā)生的持續(xù)進(jìn)行的保證�，F(xiàn)已發(fā)現(xiàn)數(shù)個(gè)調(diào)節(jié)精原干細(xì)胞(spermatogonial stem cells,SSCs)自我更新和分化的因子,如膠質(zhì)細(xì)胞源性神經(jīng)營(yíng)

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