本文關(guān)鍵詞：骨髓間充質(zhì)干細(xì)胞對白血病細(xì)胞生長影響的研究　出處：《南京醫(yī)科大學(xué)》2009年碩士論文　論文類型：學(xué)位論文

  更多相關(guān)文章： 間充質(zhì)干細(xì)胞 K562細(xì)胞 增殖 凋亡 小鼠 骨髓 間充質(zhì)干細(xì)胞 綠色熒光蛋白 白血病細(xì)胞

【摘要】：目的 比較K562細(xì)胞與相同數(shù)量以及不同數(shù)量的人骨髓間充質(zhì)干細(xì)胞(MSCs)粘附培養(yǎng)前后K562細(xì)胞增殖、細(xì)胞周期和凋亡的變化,以研究骨髓間充質(zhì)干細(xì)胞對人白血病K562細(xì)胞生長的影響。 方法 通過建立正常人骨髓間充質(zhì)干細(xì)胞的體外培養(yǎng)體系及其與K562細(xì)胞共培養(yǎng)的體系測定K562細(xì)胞的生長曲線;將不同數(shù)量的MSCs與K562細(xì)胞共培養(yǎng)測定K562細(xì)胞的增殖率曲線;流式細(xì)胞儀檢測K562細(xì)胞周期以及凋亡的變化。 結(jié)果 與單獨(dú)K562細(xì)胞培養(yǎng)相比,與相同數(shù)量MSCs共培養(yǎng)后,K562細(xì)胞生長受抑;與不同數(shù)量MSCs共培養(yǎng)后,其對K562細(xì)胞的抗增殖作用呈劑量依賴性;細(xì)胞周期分析發(fā)現(xiàn)K562細(xì)胞與MSCs共培養(yǎng)后,G0/G1期以及G2/M期的細(xì)胞增加, S期的細(xì)胞減少。共培養(yǎng)24 h、48 h、72 h后用流式細(xì)胞儀檢測其凋亡率,發(fā)現(xiàn)K562細(xì)胞的凋亡率下降。 結(jié)論 正常人骨髓間充質(zhì)干細(xì)胞能導(dǎo)致K562細(xì)胞生長抑制,阻止K562細(xì)胞周期的運(yùn)行,凋亡率下降,且在一定范圍內(nèi),隨著間充質(zhì)干細(xì)胞數(shù)量的增加其對K562細(xì)胞的抗增殖作用增強(qiáng)。 目的 觀察骨髓間充質(zhì)干細(xì)胞(BMMSC)對小鼠急性淋巴細(xì)胞白血病(ALL)模型中白血病細(xì)胞的作用; 方法 將攜帶綠色熒光蛋白(eGFP)基因標(biāo)記的P388細(xì)胞(小鼠急性淋巴細(xì)胞白血病細(xì)胞系)腹腔接種于DBA/2小鼠建立ALL小鼠動物模型,靜脈輸注體外培養(yǎng)的骨髓間充質(zhì)干細(xì)胞。記錄小鼠存活時間;監(jiān)測小鼠血常規(guī);流式細(xì)胞儀檢測組織器官標(biāo)本中的eGFP+細(xì)胞的百分比;觀察各小鼠臟器發(fā)生的病理學(xué)變化以及檢測輸注MSC的小鼠體內(nèi)Y染色體的存在。 結(jié)果 骨髓間充質(zhì)干細(xì)胞能延長ALL小鼠的生存時間,但其血常規(guī)變化不大; MSCs能夠減少各組織白血病細(xì)胞的浸潤,并具有向各組織定植的能力。 結(jié)論 骨髓間充質(zhì)干細(xì)胞能影響小鼠急性淋巴細(xì)胞白血病(ALL)模型中白血病細(xì)胞的生長。
[Abstract]:Purpose To compare the proliferation, cell cycle and apoptosis of K562 cells before and after adhesion culture with the same number and different numbers of human bone marrow mesenchymal stem cells (MSCs). To study the effect of bone marrow mesenchymal stem cells (BMSCs) on the growth of human leukemia K562 cells. Method The growth curve of normal human bone marrow mesenchymal stem cells (BMSCs) was determined by establishing the culture system in vitro and co-culture with K562 cells. The proliferation rate curve of K562 cells was measured by co-culture of different amounts of MSCs and K562 cells. The changes of K562 cell cycle and apoptosis were detected by flow cytometry. Results Compared with K562 cells alone, the growth of K562 cells was inhibited after co-culture with the same number of MSCs. The antiproliferative effect of MSCs on K562 cells was dose-dependent after co-culture with different amount of MSCs. Cell cycle analysis showed that K562 cells co-cultured with MSCs increased in G _ 0 / G _ 1 phase and G _ 2 / M phase, but decreased in S phase and co-cultured for 24 h or 48 h. After 72 hours, the apoptosis rate of K562 cells was detected by flow cytometry, and it was found that the apoptosis rate of K562 cells decreased. Conclusion Normal human bone marrow mesenchymal stem cells can induce K562 cell growth inhibition, prevent K562 cell cycle, apoptosis rate decreased, and within a certain range. As the number of mesenchymal stem cells increased, the anti-proliferation effect of mesenchymal stem cells on K562 cells was enhanced. Purpose To observe the effect of bone marrow mesenchymal stem cells (BMMSC) on leukemic cells in mice acute lymphoblastic leukemia (ALL) model. Method P388 cells (mouse acute lymphoblastic leukemia cell line) carrying green fluorescent protein (GFP) gene were inoculated intraperitoneally into DBA/2 mice to establish ALL mice model. Bone marrow mesenchymal stem cells (BMSCs) cultured in vitro were infused intravenously. Survival time of mice was recorded. The blood routine of mice was monitored. The percentage of eGFP cells in tissues and organs was detected by flow cytometry. The pathological changes of mouse organs and the existence of Y chromosome in mice injected with MSC were observed. Results Bone marrow mesenchymal stem cells (BMSCs) could prolong the survival time of ALL mice but had little change in blood routine. MSCs could reduce the infiltration of leukemic cells from various tissues and have the ability to colonize the tissues. Conclusion Bone marrow mesenchymal stem cells (BMSCs) can affect the growth of leukemic cells in acute lymphoblastic leukemia (ALL) model.
【學(xué)位授予單位】：南京醫(yī)科大學(xué)
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2009
【分類號】：R329

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