本文關(guān)鍵詞：切割穹窿海馬傘大鼠海馬內(nèi)BLBP的表達(dá)變化　出處：《神經(jīng)解剖學(xué)雜志》2008年06期 　論文類型：期刊論文

  更多相關(guān)文章： 穹窿海馬傘切割 腦脂結(jié)合蛋白 免疫印跡 免疫組織化學(xué) 海馬 大鼠

【摘要】：為了觀察切割大鼠右側(cè)穹窿海馬傘后,切割側(cè)與正常側(cè)海馬齒狀回內(nèi)腦脂結(jié)合蛋白(BLBP)的表達(dá)變化,本研究應(yīng)用Western blot和免疫組織化學(xué)方法檢測(cè)雙側(cè)海馬內(nèi)BLBP蛋白表達(dá)水平的變化,以及海馬齒狀回門(mén)區(qū)和顆粒下層中BLBP免疫陽(yáng)性細(xì)胞數(shù)和灰度值。結(jié)果顯示:正常大鼠雙側(cè)海馬各區(qū)和顆粒下層細(xì)胞BLBP僅有微量表達(dá),切割穹窿海馬傘后第1 d雙側(cè)差異不明顯;3 d時(shí)切割側(cè)顆粒下層陽(yáng)性細(xì)胞及染色深度較正常側(cè)加深;5 d時(shí)切割側(cè)顆粒下層陽(yáng)性細(xì)胞數(shù)量明顯增多,染色較深,并達(dá)到最高水平;7 d后BLBP免疫陽(yáng)性細(xì)胞的數(shù)量和染色深度開(kāi)始降低,14 d時(shí)接近正常側(cè)水平。而切割后3、5 d時(shí)雙側(cè)門(mén)區(qū)BLBP免疫陽(yáng)性細(xì)胞的數(shù)量差異不大,但切割側(cè)染色較深,7 d后也逐漸降低,14 d時(shí)降至正常側(cè)水平。上述結(jié)果提示,切割穹窿海馬傘阻斷了隔區(qū)與海馬齒狀回的纖維聯(lián)系后,可引起海馬齒狀回門(mén)區(qū)和顆粒下層BLBP表達(dá)增強(qiáng),可能引發(fā)了放射狀膠質(zhì)細(xì)胞的增殖和激活,構(gòu)成的支架有助于神經(jīng)干細(xì)胞的遷移和向神經(jīng)元的分化。
[Abstract]:In order to observe the changes of brain lipid binding protein (BLBP) in the dentate gyrus of the right fornix of the rat after cutting off the right fornix, the changes of the brain lipid binding protein (BLBP) in the dentate gyrus were observed. In this study, Western blot and immunohistochemistry were used to detect the expression of BLBP protein in bilateral hippocampus. As well as the number of BLBP immunoreactive cells and gray value in dentate gyrus and subgranular area. The results showed that the expression of BLBP in bilateral hippocampal regions and subgranular cells of normal rats was only slight. There was no significant difference between the two sides on the 1st day after fimbria fornix was cut. At 3 days, the positive cells and the staining depth of the subgranular subgranular in the incised side were deeper than those in the normal side. On the 5th day, the number of positive cells in the subgranular layer of the incised side increased obviously, the staining depth was deep, and reached the highest level. After 7 days, the number and staining depth of BLBP immunoreactive cells began to decrease. There was no significant difference in the number of BLBP immunoreactive cells in the bilateral portal area at the 5th day, but the staining on the incised side decreased gradually to the normal level at the 14th day after 7 days of deep staining. These results suggest that the number of BLBP immunoreactive cells decreased to the normal level at the 14th day. Cutting fornix hippocampal umbrella blocked the fibrous connection between the septum and dentate gyrus of the sea horse, which resulted in the increased expression of BLBP in the hilum of dentate gyrus and subgranular area of the sea horse, which may induce the proliferation and activation of radial glial cells. The scaffold is helpful for neural stem cell migration and neuronal differentiation.
【作者單位】： 南通大學(xué)醫(yī)學(xué)院人體解剖學(xué)教研室神經(jīng)生物學(xué)研究所江蘇省神經(jīng)再生重點(diǎn)實(shí)驗(yàn)室;
【基金】：南通大學(xué)校級(jí)科研基金(062075)資助項(xiàng)目
【分類號(hào)】：R361.2
【正文快照】： 本課題組近年的研究發(fā)現(xiàn),移植至切割穹窿海馬傘側(cè)海馬齒狀回中的神經(jīng)干細(xì)胞較移植于正常側(cè)中的更易于存活、遷移和向神經(jīng)元分化[1],提示切割穹窿海馬傘后,海馬中改變了的微環(huán)境有利于神經(jīng)干細(xì)胞存活、遷移和向神經(jīng)元分化。在海馬提取液的非變性聚丙烯酰胺凝膠電泳的研究中,我

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