本文關(guān)鍵詞：Ⅱ型膠原蛋白和降植烷大鼠關(guān)節(jié)炎兩種模型的建立與比較　出處：《廣西醫(yī)科大學(xué)》2008年碩士論文　論文類(lèi)型：學(xué)位論文

  更多相關(guān)文章： 類(lèi)風(fēng)濕關(guān)節(jié)炎 動(dòng)物模型 膠原誘導(dǎo) 降植烷誘導(dǎo)

【摘要】： 目的:本研究擬通過(guò)建立近交系Wistar大鼠的PIA和CIA兩種動(dòng)物模型,比較兩個(gè)模型的臨床特點(diǎn)、實(shí)驗(yàn)室檢查及病理特征的區(qū)別,為類(lèi)風(fēng)濕關(guān)節(jié)炎的基礎(chǔ)科學(xué)研究尋找更為理想的動(dòng)物模型。 方法:將60只大鼠隨機(jī)分為CIA組、PIA組、對(duì)照組,各20只,分別將Ⅱ型膠原0.01ml/g和降植烷0.003ml/g在各自組別的大鼠背部或尾根部進(jìn)行多點(diǎn)皮內(nèi)注射誘導(dǎo)建立PIA大鼠模型和CIA大鼠模型,對(duì)照組注射生理鹽水。觀察大鼠臨床特點(diǎn),采用關(guān)節(jié)評(píng)分、組織病理學(xué)觀察關(guān)節(jié)炎嚴(yán)重程度,判定模型建立是否成功,并比較兩組中成功建立模型的大鼠的病程特點(diǎn)、血清免疫學(xué)指標(biāo)(血清RF、抗CCP抗體)、病理特征和成模率。 結(jié)果:(1)CIA組和PIA組大鼠均出現(xiàn)關(guān)節(jié)炎癥狀,病理為以滑膜持續(xù)增生、大量炎性細(xì)胞浸潤(rùn)、軟骨及骨質(zhì)破壞為表現(xiàn)的侵蝕性滑膜炎改變。(2)CIA大鼠模型的成模率為65%,高于PIA大鼠模型的30%,差異有統(tǒng)計(jì)學(xué)意義(P＜0.05);(3)CIA大鼠關(guān)節(jié)腫脹出現(xiàn)時(shí)間較PIA早,腫脹程度比PIA嚴(yán)重,病理改變也比PIA模型重,并出現(xiàn)有軟骨的破壞。CIA大鼠的關(guān)節(jié)炎癥狀約在發(fā)病3周后出現(xiàn)緩解,后無(wú)嚴(yán)重的炎癥出現(xiàn),PIA大鼠的關(guān)節(jié)炎癥狀則反復(fù)出現(xiàn),不具自限性,較接近人類(lèi)RA的病程特點(diǎn);(4)PIA組檢測(cè)到部分血清RF呈低滴度升高,CIA組RF全部為陰性,兩組RF滴度均數(shù)差異有統(tǒng)計(jì)學(xué)意義,P＜0.05;兩組的抗CCP抗體均為陰性。 結(jié)論:(1)CIA模型和PIA模型均可以在近交系Wistar大鼠中成功建立; (2)在近交系Wistar大鼠中,CIA模型比PIA模型更實(shí)用。
[Abstract]:Objective: to establish a better animal model for rheumatoid arthritis by establishing two animal models of PIA and CIA in inbred Wistar rats, comparing the clinical characteristics, laboratory tests and pathological characteristics of the two models.
Methods: 60 rats were randomly divided into CIA group, PIA group and control group, 20 rats each, respectively, of type II collagen 0.01ml/g and pristane 0.003ml/g in their respective groups of rats back or tail root of model and CIA PIA rat model induced by multiple intradermal injection, control group injection to observe the clinical characteristics of saline. Rats with knee score, observed arthritis severity pathology, determine the model is successful, the clinical features were compared between the two groups in the model rats, serum immunological markers (serum RF, anti CCP antibody), pathological characteristics and incidence of diabetes.
緇撴灉:(1)CIA緇勫拰PIA緇勫ぇ榧犲潎鍑虹幇鍏寵妭鐐庣棁鐘，

本文編號(hào)：1369841