本文關(guān)鍵詞：豬帶絳蟲重組雙歧桿菌疫苗不同保存條件下的穩(wěn)定性研究　出處：《中國人獸共患病學(xué)報》2015年07期 　論文類型：期刊論文

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【摘要】：目的研究豬帶絳蟲TSO45W-4B基因、TSOL18基因和TSO45W-4B-TSOL18融合基因的重組雙歧桿菌(Bb)疫苗在不同保存條件下的穩(wěn)定性。方法分別將豬帶絳蟲重組質(zhì)粒pGEX-TSO45W-4B、pGEX-TSOL18和pGEX-TSO45W-4BTSOL18電轉(zhuǎn)入長雙歧桿菌(B.longum),獲得陽性菌株pGEX-TSO45W-4B/B.longum、pGEX-TSOL18/B.longum和pGEXTSO45W-4B-TSOL18/B.longum,分別置于不同條件下保存,取出菌液,抽提質(zhì)粒,電泳檢測,進(jìn)行穩(wěn)定性分析。結(jié)果陽性菌株pGEX-TSO45W-4B/B.longum、pGEX-TSOL18/B.longum和pGEX-TSO45W-4B-TSOL18/B.longum分別在常溫下、4℃、-20℃、-80℃、-196℃(液氮中)分別保存48h、1周、1月、2月,抽提質(zhì)粒,電泳發(fā)現(xiàn)陽性菌株pGEX-TSO45W-4B/B.longum、pGEX-TSOL18/B.longum和pGEX-TSO45W-4B-TSOL18/B.longum在液氮中、-80℃、-20℃中比較穩(wěn)定,而在普通的室溫環(huán)境下,很快發(fā)生質(zhì)粒丟失現(xiàn)象。結(jié)論豬帶絳蟲TSO45W-4B基因、TSOL18基因和TSO45W-4B-TSOL18融合基因的重組Bb疫苗低溫保存最穩(wěn)定,常溫最差,這為豬帶絳蟲重組Bb疫苗的進(jìn)一步研究奠定了基礎(chǔ)。
[Abstract]:Objective to study the TSO45W-4B gene of Taenia solium. Recombinant Bifidobacterium Bifidobacterium with TSOL18 Gene and TSO45W-4B-TSOL18 Fusion Gene. Methods Recombinant plasmid pGEX-TSO45W-4B of Taenia solium was prepared. PGEX-TSOL18 and pGEX-TSO45W-4BTSOL18 were electrotransferred to B.longum. The positive strain pGEX-TSO45W-4B/B.longum was obtained. PGEX-TSOL18/B.longum and pGEXTSO45W-4B-TSOL18 / B.longum were stored under different conditions, respectively. The plasmids were extracted, electrophoresis was performed and stability was analyzed. Results the positive strains were pGEX-TSO45W-4B/B.longum. PGEX-TSOL18/B.longum and pGEX-TSO45W-4B-TSOL18/B.longum were found to be at 4 鈩，

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