本文關(guān)鍵詞：慢病毒轉(zhuǎn)染兔骨髓基質(zhì)干細(xì)胞的研究　出處：《吉林大學(xué)》2010年碩士論文　論文類(lèi)型：學(xué)位論文

  更多相關(guān)文章： 骨髓基質(zhì)干細(xì)胞 慢病毒 轉(zhuǎn)染

【摘要】： 實(shí)驗(yàn)?zāi)康?探討骨髓基質(zhì)干細(xì)胞標(biāo)記失蹤的合理有效方法。 實(shí)驗(yàn)方法:以兔骨髓基質(zhì)干細(xì)胞(rabbit marrow stromal stem cells)為研究對(duì)象,抽取兔髂脊骨髓血,按貼壁細(xì)胞分離法分離骨髓基質(zhì)干細(xì)胞MSCs,進(jìn)行體外擴(kuò)增培養(yǎng)。用293FT細(xì)胞和PWPXLd、PAX2、PMD2.G三質(zhì)粒系統(tǒng)包裝攜帶綠色熒光蛋白(EGFP)基因的慢病毒(lentivirus),收獲慢病毒后,用病毒侵染兔骨髓基質(zhì)干細(xì)胞,在熒光顯微鏡下觀察轉(zhuǎn)染結(jié)果。 結(jié)果:分離培養(yǎng)出兔骨髓來(lái)源的MSCs,并用攜帶綠色熒光蛋白( EGFP)基因的慢病毒成功感染兔骨髓MSCs。 結(jié)論:獲得兔骨髓MSCs并進(jìn)行慢病毒載體介導(dǎo)的基因修飾,為基質(zhì)干細(xì)胞基因工程和組織工程研究奠定了基礎(chǔ)。
[Abstract]:Objective : To investigate the rational and effective method of marker disappearance of bone marrow stromal cells . Methods : The rabbit bone marrow stromal cells ( MSCs ) were isolated from rabbit bone marrow stromal cells . MSCs were isolated from bone marrow stromal cells by adherent cell separation method . The MSCs were isolated from bone marrow stromal cells by adherent cell separation method . The lentivirus ( lentivirus ) carrying green fluorescent protein ( EGFP ) gene was packed with 293FT cells and PWPXLd , PAX2 and PMD 2.G three plasmid systems . The rabbit bone marrow stromal cells were infected with virus , and the transfection results were observed under fluorescence microscope . Results : MSCs from rabbit bone marrow were isolated and cultured , and rabbit bone marrow MSCs were successfully infected with lentivirus carrying green fluorescent protein ( EGFP ) gene . Conclusion : To obtain rabbit bone marrow MSCs and carry out the gene modification mediated by the slow virus vector , a foundation is laid for the research of gene engineering and tissue engineering of stromal cells .

【學(xué)位授予單位】：吉林大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2010
【分類(lèi)號(hào)】：R373

【參考文獻(xiàn)】

相關(guān)期刊論文 前1條

1 李東,柳向東,肖開(kāi)顏,舒朝鋒,崔磊,劉偉,曹誼林;接種濃度對(duì)人骨髓基質(zhì)干細(xì)胞在脫鈣骨上生長(zhǎng)和分布的影響[J];上海第二醫(yī)科大學(xué)學(xué)報(bào);2004年04期

，

本文編號(hào)：1367148