本文關鍵詞：恐懼聲音心理應激對不同階段制小鼠學習記憶的影響機　出處：《陜西師范大學》2008年碩士論文　論文類型：學位論文

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【摘要】： 許多文獻報道,心理應激能夠損傷動物空間學習和記憶能力。而且隨著社會競爭、工作壓力以及家庭變異等社會應激源的逐步增加,心理因素相關疾病的發(fā)生率具有迅速增長的趨勢。因此,心理應激與學習記憶的關系以及對學習記憶能力的影響的研究也就成為生命科學領域的熱點。目前,國內關于研究心理應激與學習記憶的模型很多,如力竭游泳、束縛、熱應激以及電擊等,它們均存在軀體應激利心理應激兩種過程,難以評估單純心理應激的對學習記憶的影響效應;國外,對心理應激與學習記憶關系的研究也僅是處于慢性應激的階段。 本實驗通過建立的恐懼聲音心理應激模型,將實驗動物分為對照組、孕期組、幼年組以及青年組。觀察不同階段鼠齡小鼠的行為,畸形情況,并定期稱量各組小鼠的體重;采用Morris水迷宮檢測不同時期不同性別小鼠的空間學習記憶水平,以探測妊娠期、幼年期以及青年期小鼠接受應激刺激后對學習記憶能力的影響;通過熒光免疫組織化學方法觀察妊娠期、幼年期以及青年期接受刺激后各組小鼠海馬結構中N-甲基-D-天冬氨酸受體1(N-methyl-D-aspartic acidreceptor1,NMDAR1)的表達情況,探討應激影響對各組不同性別小鼠學習記憶能力的可能機制;高頻刺激(High frequency stimulation,HFS)被廣泛用來研究海馬CA1區(qū)長時程增強(Long-term potentiation,LTP)的誘導和維持的動力學特點,Schaffer側枝-CA1通路的LTP又是研究突觸可塑性的常用模型,本研究還通過高頻刺激,觀察孕期和幼年期小鼠的海馬處Schaffer側枝-CA1區(qū)LTP的誘發(fā)幅度。 行為觀察及體重稱量結果顯示,和對照組相比,恐懼聲音心理應激對不同時期雌性小鼠的行為有明顯的影響,表現為兩耳豎立、身體卷曲,將頭埋藏于刨花中;有打洞逃離的行為,不易捕捉,對人的恐懼水平提高,掙扎的程度增加;大小便頻繁、扎堆;恐懼聲音心理應激可抑制不同時期小鼠的體重的增加量,尤其是幼年聲音組表現尤為明顯,雄鼠的情況正好相反。另外,對小鼠出生率及成活率基本沒有影響。 Morris水迷宮實驗結果表明:空間搜索策略一恐懼聲音應激后,較對照組相比,孕期和幼年聲音組小鼠基本上沒有形成參考認知能力,青年聲音組基本上形成了參考認知能力,但較對照組差;學習能力一恐懼聲音心理應激能夠促進雌鼠的學習能力,但抑制雄鼠的學習能力;記憶能力—Morris水迷宮實驗結果表明,恐懼聲音心理應激對孕鼠的仔鼠記憶有促進作用,但是對幼年和青年時期的小鼠的記憶能力有抑制作用;另外,恐懼聲音對不同性別的小鼠的影響也不同,對于雌鼠,恐懼聲音心理應激抑制幼年期和青年期的雌鼠的記憶能力,促進孕鼠的雌性后代的記憶;對于雄鼠,恐懼聲音刺激對幼年期和青年期的雄鼠的空間記憶能力有一定的促進作用,對孕鼠的雄性后代有極大的抑制作用。 熒光免疫組化結果表明,恐懼聲音心理應激促進了NMDAR1亞單位在雌鼠海馬CA1區(qū)和CA3區(qū)的表達,抑制了其在雌鼠海馬齒狀回的表達;恐懼聲音心理應激抑制NMDAR1亞單位在雄鼠海馬CA1區(qū)、CA3區(qū)以及齒狀回的表達。 電生理實驗結果表明,恐懼聲音心理應激對海馬CA1區(qū)的LTP的形成和誘發(fā)有明顯的促進作用。 以上結果表明,本實驗成功建立了恐懼聲音心理應激模型�？謶致曇魬Σ煌瑫r期、不同性別小鼠的行為、體重及學習記憶能力均有不同影響。其影響的分子機制在于改變小鼠海馬CA1和CA3區(qū)NMDAR1亞單位的表達,并促進海馬CA1區(qū)的LTP形成和誘發(fā)。
[Abstract]:Many reports in the literature, psychological stress can damage animal spatial learning and memory ability. With the social competition, work stress and family variation and other social stressors gradually increase the diseases related to psychological factors have been growing rapidly. Therefore, research on psychological relationship between learning and memory and shock effect on the ability of learning and memory has become a hotspot in the field of life science. At present, many domestic research on psychological stress and learning and memory model, such as swimming, restraint, heat stress and electric shocks, are the psychological stress physical stresses and two kinds of process, it is difficult to assess the effect on learning and memory of psychological stress; abroad study on the relationship between psychological stress and learning and memory is only at the level of the chronic stress.
Through the experiments of terrified sound stress model, the experimental animal were divided into control group, pregnancy group, infant group and youth group. Rats were observed at different stages of mouse behavior, abnormal, and regularly weighed the mice body weight; using the Morris water maze test in different periods of different sex mice spatial learning and memory level, to detect pregnancy, infancy and young mice received stress stimulation effect on the ability of learning and memory; by fluorescence immunohistochemistry method to observe the pregnancy, infancy and adolescence when stimulated mice in hippocampus of N- methyl -D- aspartate receptor 1 (N-methyl-D-aspartic, acidreceptor1, NMDAR1) expression. To explore the possible mechanism of stress effects on learning and memory ability of each group of different sex mice; high frequency stimulation (High frequency, stimulation, HFS) is widely used to study Hippocampal CA1 long-term potentiation (Long-term, potentiation, LTP) in the induction and maintenance of the dynamic characteristics of Schaffer, LTP and -CA1 pathway collateral is a common model for the study of synaptic plasticity, through the high-frequency stimulation evoked amplitude observed during pregnancy and infancy mice hippocampus -CA1 area LTP Schaffer collateral.
Behavior observation and weighing results show that compared with the control group, there is obvious influence behavior of terrified sound stress in different periods of female mice, showed two erect ears, body curled, head buried in a hole in the wood; escape behavior, is not easy to catch, the fear level, struggle the degree of increase; the toilet frequently get together; terrified sound stress can inhibit the weight of mice in different periods increase, especially the youth voice group is obvious in male rats, the opposite is true. In addition, there were no influence on the birthrate and survival rate.
Morris water maze test results show that the space search strategies of terrified sound stress, compared with the control group, the pregnancy and juvenile mice basically did not form a sound reference to cognitive ability, youth voice group basically formed a reference to cognitive ability, but worse than that of control group; psychological stress can promote the learning ability of a sound of fear of female rats learning ability, but the inhibition of male rats, learning ability and memory ability; the experimental results showed that the Morris water maze, rats memory terrified sound stress of pregnant rats was promoted, but has inhibitory effect on youth and youth in the small memory capacity; in addition, affect the sound of fear of different sex mice also different, for female rats, terrified sound stress inhibition of childhood and adolescent female rats memory ability, promote the female offspring of pregnant rats memory; for males, terrified sound stimulation on the young The spatial memory ability of male rats in the year and adolescence has a certain promotion effect, which has a great inhibitory effect on the male offspring of pregnant rats.
Fluorescence immunohistochemistry results showed that the terrified sound stress promoted the expression of NMDAR1 subunit in the hippocampus CA1 area and CA3 area of female rats, inhibit its expression in the dentate gyrus of female rats; terrified sound stress inhibition of NMDAR1 subunit in hippocampus CA1 area of male rats, CA3 region and dentate gyrus of expression.
The results of electrophysiological experiments showed that the psychological stress of fear sounds significantly promoted the formation and induction of LTP in the hippocampal CA1 region.
The above results show that the experiment successfully established the model of terrified sound stress. In different periods of terrified sound stress, mice of different sex behavior, the influence of different weight and the ability of learning and memory. The molecular mechanism of the effect is that the expression change of mice hippocampal CA1 and CA3 regions of NMDAR1 subunit, and promote the formation of LTP in hippocampal CA1 region and induced.

【學位授予單位】：陜西師范大學
【學位級別】：碩士
【學位授予年份】：2008
【分類號】：R395

【引證文獻】

相關博士學位論文 前1條

1 馮婷;四逆散加味對睡眠剝奪大鼠學習記憶影響的分子生物學機制研究[D];北京中醫(yī)藥大學;2012年

相關碩士學位論文 前1條

1 劉佳佳;大鼠恐懼應激模型中行為與心血管指標變化[D];第二軍醫(yī)大學;2011年

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本文編號：1364225