本文關(guān)鍵詞：不同類茶對(duì)高脂模型大鼠減肥和防治非酒精性脂肪肝作用的研究　出處：《云南農(nóng)業(yè)大學(xué)》2013年碩士論文　論文類型：學(xué)位論文

  更多相關(guān)文章： 不同茶葉 高脂模型大鼠 減肥 非酒精性脂肪肝

【摘要】：現(xiàn)代科學(xué)研究證明茶葉具有多種保健功效，其中包括降血脂、降血糖、抗氧化、防輻射等，，但不同的茶葉對(duì)減肥和預(yù)防非酒精性脂肪肝的比較研究鮮見報(bào)道。據(jù)此，本研究以普洱茶（熟茶）、紅茶、烏龍茶（鐵觀音）、普洱茶（熟茶）茶粉、黑茶茶粉和六堡茶為試驗(yàn)材料，以降脂藥物為對(duì)照，利用外源性膳食誘導(dǎo)引起實(shí)驗(yàn)動(dòng)物高脂血癥及形成非酒精性脂肪肝動(dòng)物模型之后，采用不同劑量的茶湯對(duì)其進(jìn)行持續(xù)30天的灌胃，測(cè)定實(shí)驗(yàn)大鼠相關(guān)肝功能和血清生理生化指標(biāo)，并進(jìn)行肝臟組織和腹脂肪病理檢測(cè)，此外通過分子水平研究，觀察靶器官P4502E1和IGF-ImRNA基因的表達(dá)情況，判定肝組織病變程度，通過現(xiàn)代醫(yī)學(xué)方法和分子生物學(xué)手段分析比較受試茶樣對(duì)高脂模型大鼠減肥和預(yù)防非酒精性脂肪肝的作用，為其保健功效的評(píng)價(jià)提供基礎(chǔ)依據(jù)。 研究結(jié)果如下： 1、減肥作用 （1）普洱茶（熟茶）、鐵觀音、紅茶、普洱茶（熟茶）茶粉、黑茶茶粉、六堡茶均可有效抑制大鼠體重的增長(zhǎng)。 （2）（低、中劑量）普洱茶（熟茶）、（低、高劑量）鐵觀音、（低、高劑量）紅茶、中、低劑量）六堡茶可顯著提升胰脂肪酶活性（P＜0.01）。 （3）普洱茶（熟茶）、鐵觀音、紅茶、（高劑量）黑茶茶粉和（高劑量）六堡茶促進(jìn)腹脂肪重量顯著降低（P＜0.01） （4）通過對(duì)脂肪細(xì)胞大小測(cè)量以及細(xì)胞組織顯微鏡觀察結(jié)果顯示，普洱茶（熟茶）、鐵觀音、紅茶、（中劑量）普洱茶（熟茶）茶粉、（高劑量）、黑茶茶粉（高劑量）六堡茶均可有效抑制肥胖大鼠腹部脂肪細(xì)胞的增大，減少大鼠腹部脂肪的過多累積。 2、預(yù)防非酒精性脂肪肝 （1）通過對(duì)大鼠血清中ALT（谷丙轉(zhuǎn)氨酶）、AST（谷草轉(zhuǎn)氨酶）測(cè)定，得出普洱茶（熟茶）、（低、中劑量）鐵觀音和（低劑量）紅茶、普洱茶（熟茶）茶粉、六堡茶、黑茶茶粉可使試驗(yàn)大鼠血清中的AST水平顯著降低（P＜0.05，P＜0.01）；普洱茶（熟茶）和(低、高劑量)紅茶、(低劑量)普洱茶（熟茶）茶粉可使試驗(yàn)大鼠血清中的ALT水平顯著降低（P＜0.05，P＜0.01）。 （2）通過對(duì)大鼠肝勻漿中MDA(丙二醛)、SOD(超氧化物歧化酶)和GSH-Px（谷胱甘肽過氧化物酶）活性測(cè)定得出，普洱茶（熟茶）茶粉、黑茶茶粉和六堡茶均可有效改善大鼠肝勻漿組織的氧化損傷。 （3）通過對(duì)大鼠肝臟病理組織學(xué)觀察以及病變?cè)u(píng)分顯示:灌胃不同劑量的普洱茶（熟茶）、六堡茶能夠梯度性的改善由高脂飼料引起的肝臟脂肪變性。 3、分子水平研究 （1）與高脂模型組相比，普洱茶（熟茶）、紅茶、普洱茶（熟茶）茶粉、各高劑量六堡茶可使大鼠肝臟細(xì)胞中P4502E1表達(dá)量降低，其中（高劑量）普洱茶（熟茶）、（高劑量）紅茶最為顯著（P＜0.05）。 （2）與高脂模型組相比，普洱茶（熟茶）（高劑量）、鐵觀音、普洱茶（熟茶）茶粉（高劑量）、黑茶茶粉（中劑量）、六堡茶（中、高劑量）可使肝細(xì)胞中IGF-I基因表達(dá)量顯著升高（P＜0.05），其中普洱茶（熟茶）、六堡茶效果最好。 結(jié)論：普洱茶（熟茶）、鐵觀音、紅茶、普洱茶（熟茶）茶粉、黑茶茶粉、六堡茶對(duì)減肥、非酒精性脂肪肝都具有不同程度的保護(hù)作用，但普洱茶（熟茶）、六堡茶的作用更顯著。
[Abstract]:Modern scientific research shows that tea has many health benefits, including reducing blood fat, lowering blood sugar, antioxidant, anti radiation, but different tea to lose weight and prevent nonalcoholic fatty liver disease comparative study is rarely reported. Therefore, in this study, Pu'er Tea (tea), red tea, Pu'er, Oolong Tea (Tie Guanyin) tea (tea) tea powder, tea tea powder and tea as experimental material, taking lipid-lowering drugs as control, after the use of exogenous dietary induced animal experimental hyperlipidemia and formation of nonalcoholic fatty liver disease animal model, using different doses of tea for 30 days of intragastric administration on the determination of the the rat liver function and serum biochemical indexes, and pathological examination of liver tissue and abdominal fat, the molecular level study, observe expression of target organ of P4502E1 and IGF-ImRNA genes, determining the degree of liver tissue lesions, through modern The effects of tea samples on weight loss and prevention of non-alcoholic fatty liver disease in high-fat model rats were analyzed and compared by medical methods and molecular biological methods.
The results of the study are as follows:
1, the effect of weight loss
(1) Pu'er Tea (tea), tea, Black Tea, Pu'er Tea (tea) tea powder, tea tea, liupao tea could effectively inhibit the growth of body weight of rats.
(2) (low dose) Pu'er Tea (tea), (low, high dose) Tieguanyin, (low, high dose, low dose) Black Tea, Liupu tea) can significantly enhance the activity of pancreatic lipase (P < 0.01).
(3) Pu'er Tea (tea), tea, Black Tea (Gao Jiliang), black tea and tea powder (high dose) of Liupu tea promote abdominal fat weight significantly decreased (P < 0.01)
(4) through the display of fat cell size and cell and tissue measuring microscope observation results, Pu'er Tea (tea), tea, Black Tea, (middle dose) Pu'er Tea (tea) tea powder, tea powder, tea (Gao Jiliang) (Gao Jiliang) increase of Liupu tea could effectively inhibit the fat rat abdominal fat cells. To reduce abdominal fat in rats with excessive accumulation.
2, prevention of nonalcoholic fatty liver
(1) the rat serum ALT (alanine aminotransferase), AST (aspartate aminotransferase) were obtained Pu'er Tea (tea), (low dose) and Tieguanyin (low dose) Black Tea Pu'er Tea (tea), tea powder, tea, black tea powder can make the blood test in the level of AST was significantly decreased (P < 0.05, P < 0.01); Pu'er Tea (tea) and (low, high dose) Black Tea, (low dose) Pu'er Tea (tea) tea powder can make the serum test in rats of ALT were significantly decreased (P < 0.05, P < 0.01).
(2) the rat liver homogenate MDA (MDA), SOD (superoxide dismutase) and GSH-Px (glutathione peroxidase) activity determined that Pu'er Tea (tea), tea powder, tea tea powder and tea can effectively improve the oxidative injury of liver homogenate tissue of rats.
(3) the score of rat liver lesions and histopathological observation showed that intragastric administration of different doses of Pu'er Tea (tea), can improve the gradient of Liupu tea caused by high fat diet fatty degeneration of the liver.
3. Molecular level studies
(1) compared with model group, Pu'er Tea (tea), Black Tea, Pu'er Tea (tea) tea powder, the high dose of Liupu tea can be decreased P4502E1 expression in liver cells of rats, which (high dose) Pu'er Tea (tea), (Gao Jiliang) was the most significant (P < Black Tea 0.05).
(2) compared with model group, Pu'er Tea (tea), tea (Gao Jiliang), Pu'er Tea (tea) tea powder (Gao Jiliang), black tea powder (middle dose), Liupu tea (in high doses) can make the IGF-I gene expression in liver cells increased significantly (P < 0.05) among them, Pu'er Tea (tea), the best effect of liupao tea.
Conclusion: Pu'er Tea (tea), tea, Black Tea, Pu'er Tea (tea) tea powder, tea tea, liupao tea to lose weight, all have different degrees of protection of nonalcoholic fatty liver disease, but Pu'er Tea (tea), significantly more Liupu tea role.

【學(xué)位授予單位】：云南農(nóng)業(yè)大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2013
【分類號(hào)】：R285.5;R-332

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