本文關鍵詞：不同類型的應激對血清實驗指標的影響及其與HLA-DQB1等位基因的關聯(lián)　出處：《大連醫(yī)科大學》2008年碩士論文　論文類型：學位論文

  更多相關文章： 考試應激 刑事拘留 睡眠剝奪 實驗指標 HLA-DQB1

【摘要】： 心理應激是由于個體在生活適應過程中,因環(huán)境要求與自身應付能力不平衡的認識所引起的一種緊張狀態(tài)。應激主要由心理神經(jīng)內分泌和免疫系統(tǒng)共同參與,通過下丘腦-垂體-腎上腺軸(HPA)和交感神經(jīng)系統(tǒng)(SNS)調節(jié)免疫功能。機體免疫功能的紊亂造成機體免疫監(jiān)視和免疫清除功能下降,使機體易發(fā)感染、自身免疫病和腫瘤等疾病。應激的研究目的是為人類的健康服務,選取人作為研究對象,采用天然的應激原使實驗結果真實可信。因此選取不同類型的應激人群,檢測其與正常對照人群的血清免疫、生化、凝血指標的變化,并結合其與HLA-DQB1等位基因的關系;動物模型的資源相對易得,可操作性強,因此建立束縛應激和熱應激兩種應激動物模型,檢測其凝血功能的變化。共同探討應激對機體的免疫功能的影響和其相關機制。 方法 1.隨機選取沈陽某重點中學(畢業(yè)班)學生27人,男生15人,女生12人,年齡18～20歲,高考前1個月采集血樣,作為實驗組;隨機挑選某學校(入學新生)學生24人,男生12人,女生12人,年齡18～22歲,開學2個月后采集血樣,作為對照組。 1.1研究考試應激對機體心理狀態(tài)的影響,采用狀態(tài)-特質焦慮量表和壓力自評量表評估考試應激狀態(tài)下機體的心理狀態(tài),評價應激狀態(tài)下機體的心理反應。 1.2分別檢測考試應激和對照組的血清生化、免疫指標,探討應激對機體血液成分的影響。 2.隨機挑選某地刑事拘留人員18人,(年齡43.28±11.04歲),男、女各半,刑拘7-15天后采血。對照組是隨機挑選正常生活在社會生活中的群體成員24人,(年齡22.79±2.8歲),男、女各半。 2.1檢測刑事拘留應激人員和對照組的血生化、免疫指標,探討應激對機體血液成分的影響。 2.2檢測刑事拘留應激人員和對照組的凝血指標,探討應激對機體凝血系統(tǒng)的影響。 3.隨機挑選大連醫(yī)科大學本科、研究生10名,(年齡22-28歲),男、女各半,第一天正常睡眠,第二天晚上睡眠剝奪。 3.1分別檢測正常睡眠后和睡眠剝奪后的血生化、免疫指標探討應激對機體血液成分的影響。 3.2分別檢測正常睡眠后和睡眠剝奪后的凝血指標,探討應激對機體凝血系統(tǒng)的影響。 4.健康普通級昆明系(KM)小鼠,雌雄兼用,體重20-25g,應用束縛和熱應激建立了兩種小鼠應激動物模型和正常對照組。4.1分別檢測三組小鼠的凝血指標,探討應激對動物凝血系統(tǒng)的影響。 5.不同類型應激原所致的血清生化、免疫、凝血指標變化的比較。 6.采用PCR-SSP技術,建立HLA-DQB1等位基因的檢測方法。對大學生HLA-DQB1等位基因頻率進行檢測。 7.綜合得到的應激對機體生物學指標的影響及其與HLA-DQB1等位基因的關系,從基因角度探討應激對機體的影響。 結果 1.考試應激 1.1采用狀態(tài)特質-焦慮問卷(STAI)和壓力自評量表評估應激狀態(tài)下機體的心理狀態(tài)。確認高考前一個月的學生在焦慮狀態(tài)和焦慮特質的得分均顯著的高于全國的常模。而壓力評分與常模無明顯的差異。 1.2應考期與一般學習期(對照組)血清生化指標比較白蛋白(Alb)、白球比(a/g)、葡萄糖(Glu)、堿性磷酸酶(Alp)、鉀(K)實驗組較對照組顯著升高。谷草轉氨酶(AST)在應考期較一般學習期明顯降低。應考期與一般學習期(對照組)血清免疫指標比較C3在應考期較一般學習期明顯升高。 2.刑事拘留 2.1亞急性心理應激對刑事拘留人員會造成葡萄糖、鉀離子、總膽固醇、高密度脂蛋白、低密度脂蛋白的顯著升高;胰腺功能以及脂類可能是應激的敏感因子。 2.2亞急性心理應激對刑事拘留人員使IgA,C3,C4顯著升高。 2.3亞急性應激對刑事拘留人員凝血指標的檢測使凝血酶原形成時間縮短,而凝血酶時間延長。 3.睡眠剝奪 3.1睡眠剝奪使血清肌酐,總膽紅素,直接膽紅素顯著降低,總蛋白顯著升高。 3.2睡眠剝奪使凝血指標PT顯著性降低。 4.應激組小鼠PT、APTT、TT值都較正常組延長,而兩組不同應激的小鼠測定值無統(tǒng)計學顯著差異。 5.高考應激和刑事拘留應激共同使葡萄糖、鉀離子和補體C3水平升高;高考應激和睡眠剝奪應激共同使直接膽紅素水平降低;刑事拘留應激和睡眠剝奪應激共同使PT水平下降。束縛應激和熱應激在凝血指標上無明顯差異。6.HLA-DQB1等位基因頻率由高到低依次是:*03,*06,*05,*02,*04。 7.血清生化指標與HLA-DQB1等位基因的關聯(lián)HLA-DQB1各等位基因陽性攜帶和陰性攜帶者各檢測指標水平差異均無統(tǒng)計學意義。 結論 1.高考,刑事拘留,睡眠剝奪均可以作為應激原而引起心理方面的改變,本文的研究人群可以作為應激的研究對象。 2.采用的束縛應激和熱應激這兩種應激是有效的應激動物模型。 3.不同類型的應激造成相應的生化、免疫、凝血指標的升高或降低有所不同。結果提示生化指標可以較客觀的反應各器官的功能和軀體的健康程度。葡萄糖、鉀離子、直接膽紅素、補體C3、PT可為心理應激敏感因子。建立的小鼠心理應激和軀體應激的模型中,凝血因子不是軀體應激的相關性因子。APTT和TT是非特異性應激指標。
[Abstract]:Psychological stress is due to the individual life in the process of adaptation, because of a nervous state and their cognitive ability to cope with environmental requirements imbalance caused by psychological stress. Neuroendocrine and immune system, the hypothalamic pituitary adrenal axis (HPA) and sympathetic nervous system (SNS) to regulate the immune function. Immune dysfunction caused by immune surveillance and immune function decline, so that the body is prone to infection, autoimmune diseases and cancer and other diseases. The purpose of the study is to stress the human health services, selected as the research object, using natural stressor. The experiment result is true and credible. So choose different types of stressors the detection, determination of serum and normal controls biochemical changes of coagulation index, and its relationship with the HLA-DQB1 allele; animal model of resources that are easy to get, but Therefore, two stress animal models of restraint stress and heat stress were established to detect the changes of coagulation function, and to explore the influence of stress on the immune function of the body and its related mechanisms.
Method
1. randomly selected from a key middle school of Shenyang (graduating class) 27 students, 15 boys and 12 girls, age 18~20 years, 1 months before the college entrance examination blood samples were collected as experimental group; randomly selected a school (freshmen) 24 students, 12 boys and 12 girls, age 18~22 years, the school 2 months after the blood samples were collected as the control group.
1.1, we studied the influence of examination stress on the mental state of the body. We used state trait anxiety scale and stress self rating scale to assess the mental state of the body under the stress condition of examination, and evaluated the psychological response of the body under stress.
1.2 test the serum biochemical and immune indexes of the test stress and the control group, and discuss the effect of stress on the blood components of the body.
2., a total of 18 criminal detainees were selected at random (age 43.28 + 11.04 years). Male and female were half and arrested for 7-15 days. Blood samples were collected from the control group. The control group was randomly selected from 24 members of the group living in social life (age 22.79 to 2.8 years), male and female half.
2.1 the blood biochemical and immune indexes of the criminal detention stress personnel and the control group were detected, and the influence of stress on the blood components of the body was discussed.
2.2 the blood coagulation indexes of the stress personnel and the control group were detected and the effect of stress on the body's blood coagulation system was investigated.
3. randomly selected Dalian Medical University undergraduate, 10 graduate students (age 22-28 years old), male, female half, the first day normal sleep, second nights sleep deprivation.
3.1 the blood biochemistry after normal sleep and sleep deprivation was detected respectively, and the effects of stress on the blood components of the body were investigated.
3.2 the indexes of blood coagulation after normal sleep and sleep deprivation were detected respectively, and the effect of stress on the body's coagulation system was investigated.
4. healthy normal Kunming line (KM) mice, both male and female, weighing 20-25g. Two kinds of mouse stress animal models and normal control group were established by restraint and heat stress..4.1 was used to detect coagulation indexes of three groups of mice, and to explore the effect of stress on animal coagulation system.
5. the changes of serum biochemical, immune and coagulation indexes caused by different types of stress.
6. the detection method of HLA-DQB1 allele was established by PCR-SSP technique. The frequency of HLA-DQB1 allele in college students was detected.
7. the effects of stress on the biological indicators of the body and the relationship with the HLA-DQB1 allele, and the effect of stress on the body from the perspective of gene.
Result
1. test stress
1.1 using the State Trait Anxiety Inventory (STAI) and self rating scale to assess the psychological status of the body under stress. Confirm one month before the college entrance examination students in state anxiety and trait anxiety scores were significantly higher than the national norm. The stress score and the norm has no obvious difference.
1.2 exam and general learning period (control group) serum biochemical indicators of albumin (Alb), than the white ball (a/g), glucose (Glu), alkaline phosphatase (Alp), potassium (K) experimental group was significantly higher than that of control group (AST). AST in the exam than the general learning stage decreased should the exam. And general learning period (control group) serum immune index C3 in the exam should be more general learning period increased significantly.
2. criminal detention
2.1, subacute psychological stress can cause significant increases in glucose, potassium, total cholesterol, high-density lipoprotein and low density lipoprotein in criminal detainees, and pancreatic function and lipids may be sensitive factors of stress.
2.2 subacute psychological stress significantly increased IgA, C3, and C4 for criminal detainees.
2.3 the detection of coagulation indexes in criminal detainees caused by subacute stress shortened the time of prothrombin formation and the time of thrombin prolonged.
3. sleep deprivation
3.1 sleep deprivation significantly decreased serum creatinine, total bilirubin and direct bilirubin, and increased the total protein.
3.2 sleep deprivation reduced the significance of coagulation index PT.
The values of PT, APTT and TT in the 4. stress group were all longer than those in the normal group, but there was no significant difference between the two groups of mice with different stress.
5. college entrance examination stress and stress of criminal detention to make glucose, potassium and elevated levels of complement C3; college entrance examination stress and sleep deprivation stress reduced the direct bilirubin level of the common criminal detention; stress and sleep deprivation stress decreased the level of PT in common. Restraint stress and heat stress on blood coagulation index did not differ significantly between the.6.HLA-DQB1 allele frequency is high to low is: *03, *06, *05, *02, *04.
7. there was no significant difference in serum biochemical indexes and HLA-DQB1 alleles between HLA-DQB1 alleles positive carriers and negative carriers.
conclusion
1. the college entrance examination, criminal detention and sleep deprivation can all cause psychological changes as the source of stress. The research population in this paper can be used as the research object of stress.
2. the two kinds of stress, the restraint stress and the heat stress, are the effective animal models of stress.
3. different types of stress caused by the biochemical, immune, blood coagulation index increased or decreased in different degree. The results suggest that health biochemical indexes of each organ can be more objective response function and body. Glucose, potassium ion, direct bilirubin, complement C3, PT can be a sensitive factor. The mice heart should be stimulated and psychological stress the physical stress model, coagulation factor is not physical stress correlation factor.APTT and TT are non specificity index.

【學位授予單位】：大連醫(yī)科大學
【學位級別】：碩士
【學位授予年份】：2008
【分類號】：R392

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