【摘要】：目的探討Notch1信號通路在骨髓間充質(zhì)干細(xì)胞(MSCs)移植后促進(jìn)腦缺血區(qū)血管新生過程中的作用。方法分離、培養(yǎng)SD大鼠MSCs,制作大鼠大腦中動脈栓塞(MCAO)模型,將試驗(yàn)分為正常組、腦缺血后MSCs移植組、腦缺血后MSCs移植+rh NF-κB組、腦缺血對照組。腦組織冰凍切片行Ⅷ因子免疫組織熒光染色檢測各試驗(yàn)組MSCs移植1、4、7、14 d后缺血皮質(zhì)區(qū)微血管數(shù)量。Western印跡檢測各試驗(yàn)組MSCs移植1、4、7、14 d后缺血皮質(zhì)區(qū)血管內(nèi)皮生長因子(VEGF)165、Notch1、Hes1蛋白表達(dá)。結(jié)果缺血對照組、MSCs移植組和MSCs移植+rh NF-κB組在各時(shí)間點(diǎn)微血管數(shù)量及VEGF165、Notch1、Hes1蛋白表達(dá)均顯著高于正常組,從第14天起開始下降,3組的微血管數(shù)量及VEGF165、Notch1、Hes1蛋白表達(dá)依次上升,差異顯著(P0.01)。結(jié)論 MSCs移植可促進(jìn)缺血區(qū)新生血管的形成,并可通過激活Notch信號通路促進(jìn)缺血皮質(zhì)區(qū)VEGF165的表達(dá),從而促進(jìn)缺血區(qū)的血管新生。
[Abstract]:Objective to investigate the role of Notch1 signaling pathway in promoting angiogenesis after (MSCs) transplantation of bone marrow mesenchymal stem cells (BMSCs). Methods the (MCAO) model of middle cerebral artery embolism (MCAE) was established in SD rats by MSCs, culture. The rats were divided into normal group, MSCs transplantation group, rh NF- 魏 B transplantation group and cerebral ischemia control group. The number of microvessels in ischemic cortex was detected by immunohistochemical staining of factor 鈪，

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