河北某地區(qū)ORP9基因多態(tài)性與腦梗死的相關(guān)性研究
發(fā)布時間：2019-01-01 13:35
【摘要】：目的將氧化固醇相關(guān)蛋白-9(Oxysterol·Binding Protein-related Protein-9,ORP-9)作為腦梗死的候選基因,檢測其在河北漢族人群中的分布頻率,分析ORP-9的基因多態(tài)性與腦梗死的關(guān)系,并分析其與腦梗死的關(guān)鍵性危險因素-動脈粥樣硬化(Atheroselerosis,AS)、血脂、血壓及血糖的關(guān)系。方法選華北理工大學(xué)附屬醫(yī)院收治的籍貫來自河北地區(qū)無血緣關(guān)系的腦梗死患者174人作為腦梗死組,另選年齡和性別相仿的河北籍貫的無血緣關(guān)系的來自同一醫(yī)院無腦梗死患者或者同期到華北理工大學(xué)附屬醫(yī)院體檢中心的體檢健康者作為對照組,共117例。于清晨抽取研究對象的血液樣本5ml,并追蹤其病史及相關(guān)檢驗、檢查,與對照組進(jìn)行病例對照研究。把抽的血液樣本使用全血提取試劑盒提取DNA,然后做PCR檢測,把經(jīng)過PCR擴增的產(chǎn)物進(jìn)行限制性片段長度多態(tài)性分析(Restriction Fragment Length Polymorphism,RFLP),用來檢測ORP-9的兩個位點rs856600及rs768529的單核苷酸多態(tài)性,檢測其基因型及等位基因的分布。使用t檢驗、卡方檢驗及Logistic回歸模型處理統(tǒng)計數(shù)據(jù),并且使用SPSS l 7.0統(tǒng)計軟件對得到的統(tǒng)計數(shù)據(jù)進(jìn)行分析。結(jié)果1在腦梗死組與對照組的基本臨床資料的比較中,在很多方面,如年齡、性別、吸煙史及飲酒史上,兩組間無統(tǒng)計學(xué)意義(P0.05);與對照組相比,腦梗死組在體重指數(shù)、糖尿病史、高血壓病史、收縮壓、舒張壓、空腹血糖、TG、TC及LDL比例大(P0.05);而腦梗死的HDL比對照組的人數(shù)少(P0.05)。2rs856600與rs768529的基因型與等位基因頻率在腦梗死與對照組比較中均無統(tǒng)計學(xué)意義(P0.05);它們的Logistic回歸分析結(jié)果,所有基因型及等位基因頻率和腦梗死的發(fā)病率無相關(guān)性(P0.05)。3有高血壓病史的腦梗死組(HBP+CI)與無高血壓病史的腦梗死組(非HBP+CI)與相比,ORP-9兩個位點的基因型和等位基因頻率分布無統(tǒng)計學(xué)意義(P0.05);有糖尿病史的腦梗死組(DM+CI組)與無糖尿病史的腦梗死組(非DM+CI組)相比,ORP-9兩個位點的基因型和等位基因頻率的分布也無統(tǒng)計學(xué)意義(P0.05)。4在rs856600的C與T(CT+TT)和rs768529的A(AA+AT)與T的人群中的比較,血壓、空腹血糖、及TG、TC、HDL、LDL水平無統(tǒng)計學(xué)意義(P0.05)。5 rs856600和rs768529的基因型及等位基因頻率在WAS合并腦梗死、WAS的對照組中、AS合并腦梗死和AS的對照組中差異無統(tǒng)計學(xué)意義(P0.05);在VP合并腦梗死和SP合并腦梗死中,rs856600的基因型及等位基因頻率分布差異無統(tǒng)計學(xué)意義(P0.05),rs768529的基因型及等位基因頻率分布在VP合并腦梗死和SP合并腦梗死中差異有統(tǒng)計學(xué)意義(P0.05)。結(jié)論ORP-9的兩個位點的基因多態(tài)性跟河北地區(qū)人群腦梗死及影響腦梗死的危險因素如高血壓、高血糖及TG、TC、HDL、LDL等血脂因素可能不相關(guān)。rs856600的基因型及等位基因頻率跟AS及VP和SP不相關(guān),rs768529的T等位基因可能是河北地區(qū)漢族人群動脈粥樣硬化的易損斑塊的一個危險的因素。
[Abstract]:Objective to study the relationship between ORP-9 gene polymorphism and cerebral infarction by using steroid associated protein 9 (Oxysterol Binding Protein-related Protein-9,ORP-9 as a candidate gene for cerebral infarction, and to detect its distribution frequency in Hebei Han population. The relationship between Atherosclerosis (Atheroselerosis,AS), blood lipid, blood pressure and blood glucose was analyzed. Methods 174 patients with cerebral infarction from Hebei province were selected as cerebral infarction group, who were treated in affiliated Hospital of North China University of Science and Technology. A total of 117 healthy subjects from the same hospital without cerebral infarction or from the physical examination center of affiliated hospital of North China University of Technology were selected as the control group. The blood samples of the subjects were collected in the morning, and their medical history and related tests were followed up. The case control study was carried out with the control group. The extracted blood samples were extracted by whole blood extraction kit (DNA,) and then detected by PCR. The products amplified by PCR were analyzed by restriction fragment length polymorphism (Restriction Fragment Length Polymorphism,RFLP). Single nucleotide polymorphisms (SNP) of rs856600 and rs768529 at the two loci of ORP-9 were detected and their genotypes and alleles were detected. T test, chi-square test and Logistic regression model were used to process the statistical data, and the statistical data were analyzed by SPSS l 7.0 software. Results 1 in the comparison of the basic clinical data between the cerebral infarction group and the control group, there was no statistical significance between the two groups in many aspects, such as age, sex, smoking history and drinking history (P0.05). Compared with the control group, the proportion of body mass index, history of diabetes, history of hypertension, systolic blood pressure, diastolic blood pressure, fasting blood glucose, TG,TC and LDL were higher in cerebral infarction group (P0.05). However, the number of HDL in cerebral infarction was lower than that in control group (P0.05). The genotype and allele frequency of 2rs856600 and rs768529 had no significant difference between cerebral infarction and control group (P0.05). Their Logistic regression results, There was no correlation between the frequency of all genotypes and alleles and the incidence of cerebral infarction (P0.05). 3 (HBP CI) in cerebral infarction group with history of hypertension and cerebral infarction without hypertension history (non-HBP CI) compared with non-hypertensive group), The frequency distribution of genotype and allele of two ORP-9 loci had no statistical significance (P0.05). The cerebral infarction group with diabetes history (DM CI group) was compared with the cerebral infarction group (non-diabetic DM CI group) with no diabetes history. The distribution of genotype and allele frequencies at ORP-9 loci was also not statistically significant (P0.05). 4 comparison of blood pressure, fasting blood glucose, and TG,TC, between C and T (CT TT) of rs856600 and A (AA AT) and T of rs768529 There was no significant difference in HDL,LDL level (P0.05). 5 the genotype and allele frequency of rs856600 and rs768529 were not significantly different in WAS with cerebral infarction, AS with cerebral infarction and AS (P0.05). There was no significant difference in the frequency distribution of rs856600 genotype and allele between VP with cerebral infarction and SP with cerebral infarction (P0.05). The genotypes and allelic frequencies of rs768529 were significantly different between VP with cerebral infarction and SP with cerebral infarction (P0.05). Conclusion the polymorphism of two loci of ORP-9 is associated with cerebral infarction and risk factors such as hypertension, hyperglycemia and TG,TC,HDL, in Hebei population. The genotype and allele frequency of rs856600 may not be associated with AS, VP and SP. The T allele of rs768529 may be a risk factor for atherosclerotic plaque in Hebei Han population.
【學(xué)位授予單位】：華北理工大學(xué)
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2017
【分類號】：R743.33
本文編號：2397640
[Abstract]:Objective to study the relationship between ORP-9 gene polymorphism and cerebral infarction by using steroid associated protein 9 (Oxysterol Binding Protein-related Protein-9,ORP-9 as a candidate gene for cerebral infarction, and to detect its distribution frequency in Hebei Han population. The relationship between Atherosclerosis (Atheroselerosis,AS), blood lipid, blood pressure and blood glucose was analyzed. Methods 174 patients with cerebral infarction from Hebei province were selected as cerebral infarction group, who were treated in affiliated Hospital of North China University of Science and Technology. A total of 117 healthy subjects from the same hospital without cerebral infarction or from the physical examination center of affiliated hospital of North China University of Technology were selected as the control group. The blood samples of the subjects were collected in the morning, and their medical history and related tests were followed up. The case control study was carried out with the control group. The extracted blood samples were extracted by whole blood extraction kit (DNA,) and then detected by PCR. The products amplified by PCR were analyzed by restriction fragment length polymorphism (Restriction Fragment Length Polymorphism,RFLP). Single nucleotide polymorphisms (SNP) of rs856600 and rs768529 at the two loci of ORP-9 were detected and their genotypes and alleles were detected. T test, chi-square test and Logistic regression model were used to process the statistical data, and the statistical data were analyzed by SPSS l 7.0 software. Results 1 in the comparison of the basic clinical data between the cerebral infarction group and the control group, there was no statistical significance between the two groups in many aspects, such as age, sex, smoking history and drinking history (P0.05). Compared with the control group, the proportion of body mass index, history of diabetes, history of hypertension, systolic blood pressure, diastolic blood pressure, fasting blood glucose, TG,TC and LDL were higher in cerebral infarction group (P0.05). However, the number of HDL in cerebral infarction was lower than that in control group (P0.05). The genotype and allele frequency of 2rs856600 and rs768529 had no significant difference between cerebral infarction and control group (P0.05). Their Logistic regression results, There was no correlation between the frequency of all genotypes and alleles and the incidence of cerebral infarction (P0.05). 3 (HBP CI) in cerebral infarction group with history of hypertension and cerebral infarction without hypertension history (non-HBP CI) compared with non-hypertensive group), The frequency distribution of genotype and allele of two ORP-9 loci had no statistical significance (P0.05). The cerebral infarction group with diabetes history (DM CI group) was compared with the cerebral infarction group (non-diabetic DM CI group) with no diabetes history. The distribution of genotype and allele frequencies at ORP-9 loci was also not statistically significant (P0.05). 4 comparison of blood pressure, fasting blood glucose, and TG,TC, between C and T (CT TT) of rs856600 and A (AA AT) and T of rs768529 There was no significant difference in HDL,LDL level (P0.05). 5 the genotype and allele frequency of rs856600 and rs768529 were not significantly different in WAS with cerebral infarction, AS with cerebral infarction and AS (P0.05). There was no significant difference in the frequency distribution of rs856600 genotype and allele between VP with cerebral infarction and SP with cerebral infarction (P0.05). The genotypes and allelic frequencies of rs768529 were significantly different between VP with cerebral infarction and SP with cerebral infarction (P0.05). Conclusion the polymorphism of two loci of ORP-9 is associated with cerebral infarction and risk factors such as hypertension, hyperglycemia and TG,TC,HDL, in Hebei population. The genotype and allele frequency of rs856600 may not be associated with AS, VP and SP. The T allele of rs768529 may be a risk factor for atherosclerotic plaque in Hebei Han population.
【學(xué)位授予單位】：華北理工大學(xué)
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2017
【分類號】：R743.33
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