【摘要】：目的探討胰島素樣生長因子-1（insulin-like growth factor-1, IGF-1）對缺氧缺血性腦損傷(hypoxic-ischemic brain damage, HIBD)新生小鼠腦組織線粒體Cyt-C（細胞色素C）、caspase-3（半胱氨酸天冬氨酸蛋白酶-3）表達的影響。 方法108只新生7日齡C57/BL小鼠隨機分為正常對照組、缺氧缺血組（HI）組、HI+IGF-1組。采用改良Rice方法制備新生小鼠右腦HIBD動物模型，HI+IGF-1組于HI后即刻給予腹腔注射IGF-1（50μg/Kg），HI組和正常對照組注射等體積生理鹽水。應(yīng)用RT-PCR和Western Blot檢測各組皮質(zhì)、丘腦、海馬0h、3h、6h、12h、24h及48h Cyt-C、caspase-3mRNA和蛋白的表達。。 結(jié)果： ①HI組Cyt-C、caspase-3mRNA及蛋白表達明顯增加出現(xiàn)在皮質(zhì)術(shù)后3h，海馬術(shù)后6h，丘腦出現(xiàn)在術(shù)后12h，明顯遲于皮質(zhì)及海馬；②H I+IGF-1組Cyt-C、caspase-3mRNA及蛋白表達在HI組表達明顯增加的相應(yīng)時間點雖較正常對照組升高，但較HI組表達明顯降低，三組之間比較差異有統(tǒng)計學(xué)意義（P0.05）；③H I組皮質(zhì)Cyt-C、caspase-3mRNA及蛋白表達于術(shù)后3h表達明顯增加，6h有所下降，，12-24h達到另一高峰，峰值時間點HI+IGF-1組的表達較HI組均降低（P0.05）。 結(jié)論IGF-1可能通過抑制Cyt-C釋放，減少caspase-3表達，發(fā)揮對新生小鼠HIBD的神經(jīng)保護作用，IGF-1對皮質(zhì)的保護有兩個時間點。
[Abstract]:Objective to investigate the effect of insulin-like growth factor-1 (insulin-like growth factor-1, IGF-1) on mitochondrial Cyt-C (cytochrome C),) in brain tissue of hypoxic-ischemic brain injury (hypoxic-ischemic brain damage, HIBD) mice. Effect of caspase-3 (cysteine aspartate protease-3) expression. Methods 108 7 day old C57/BL mice were randomly divided into normal control group, (HI) group and HI IGF-1 group. The right brain HIBD model of newborn mice was established by modified Rice method. HI IGF-1 group was injected intraperitoneally with IGF-1 (50 渭 g/Kg), HI group and normal control group) immediately after HI. RT-PCR and Western Blot were used to detect the expression of Cyt-C,caspase-3mRNA and protein in cortex, thalamus and hippocampus. Results: the expression of Cyt-C,caspase-3mRNA and protein increased significantly in 1HI group at 3 hours after cortical operation, 6 hours after hippocampus operation and 12 hours after operation of thalamus, which was significantly later than that of cortex and hippocampus. 2The expression of Cyt-C,caspase-3mRNA and protein in the HI group was significantly higher than that in the normal control group, but lower than that in the HI group, and the difference between the three groups was statistically significant (P0.05). In 3H I group, the expression of Cyt-C,caspase-3mRNA and protein increased significantly at 3 h after operation, decreased at 6 h, reached another peak from 12 to 24 h, and the expression of HI IGF-1 at the peak time was lower than that in HI group (P0.05). Conclusion IGF-1 may play a neuroprotective role on HIBD in newborn mice by inhibiting the release of Cyt-C and decreasing the expression of caspase-3. IGF-1 has two time points for the protection of cortex.
【學(xué)位授予單位】：安徽醫(yī)科大學(xué)
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2014
【分類號】：R742

【參考文獻】

相關(guān)期刊論文 前10條

1 張雨平;趙聰敏;王麗雁;溫恩懿;于若谷;;caspase-3特異性肽類抑制劑抗新生鼠缺氧缺血腦損傷量效研究[J];重慶醫(yī)學(xué);2006年19期

2 李智勇;夏鷹;陳曉東;羅漢;;IGF-1對大鼠缺血、缺氧神經(jīng)元p-JNK及p-P38表達的影響[J];重慶醫(yī)學(xué);2012年16期

3 王楸,陳超,劉登禮,楊毅,陳蓮;胰島素樣生長因子-1(IGF-1)對缺氧缺血腦損傷新生鼠內(nèi)源性IGF-1和IGF-1受體基因表達的影響[J];中國當(dāng)代兒科雜志;2004年06期

4 黑明燕;黃維清;劉芙蓉;;rhIGF-1對缺氧缺血性腦損傷新生大鼠Cyt-C及caspase-3表達的影響[J];中國當(dāng)代兒科雜志;2010年12期

5 張雨平,趙聰敏,劉立,王麗雁,溫恩懿,廖偉;缺氧缺血新生鼠腦caspase-3活性的變化[J];第三軍醫(yī)大學(xué)學(xué)報;2003年13期

6 亢登峰;劉揚;王英元;王亞方;;HIF-1α、IGF-1表達與腦挫傷時間的相關(guān)性[J];法醫(yī)學(xué)雜志;2007年03期

7 李開榮;李旺輝;蕭洪文;楊朝鮮;;IGF-1對大鼠局灶性腦損傷c-fos表達的影響[J];中國實用神經(jīng)疾病雜志;2008年11期

8 湯軼波;張瑋;唐卉凌;李澎濤;;胰島素樣生長因子1對缺血缺氧神經(jīng)元的保護及其與PI3K信號轉(zhuǎn)導(dǎo)通路的關(guān)系(英文)[J];中南大學(xué)學(xué)報(醫(yī)學(xué)版);2011年01期

9 陳惠金,周澤漢,周建德,周偉,吳圣楣;新生大鼠缺氧實驗裝置的研制[J];上海實驗動物科學(xué);1999年01期

10 ;Human insulin-like growth factor 1-transfected umbilical cord blood neural stem cell transplantation improves hypoxic-ischemic brain injury[J];Neural Regeneration Research;2011年19期

本文編號：2304530