羥基紅花黃色素A對(duì)腦組織蛋白質(zhì)羰基化影響的體外研究
發(fā)布時(shí)間：2018-10-26 07:47
【摘要】：目的研究羥基紅花黃色素A(HSYA)對(duì)體外ONOO~-途徑和亞鐵血紅素/亞硝酸鈉/過(guò)氧化氫(heme/NaNO_2/H_2O_2)途徑引起腦組織蛋白質(zhì)羰基化的影響。方法分別模擬體內(nèi)ONOO~-、heme/NaNO_2/H_2O_2羰基化途徑,以腦組織蛋白為羰基化底物,分為空白對(duì)照組、對(duì)照組及低、高濃度組(以HSYA 0.1 mmol/L、1 mmol/L干預(yù))。以2、4-二硝基苯肼(2、4-dinitrophenylhydrazine,DNPH)法檢測(cè)蛋白質(zhì)羰基化水平。結(jié)果 ONOO~-途徑和heme/NaNO_2/H_2O_2途徑均可以增加腦組織勻漿中羰基蛋白的含量。HSYA預(yù)處理可濃度依賴性地降低ONOO~-途徑誘導(dǎo)的蛋白質(zhì)羰基化水平,低、高濃度組羰基蛋白含量分別降低了26.13%、46.23%,差異有統(tǒng)計(jì)學(xué)意義(F=14.265,P0.05)。HSYA預(yù)處理對(duì)體外heme/NaNO_2/H_2O_2途徑誘導(dǎo)的蛋白質(zhì)羰基化沒(méi)有明顯抑制作用,差異無(wú)統(tǒng)計(jì)學(xué)意義(P0.05)。結(jié)論 HSYA可劑量依賴性地抑制ONOO~-途徑在體外對(duì)腦組織蛋白的羰基化修飾;提示HSYA抑制蛋白質(zhì)羰基化反應(yīng)可能是其對(duì)抗腦血管疾病與神經(jīng)系統(tǒng)變性疾病的分子機(jī)制之一。
[Abstract]:Objective to study the effects of hydroxysafflor yellow A (HSYA) on the carbonylation of protein in brain tissue induced by ONOO~- pathway and hemin / sodium nitrite / hydrogen peroxide (heme/NaNO_2/H_2O_2) pathway in vitro. Methods ONOO~-,heme/NaNO_2/H_2O_2 carbonylation pathway was simulated in vivo, and brain tissue protein was used as carbonylation substrate. It was divided into blank control group, control group and low and high concentration groups (treated with HSYA 0.1 mmol/L,1 mmol/L). The level of carbonylation of protein was determined by DNPH method with 2o 4-dinitrophenylhydrazinehydrazinehydrazineine (DNPH). Results both ONOO~- pathway and heme/NaNO_2/H_2O_2 pathway could increase the content of carbonyl protein in brain homogenate, HSYA pretreatment could decrease the level of protein carbonylation induced by ONOO~- pathway in a concentration-dependent manner. The content of carbonyl protein in high concentration group decreased 26.13% and 46.23%, respectively, and the difference was statistically significant (F < 14.265, P < 0.05). P05). HSYA pretreatment had no significant inhibitory effect on protein carbonylation induced by heme/NaNO_2/H_2O_2 pathway in vitro, but the difference was not statistically significant (P0.05). Conclusion HSYA can inhibit the carbonylation modification of brain tissue proteins by ONOO~- pathway in vitro in a dose-dependent manner, suggesting that the inhibition of protein carbonylation by HSYA may be one of its molecular mechanisms against cerebrovascular diseases and neurodegenerative diseases.
【作者單位】： 河北省邢臺(tái)市人民醫(yī)院神經(jīng)內(nèi)二科;河北省邢臺(tái)市第三醫(yī)院神經(jīng)內(nèi)一科;天津醫(yī)科大學(xué)總醫(yī)院天津市神經(jīng)病學(xué)研究所;
【分類號(hào)】：R741
，
本文編號(hào)：2295042
[Abstract]:Objective to study the effects of hydroxysafflor yellow A (HSYA) on the carbonylation of protein in brain tissue induced by ONOO~- pathway and hemin / sodium nitrite / hydrogen peroxide (heme/NaNO_2/H_2O_2) pathway in vitro. Methods ONOO~-,heme/NaNO_2/H_2O_2 carbonylation pathway was simulated in vivo, and brain tissue protein was used as carbonylation substrate. It was divided into blank control group, control group and low and high concentration groups (treated with HSYA 0.1 mmol/L,1 mmol/L). The level of carbonylation of protein was determined by DNPH method with 2o 4-dinitrophenylhydrazinehydrazinehydrazineine (DNPH). Results both ONOO~- pathway and heme/NaNO_2/H_2O_2 pathway could increase the content of carbonyl protein in brain homogenate, HSYA pretreatment could decrease the level of protein carbonylation induced by ONOO~- pathway in a concentration-dependent manner. The content of carbonyl protein in high concentration group decreased 26.13% and 46.23%, respectively, and the difference was statistically significant (F < 14.265, P < 0.05). P05). HSYA pretreatment had no significant inhibitory effect on protein carbonylation induced by heme/NaNO_2/H_2O_2 pathway in vitro, but the difference was not statistically significant (P0.05). Conclusion HSYA can inhibit the carbonylation modification of brain tissue proteins by ONOO~- pathway in vitro in a dose-dependent manner, suggesting that the inhibition of protein carbonylation by HSYA may be one of its molecular mechanisms against cerebrovascular diseases and neurodegenerative diseases.
【作者單位】： 河北省邢臺(tái)市人民醫(yī)院神經(jīng)內(nèi)二科;河北省邢臺(tái)市第三醫(yī)院神經(jīng)內(nèi)一科;天津醫(yī)科大學(xué)總醫(yī)院天津市神經(jīng)病學(xué)研究所;
【分類號(hào)】：R741
，
本文編號(hào)：2295042
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