【摘要】：目的:了解感染所引起的新生大鼠感染性腦組織中Sox17和(髓鞘堿性磷酸酶)MBP的表達(dá)。方法:將64只2日齡SD大鼠隨機(jī)分為對照組、實(shí)驗(yàn)組。實(shí)驗(yàn)組生后第2~6 d持續(xù)腹腔注射脂多糖(LPS)每日0.6 mg/kg(0.01 rnl/g)。對照組則按照相同的注射方式及注射劑量,給予生理鹽水,建立新生大鼠腦白質(zhì)損傷模型。注射后按觀察時(shí)間點(diǎn)不同分為1 d、3 d、7 d、15 d,4個(gè)亞組(n=8)。處死大鼠取出腦白質(zhì),HE染色觀察腦白質(zhì)區(qū)病理變化,Western Blot法測定各組腦組織中Sox17和MBP蛋白的表達(dá),RT-RCR法檢測Sox17和MBPmRNA的表達(dá)變化。結(jié)果:實(shí)驗(yàn)組大鼠生長發(fā)育緩慢,HE染色顯示腦白質(zhì)區(qū)出現(xiàn)組織水腫,部分核固縮、核碎裂。PCR和Western Blotting結(jié)果 發(fā)現(xiàn)Sox17在生后1 d開始表達(dá),7 d達(dá)高峰,隨后下降,各時(shí)間點(diǎn)實(shí)驗(yàn)組與對照組相比差異有統(tǒng)計(jì)學(xué)意義(P0.05)。MBP在實(shí)驗(yàn)組的表達(dá)顯著下降,各時(shí)間點(diǎn)實(shí)驗(yàn)組與對照組相比差異有統(tǒng)計(jì)學(xué)意義(P0.05)。結(jié)論:生后感染可導(dǎo)致新生大鼠腦白質(zhì)損傷,MBP表達(dá)減少,Sox17表達(dá)明顯升高,提示Sox17劑量的升高對感染后新生大鼠腦組織可能具有保護(hù)性修復(fù)作用。
[Abstract]:Aim: to investigate the expression of Sox17 and (myelin alkaline phosphatase) MBP in infective brain tissues of neonatal rats induced by infection. Methods: 64 2-day old SD rats were randomly divided into control group and experimental group. Lipopolysaccharide (LPS) was continuously injected intraperitoneally on the 2nd day after birth of the experimental group for 0.6 mg/kg (0. 01 rnl/g) per day. In the control group, the white matter injury model of neonatal rats was established according to the same injection method and dose. After injection, the rats were divided into 4 subgroups (n = 8) according to different observation time points: 1 day, 3 days, 7 days, 15 days. The expression of Sox17 and MBP in brain tissue was detected by Western Blot method. The expression of Sox17 and MBP mRNA was detected by RT-RCR. Results: the slow growth and development of rats in the experimental group showed edema in the white matter area, partial nuclear contraction, nuclear fragmentation. PCR and Western blotting results showed that the expression of Sox17 reached its peak at 1 day after birth and then decreased. The expression of MBP in experimental group was significantly lower than that in control group at each time point (P0.05), and there was significant difference between experimental group and control group at each time point (P0.05). Conclusion: postnatal infection can reduce the expression of MBP and increase the expression of Sox17 in the white matter of neonatal rats, suggesting that the increase of the dose of Sox17 may have protective effect on the brain tissue of neonatal rats after infection.
【作者單位】： 徐州醫(yī)科大學(xué)附屬醫(yī)院新生兒科;

【相似文獻(xiàn)】

相關(guān)會議論文 前10條

1 尹音;孫振宇;胡敏;李冬霞;;持續(xù)性高正加速度對大鼠顳頜關(guān)節(jié)損傷的作用[A];第八屆全國顳下頜關(guān)節(jié)病學(xué)及（牙合）學(xué)大會論文匯編[C];2011年

2 祝~=驤;iJ梊霞;洃克琴;崔素英;文允摪;，

本文編號：2135373