本文選題：缺血缺氧 + 腦白質(zhì)損傷　； 參考：《神經(jīng)解剖學(xué)雜志》2017年06期

【摘要】：目的:觀察過(guò)表達(dá)特異性轉(zhuǎn)錄因子Olig2的少突膠質(zhì)前體細(xì)胞(oligodendrocyte precursor cells,OPCs)移植在缺血缺氧(hypoxia-ischemia,HI)腦白質(zhì)損傷新生大鼠腦內(nèi)的分化情況。方法:用綠色熒光蛋白(green fluorescent protein,GFP)標(biāo)記的過(guò)表達(dá)Olig2的慢病毒感染原代分離純化的大鼠大腦皮層OPCs,GFP陽(yáng)性細(xì)胞計(jì)數(shù)測(cè)其感染率。將過(guò)表達(dá)Olig2的OPCs(Olig2組)或陰性對(duì)照病毒感染的OPCs(Vector組)腦立體定位注射到造模后7 d的HI模型大鼠胼胝體膝部,移植后2周,冰凍切片行免疫熒光染色觀察OPCs的存活和分化情況。結(jié)果:Olig2-GV218病毒感染OPCs細(xì)胞48 h,熒光顯微鏡檢測(cè)顯示85%左右的OPCs細(xì)胞表達(dá)GFP;移植后2周,caspase-3熒光染色表明移植后絕大部分細(xì)胞存活,Vector組和Olig2組之間無(wú)統(tǒng)計(jì)學(xué)差異(P0.05);GFAP/GFP雙陽(yáng)性細(xì)胞在兩組之間也無(wú)顯著差異(P0.05);而Olig2組MBP/GFP雙陽(yáng)性細(xì)胞的熒光密度顯著高于Vector組(P0.05)。結(jié)論:過(guò)表達(dá)Olig2可促進(jìn)移植OPCs向少突膠質(zhì)細(xì)胞分化。
[Abstract]:Aim: to observe the differentiation of oligodendrocyte precursor cells with oligodendrocyte precursor cells in the white matter injury of neonatal rats after ischemia and hypoxia. Methods: the infection rate of lentivirus labeled with green fluorescent protein (GFP) was determined by primary isolation and purification of GFP positive cells from rat cerebral cortex. The rats were injected into the knees of corpus callosum of HI model rats 7 days after transplantation. The survival and differentiation of OPCs were observed by immunofluorescence staining in frozen sections 2 weeks after transplantation. Results at 48 h after infection with OPCs cells, about 85% of OPCs cells were detected by fluorescence microscopy, and 2 weeks after transplantation, there was no significant difference between the survival vector group and Olig2 group in the survival of most of the cells, and there was no significant difference between the two groups (P0.05GFP / GFAP / GFP). There was no significant difference in sex cells between the two groups, while the fluorescence density of MBP/GFP double positive cells in Olig2 group was significantly higher than that in Vector group. Conclusion: overexpression of Olig2 can promote the differentiation of OPCs into oligodendrocytes.
【作者單位】： 徐州醫(yī)科大學(xué)機(jī)能學(xué)實(shí)驗(yàn)中心;徐州醫(yī)科大學(xué)神經(jīng)生物學(xué)研究中心;
【基金】：國(guó)家自然科學(xué)基金(81271345)
【分類號(hào)】：R742
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本文編號(hào)：1971587