本文選題：坐骨神經(jīng)/損傷 + 神經(jīng)節(jié)�。� 參考：《安徽醫(yī)科大學(xué)》2014年博士論文

【摘要】：目的:神經(jīng)肽P物質(zhì)(Substance P SP)、降鈣素基因相關(guān)肽(Calcitonin gene-related peptide CGRP)共同存在于大鼠坐骨神經(jīng)損傷后背根神經(jīng)節(jié)中。本研究探討大鼠坐骨神經(jīng)損傷后背根神經(jīng)節(jié)內(nèi)神經(jīng)肽SP、CGRP陽性表達(dá)及意義,今后為進(jìn)一步探索周圍神經(jīng)損傷后神經(jīng)肽可能起的作用提供理論支持。方法:收集40只SD(成年雄性大鼠),其中20只SD制備大鼠左側(cè)坐骨神經(jīng)損傷模型組(A組);10只SD制備大鼠左側(cè)坐骨神經(jīng)離斷后吻合模型組(B組);同時收集SD 10只作為正常手術(shù)對照組(C組)。①術(shù)后第1w、第2w、第4w:觀察A組腰5背根神經(jīng)節(jié)中P物質(zhì)(SP)、降鈣素基因相關(guān)肽(CGRP)陽性細(xì)胞計數(shù)及平均光密度(Mean optical density MOD),并且與C組進(jìn)行比較。②術(shù)后第4w、12w:觀察B組腰5背根神經(jīng)節(jié)中SP、CGRP陽性細(xì)胞計數(shù)及平均光密度(MOD),并且與C組進(jìn)行比較。③術(shù)后第12w:觀察B組中的坐骨神經(jīng)進(jìn)行組織學(xué)觀察;觀察吻合后坐骨神經(jīng)的電生理變化;觀察B組SP、CGRP神經(jīng)纖維;蛋白基因產(chǎn)物9.5(protein gene product9.5,PGP9.5)神經(jīng)纖維。結(jié)果:20例大鼠左側(cè)坐骨神經(jīng)損傷模型組(A組)、10例大鼠左側(cè)坐骨神經(jīng)離斷后吻合模型組(B組)及10例正常對照手術(shù)組(C組)均進(jìn)入結(jié)果分析。①術(shù)后1 w:A組背根神經(jīng)節(jié)內(nèi)SP、CGRP的陽性細(xì)胞計數(shù)及MOD高于C組,兩者有顯著性差異(p㩳0.05)。②術(shù)后2 w:A組背根神經(jīng)節(jié)內(nèi)SP、CGRP的陽性細(xì)胞計數(shù)及MOD高于C組兩者有顯著性差異(p㩳0.05)。③術(shù)后4 w:A組背根神經(jīng)節(jié)內(nèi)SP、CGRP的陽性細(xì)胞計數(shù)及MOD與C組無明顯差異(p0.05)。④術(shù)后1w A組背根神經(jīng)節(jié)內(nèi)SP、CGRP的陽性細(xì)胞計數(shù)及MOD高于術(shù)后2w,兩者有顯著性差異(p㩳0.05)。⑤A組背根神經(jīng)節(jié)內(nèi)SP、CGRP的平均光密度在術(shù)后第1w達(dá)高峰,到第4w恢復(fù)正常水平。⑥B組在術(shù)后4w背根神經(jīng)節(jié)內(nèi)SP、CGRP的的陽性細(xì)胞計數(shù)及MOD低于C組,兩者有顯著性差異(p㩳0.05)。⑦B組在術(shù)后12w背根神經(jīng)節(jié)內(nèi)SP、CGRP的陽性表達(dá)與C組無明顯差異(p0.05)。⑧B組在術(shù)后第12w SP、CGRP及PGP9.5顯示免疫陽性神經(jīng)纖維,能觀察到SP、CGRP神經(jīng)纖維。B組12w顯示坐骨神經(jīng)功能指標(biāo)有很好的恢復(fù),說明再生神經(jīng)中存在SP、CGRP神經(jīng)纖維。結(jié)論:SP、CGRP均不同程度的存在于大鼠坐骨神經(jīng)損傷后背根神經(jīng)節(jié)中。大鼠坐骨神經(jīng)損傷后,背根神經(jīng)節(jié)中SP、CGRP的陽性表達(dá)在初期顯著增加,可以作為早期坐骨神經(jīng)損傷的一個指標(biāo)。SP、CGRP不僅參與神經(jīng)損傷信號傳導(dǎo),而且在大鼠坐骨神經(jīng)損傷再生修復(fù)中可能起調(diào)控作用。
[Abstract]:Objective: neuropeptide P (Substance P SP), calcitonin gene related peptide (Calcitonin gene-related peptide CGRP) co exist in the dorsal root ganglion of rat sciatic nerve injury. This study is to explore the positive expression and significance of neuropeptide SP, CGRP in the dorsal root ganglion of rat sciatic nerve injury. 40 SD (adult male rats) were collected, of which 20 SD were used to prepare the rat left sciatic nerve injury model group (group A); 10 SD rats were prepared for the left sciatic nerve anastomosis model group (group B), and 10 were collected as the normal operation control group (group C). (1) the postoperative 1W, 2W and 4w: observed P substance (SP), calcitonin gene related peptide (CGRP) positive cell count and mean light density (Mean optical density MOD) in the 5 dorsal root ganglion of group A, and compared with C group. Histological observation of the sciatic nerve in group B after 12w: was observed, and the electrophysiological changes of the sciatic nerve were observed after the anastomosis; the SP, CGRP nerve fiber, protein gene product 9.5 (protein gene product9.5, PGP9.5) nerve fibers were observed in group B. Results: the left sciatic nerve injury model group (A group) in the left side of the rats, and the left sciatic nerve of the rats in 10 cases were observed. After the postfracture anastomosis model group (group B) and 10 normal controls (group C), the results were analyzed. (1) the number of SP, CGRP positive cells in the dorsal root ganglion and MOD in the 1 w:A groups were significantly higher than those in the C group (P? 0.05). (2) the number of SP in the dorsal root ganglion in group 2 w:A after operation, CGRP positive cell count and greater MOD than those in the group were significantly higher. Difference (P? 0.05). (3) there was no significant difference in the number of SP, CGRP positive cells in the dorsal root ganglion and the MOD and C group in the 4 w:A group (P0.05). (P0.05). There was a significant difference in the count of SP, CGRP positive cells in the dorsal root ganglion and the MOD in the 1W A group after operation (0.05). At the peak, the normal level was restored at 4W. 6. In group B, the number of positive cells in the 4W dorsal root ganglion and the number of CGRP were lower than those in the C group. There was a significant difference between the two groups (P? 0.05). (P?) B group in the dorsal root ganglion of the 12W dorsal root, and there was no significant difference in the positive expression of CGRP. SP, CGRP nerve fiber.B group 12W showed that the function of the sciatic nerve was well restored, indicating the existence of SP, CGRP nerve fibers in the regenerative nerve. Conclusion: SP, CGRP all exist in the dorsal root ganglion of the sciatic nerve injury in rats. After the injury of the sciatic nerve in the rat, the positive of SP and CGRP in the dorsal root ganglion. The expression is significantly increased at the early stage, which can be used as an indicator of the early sciatic nerve injury,.SP. CGRP not only participates in the signal transduction of nerve injury, but also may play a regulatory role in the regeneration and repair of the sciatic nerve injury in rats.
【學(xué)位授予單位】：安徽醫(yī)科大學(xué)
【學(xué)位級別】：博士
【學(xué)位授予年份】：2014
【分類號】：R745.4

【參考文獻(xiàn)】

相關(guān)期刊論文 前6條

1 吳仲敏;倪晶晶;凌樹才;;背根神經(jīng)節(jié)內(nèi)P物質(zhì)、降鈣素基因相關(guān)肽免疫陽性神經(jīng)元與陰莖包皮系帶感覺信息的傳遞[J];中華男科學(xué)雜志;2007年12期

2 吳利平;張華;李輝;李云慶;;大鼠臂旁核內(nèi)5-羥色胺陽性纖維和終末的來源[J];神經(jīng)解剖學(xué)雜志;2009年06期

3 楊藝敏;張穎;馮加純;;成年神經(jīng)再生機(jī)制研究進(jìn)展[J];山東醫(yī)藥;2009年49期

4 周彪;韓澤民;;降鈣素基因相關(guān)肽在骨修復(fù)和骨重建中的作用研究現(xiàn)狀[J];武警后勤學(xué)院學(xué)報(醫(yī)學(xué)版);2012年02期

5 陳健;尹宗生;張輝;胡勇;王偉;鐘林;;促紅細(xì)胞生成素對大鼠坐骨神經(jīng)損傷后背根神經(jīng)節(jié)細(xì)胞Bcl-2和Bax表達(dá)的影響[J];安徽醫(yī)科大學(xué)學(xué)報;2011年09期

6 劉成龍;呂海;邱素均;段揚(yáng);;降鈣素基因相關(guān)肽表達(dá)載體的構(gòu)建[J];中國組織工程研究;2012年28期

，

本文編號：1962831