本文選題：癲癇 + 皮層發(fā)育障礙��； 參考：《中南大學(xué)》2014年碩士論文

【摘要】：目的本研究通過建立皮層發(fā)育不良模型與匹羅卡品癲癇大鼠模型,檢測皮層發(fā)育障礙致癇的大鼠及匹羅卡品癲癇大鼠模型中海馬區(qū)EphrinB3蛋白的表達(dá)變化,探討其在皮層發(fā)育不良致癇過程中的作用。 方法1.卡莫司汀誘導(dǎo)皮層發(fā)育障礙大鼠模型的建立及評估：10只SD孕鼠在懷孕17(E17)天腹腔內(nèi)注入卡莫司汀(Carmustine, BCNU)(15mg/Kg),所產(chǎn)仔鼠入模型組；9只SD孕鼠在E17天腹腔內(nèi)注入5%葡萄糖水(15mg/Kg),所產(chǎn)仔鼠入對照組。觀察皮層發(fā)育畸形仔鼠生長發(fā)育、腦濕重及仔鼠腦組織蘇木素-伊紅(HE)染色。 2.匹羅卡品癲癇模型的建立：隨機(jī)從皮層發(fā)育不良組及對照組抽取P60天雄鼠建立匹羅卡品癲癇模型,制備皮層發(fā)育障礙癲癇組(MCD+EP組)、普通大鼠癲癇組(EP組)、皮層發(fā)育障礙組(MCD組)及對照組(CON組)。 3.分別比較模型組和對照組大鼠的癲癇發(fā)生的潛伏期、癲癇持續(xù)狀態(tài)(SE)持續(xù)的時(shí)間及癲癇發(fā)生的死亡率,評估兩組大鼠的癲癇易感性。 4.采用免疫組化、免疫熒光及Real-time PCR分析方法檢測各組在SE誘導(dǎo)成功后1天(急性期)、14天(靜止期)及60天(慢性期)三個(gè)時(shí)間點(diǎn)大鼠海馬齒狀回中EphrinB3蛋白及mRNA的表達(dá)變化。 結(jié)果1.皮層發(fā)育障礙模型仔鼠一般狀態(tài)較對照組差。腦組織HE染色示模型組仔鼠的大腦皮質(zhì)變薄、皮質(zhì)及海馬層狀結(jié)構(gòu)紊亂,皮質(zhì)發(fā)育不良模型成功。 2.在MCD基礎(chǔ)上通過氯化鋰-匹羅卡品建立匹羅卡品癲癇大鼠動(dòng)物模型,建立皮層發(fā)育不良癲癇大鼠動(dòng)物模型。MCD模型鼠注射匹羅卡品后,SE發(fā)生的潛伏期顯著縮短(P0.05),癲癇持續(xù)狀態(tài)時(shí)間延長(P0.01),成功率及死亡率增加(P0.01),提示皮層發(fā)育障礙大鼠的癲癇易感性增加。 3.免疫組化與免疫熒光結(jié)果表明EphrinB3蛋白主要分布于海馬齒狀回；與EP組比較,MCD+EP組的EphrinB3在海馬齒狀回的表達(dá)顯著上調(diào),差異具有顯著性(P0.01),并在急性期、靜止期表達(dá)下調(diào),慢性期逐漸表達(dá)上調(diào)。與CON組比較,EP組在慢性期的EphrinB3表達(dá)也顯著上調(diào)(P0.01)。 4. Real-time PCR結(jié)果表明MCD+EP組EphrinB3mRNA的表達(dá)水平較EP組明顯增高,并在急性期、靜止期表達(dá)下調(diào),慢性期表達(dá)逐漸上調(diào)。 結(jié)論1. EphrinB3蛋白可能參與了皮層發(fā)育障礙大鼠海馬區(qū)異常神經(jīng)網(wǎng)絡(luò)的興奮性調(diào)節(jié)。 2. EphrinB3蛋白可能使皮層發(fā)育障礙大鼠對癲癇的易感性增高。
[Abstract]:Objective to study the changes of EphrinB3 protein expression in hippocampus of epileptic rats induced by cortical dysplasia and pilocarpine epileptic rats by establishing cortical dysplasia model and pilocarpine epileptic rat model. To explore its role in the process of eclampsia caused by cortical dysplasia. Method 1. Establishment and Evaluation of Cumoxetin-induced Cortical dysplasia in Rats: 10 Sprague-Dawley pregnant mice were intraperitoneally injected with Carmustine Carmosine and 15 mg / kg KgCU on gestational day 17. 9 SD pregnant rats in the model group were injected with 5% glucose water 15 mg / kg / L intraperitoneally on 17 days after gestation. The pups were added into the control group. Growth and development, brain wet weight and hematoxylin-eosin (HEH) staining were observed. 2. Establishment of pilocarpine epilepsy model: P60 day male rats were randomly selected from cortical dysplasia group and control group to establish pilocarpine epilepsy model. Preparation of cortical dysplasia epilepsy group MCD EP group, normal rat epilepsy group, cortex dysplasia group (MCD group) and control group (Con group). 3. The latency, duration and mortality of epileptogenesis were compared between the model group and the control group, and the susceptibility to epilepsy of the two groups was evaluated. 4. Immunohistochemistry, immunofluorescence and Real-time PCR analysis were used to detect the expression of EphrinB3 protein and mRNA in dentate gyrus of rats at 1 day after SE induction (14 days in acute phase (stationary phase) and 60 days in chronic phase). Result 1. The general state of the model mice with cortical dysplasia was worse than that of the control group. Brain tissue HE staining showed that cerebral cortex thinned, cortex and hippocampus lamellar structure disorder, cortical dysplasia model was successful in the model group. 2. On the basis of MCD, a rat model of pilocarpine epilepsy was established by lithium-pilocarpine chloride. Establishment of Cortical dysplasia of Epilepsy Rat Model .MCD Model Rats after injection of pilocarpine, the latency of SE was significantly shortened, the duration of epileptic status was prolonged, and the success rate and mortality were increased (P 0.01), indicating that the rats with cortical dysplasia had a significant increase in the latency of SE, which indicated that the rats with cortical dysplasia had a significant increase in the rate of success and mortality. Increased susceptibility to epilepsy. 3. Immunohistochemical and immunofluorescence results showed that EphrinB3 protein was mainly distributed in the dentate gyrus of Yu Hai horses, and the expression of EphrinB3 in the dentate gyrus was significantly up-regulated in the MCDEP group compared with the EP group, the difference was significant (P 0.01), and the expression of MCDEP was down-regulated in the acute phase and the stationary phase. The expression of chronic phase was gradually up-regulated. Compared with CON group, the expression of EphrinB3 was also significantly up-regulated in EP-treated group in chronic phase. 4. The results of Real-time PCR showed that the expression of EphrinB3mRNA in MCD EP group was significantly higher than that in EP group, and the expression of EphrinB3mRNA was down-regulated in acute phase, resting stage, and gradually up-regulated in chronic phase. Conclusion 1. EphrinB3 protein may be involved in excitatory regulation of abnormal neural network in hippocampus of rats with cortical dysplasia. 2. EphrinB3 protein may increase the susceptibility to epilepsy in rats with cortical dysplasia.
【學(xué)位授予單位】：中南大學(xué)
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2014
【分類號】：R742.1

【共引文獻(xiàn)】

相關(guān)期刊論文 前5條

1 于美娟,劉學(xué)伍;皮層發(fā)育不良與癲癇發(fā)病關(guān)系的研究[J];國外醫(yī)學(xué).神經(jīng)病學(xué)神經(jīng)外科學(xué)分冊;2003年05期

2 Ziyong Ju;Huashun Cui;Xiaohui Guo;Huayuan Yang;Jinsen He;Ke Wang;;Molecular mechanisms underlying the effects of acupuncture on neuropathic pain[J];Neural Regeneration Research;2013年25期

3 保承軍;岳桂杰;谷莉;;基于腦科學(xué)視角的建構(gòu)主義學(xué)習(xí)模式[J];太原大學(xué)教育學(xué)院學(xué)報(bào);2014年01期

4 楊光;鄒麗萍;;癲癇表觀遺傳學(xué)研究進(jìn)展[J];中國實(shí)用兒科雜志;2012年11期

5 吳梓齊;楊濤源;王景云;;淺談EphrinB2/ephB4在骨重建中的作用[J];中華老年口腔醫(yī)學(xué)雜志;2014年03期

相關(guān)博士學(xué)位論文 前8條

1 王亮;耐藥性癲癇分子病理機(jī)制及生物標(biāo)記物相關(guān)研究[D];重慶醫(yī)科大學(xué);2011年

2 朱瓊;DNA甲基化在耐藥性癲癇形成中的作用及可能機(jī)制[D];重慶醫(yī)科大學(xué);2012年

3 耿丹丹;EphB2對Aβ寡聚體誘導(dǎo)的神經(jīng)毒性損傷和NMDAR信號通路的保護(hù)作用[D];河北醫(yī)科大學(xué);2013年

4 蔣俊;在hTau小鼠及I型糖尿病小鼠中激活EphB2受體降低tau磷酸化的機(jī)制研究[D];華中科技大學(xué);2013年

5 孫丹妮;皮質(zhì)發(fā)育畸形大鼠皮質(zhì)神經(jīng)元AIS的可塑性研究[D];中南大學(xué);2013年

6 武江;人腦顳葉局灶性皮層發(fā)育不良臨床特征及發(fā)病機(jī)制研究[D];河北醫(yī)科大學(xué);2014年

7 吳尤佳;EphA5在先天性甲狀腺功能減低大鼠腦中的表達(dá)及DNA甲基化調(diào)控[D];蘇州大學(xué);2014年

8 劉岳鵬;脊髓ephrinB-EphB信號通路在慢性疼痛和嗎啡耐受/戒斷中的作用[D];第四軍醫(yī)大學(xué);2013年

相關(guān)碩士學(xué)位論文 前10條

1 李曉曉;骨髓間充質(zhì)干細(xì)胞移植對慢性腦缺血大鼠認(rèn)知功能及海馬區(qū)EphB2的影響[D];鄭州大學(xué);2013年

2 崔中水;EMT轉(zhuǎn)錄因子Twistl在肝癌血管生成擬態(tài)形成中差異microRNA的篩選[D];天津醫(yī)科大學(xué);2013年

3 張珍華;BDNF在FCDⅡa型癲癇灶中的表達(dá)、分布及意義[D];福建醫(yī)科大學(xué);2013年

4 程城;人參皂苷Rg3對腎癌786-0細(xì)胞中EphB4及Bcl-Xl蛋白的作用研究[D];南昌大學(xué)醫(yī)學(xué)院;2013年

5 杜陽;Reelin在皮質(zhì)發(fā)育障礙癲癇大鼠海馬區(qū)表達(dá)變化的研究[D];中南大學(xué);2013年

6 盧燦;Girdin在原發(fā)性肝細(xì)胞癌中的表達(dá)及其對肝癌細(xì)胞增殖和侵襲能力的影響[D];中南大學(xué);2013年

7 梁小敏;REM期睡眠剝奪對大鼠空間學(xué)習(xí)記憶以及海馬神經(jīng)發(fā)生的影響[D];第三軍醫(yī)大學(xué);2013年

8 陳瑜;EphA2及其S897位點(diǎn)磷酸化在鼻咽癌細(xì)胞生長和遷移中的作用[D];中南大學(xué);2013年

9 劉芳;高壓氧對局灶性腦缺血再灌注成年大鼠海馬齒狀回神經(jīng)干細(xì)胞增殖遷移分化的影響[D];華中科技大學(xué);2013年

10 李軍;血清B族維生素含量與癲癇相關(guān)性分析[D];山東大學(xué);2014年

，

本文編號：1950194