本文選題：樹突狀細胞 + OY-TES-��； 參考：《華中科技大學學報(醫(yī)學版)》2017年06期

【摘要】：目的研究負載癌-睪丸抗原(cancer-testis antigen,CTA)OY-TES-1的樹突狀細胞(dendritic cell,DC)體外抗膠質瘤的細胞免疫效應。方法細胞因子聯(lián)合誘導HLA-A2+健康人外周血單核細胞為DC,采用OY-TES-1重組蛋白致敏DC,流式細胞術檢測成熟DC表型分子;進而使DC誘導同體T淋巴細胞產生細胞毒性T淋巴細胞(cytotoxic T lymphocyte,CTL),CCK8檢測T細胞增殖情況,ELISPOT檢測IFN-γ含量,乳酸脫氫酶釋放法檢測CTL對膠質瘤細胞U251和A172的殺傷作用。結果負載OY-TES-1的DC低表達CD14,而高表達CD1a、CD83和CD80表型分子,能明顯促進CD8+T細胞的增殖及其IFN-γ的分泌(P0.05);而OY-TES-1特異性CTL在效靶比為50∶1時對HLA-A2+和OY-TES-1+U251、HLA-A2-和OY-TES-1+A172均有明顯殺傷作用(均P0.05),但其對U251的殺傷作用更強。結論體外用OY-TES-1重組蛋白致敏的DC可誘導特異性抗膠質瘤免疫應答,提示OY-TES-1可能成為膠質瘤免疫治療的靶點。
[Abstract]:Objective to study the effect of dendritic cells loaded with cancer-testis antigen1 (CTAOY-TES-1) on glioma in vitro. Methods HLA-A2 monocytes were induced by cytokines and OY-TES-1 recombinant protein was used to sensitize DC. Flow cytometry was used to detect the phenotypic molecules of mature DC. The cytotoxic T lymphocytes were induced by DC to produce cytotoxic T lymphocytes. The proliferation of T cells was detected by CCK8 and IFN- 緯 by Elispot. The cytotoxicity of CTL on U251 and A172 glioma cells was detected by lactate dehydrogenase release assay. Results the expression of CD14 was low in DC loaded with OY-TES-1, while the phenotypic molecules of CD1a, CD83 and CD80 were highly expressed in DC. It could obviously promote the proliferation of CD8 T cells and the secretion of IFN- 緯, while OY-TES-1 specific CTL could kill both HLA-A2 and OY-TES-1 U251 HLA-A2- and OY-TES-1 A172 at 50:1. Conclusion DC sensitized with OY-TES-1 recombinant protein in vitro can induce specific anti-glioma immune response, suggesting that OY-TES-1 may be the target of glioma immunotherapy.
【作者單位】： 廣西醫(yī)科大學第一附屬醫(yī)院神經外科;廣西醫(yī)科大學組織學與胚胎學教研室;廣西醫(yī)科大學基礎醫(yī)學研究重點實驗室;
【基金】：國家自然科學基金資助項目(No.81360371,No.81360374,No.81460382,No.81560408) 廣西自然科學基金資助項目(No.2016GXNSFAA380257,No.GXNSFBA380159) 廣西生物靶向診治研究重點實驗室開放課題(No.GXSWBX201505) 廣西區(qū)域性高發(fā)腫瘤早期防治研究重點實驗室自主研究課題(GKE2015-ZZ02)
【分類號】：R739.41
，

本文編號：1785199