本文選題：多發(fā)性硬化 + B淋巴細(xì)胞��； 參考：《蚌埠醫(yī)學(xué)院》2017年碩士論文

【摘要】：目的通過檢測多發(fā)性硬化患者與正常對照組調(diào)節(jié)性B細(xì)胞等相關(guān)B淋巴細(xì)胞占總B淋巴細(xì)胞百分率差異,并且分析患者不同臨床嚴(yán)重度與調(diào)節(jié)性B細(xì)胞的相關(guān)性,探討各B淋巴細(xì)胞亞群在多發(fā)性硬化中的潛在作用。方法收集從2016年7月到2017年3月入蚌埠醫(yī)學(xué)院第一附屬醫(yī)院神經(jīng)內(nèi)科住院的多發(fā)性硬化患者(n=12,男8例,女4例,年齡41.42±4.53歲)及同期門診體檢的無神經(jīng)系統(tǒng)或免疫系統(tǒng)疾病的健康者(n=12,性別、年齡均與多發(fā)性硬化組匹配)外周靜脈血樣本,并對所有多發(fā)性硬化患者進(jìn)行EDSS傷殘評分。采用流式細(xì)胞儀檢測患者及對照組外周靜脈血中CD19+CD24hi CD38hi細(xì)胞、CD19+CD27-CD38hi細(xì)胞、CD19+CD27hi CD38hi細(xì)胞占B淋巴細(xì)胞總數(shù)的百分率,酶聯(lián)免疫吸附測定患者及對照組外周靜脈血中IL-10水平。分析比較兩組的B淋巴細(xì)胞亞群差異及MS組CD19+CD24hi CD38hi細(xì)胞比例與EDSS是否具有線性相關(guān)。結(jié)果多發(fā)性硬化患者外周血調(diào)節(jié)性B細(xì)胞(CD19+CD24hi CD38hi)及過渡型B細(xì)胞(CD19+CD27-CD38hi)占總B淋巴細(xì)胞的百分率及IL-10水平與對照組之間差異有統(tǒng)計學(xué)意義(P0.05),多發(fā)性硬化患者外周血調(diào)節(jié)性B細(xì)胞、過渡型B細(xì)胞及IL-10水平均降低。多發(fā)性硬化患者漿母細(xì)胞(CD19+CD27hi CD38hi)占總B淋巴細(xì)胞的百分率與對照組相比無統(tǒng)計學(xué)意義(P0.05)。臨床嚴(yán)重性EDSS評分不同的多發(fā)性硬化患者與其調(diào)節(jié)性B細(xì)胞表達(dá)率無統(tǒng)計學(xué)差異(P0.05)。結(jié)論MS患者發(fā)病期調(diào)節(jié)性B細(xì)胞及過渡型B細(xì)胞表達(dá)降低伴隨IL-10減少,B淋巴細(xì)胞參與多發(fā)性硬化的發(fā)病。調(diào)節(jié)性B細(xì)胞表達(dá)降低與臨床嚴(yán)重度可能無關(guān)。
[Abstract]:Objective to detect the percentage of regulatory B lymphocytes in the patients with multiple sclerosis (MS) and normal controls, and to analyze the correlation between the clinical severity and the regulatory B cells in patients with multiple sclerosis. To explore the potential role of B lymphocyte subsets in multiple sclerosis. Methods from July 2016 to March 2017, 12 patients with multiple sclerosis, 8 males and 4 females, were admitted to the Department of Neurology, first affiliated Hospital of Bengbu Medical College. Age 41.42 鹵4.53 years old) and healthy subjects with no neurological or immune system diseases in the same period of outpatient examination, sex and age matched with multiple sclerosis group) peripheral venous blood samples were collected, and all patients with multiple sclerosis were evaluated with EDSS disability score. The percentage of CD19 CD24hi CD38hi cells, CD19 CD27hi CD38hi cells in peripheral venous blood of patients and control group was detected by flow cytometry, and the level of IL-10 in peripheral vein blood of patients and control group was measured by enzyme-linked immunosorbent assay (Elisa). The difference of B lymphocyte subsets between the two groups and whether the proportion of CD19 CD24hi CD38hi cells in MS group were linearly correlated with EDSS were analyzed and compared. Results the percentage of CD19 CD24hi CD38hiand the percentage of CD19 CD27-CD38hi1 in total B lymphocytes and the level of IL-10 in peripheral blood of patients with multiple sclerosis were significantly different from those of the control group (P 0.05). The levels of transitional B cells and IL-10 were decreased. The percentage of CD19 CD27hi CD38hi1 in total B lymphocytes in patients with multiple sclerosis was not significantly higher than that in control group (P 0.05). There was no significant difference in the expression rate of regulatory B cells between multiple sclerosis patients with different clinical severity EDSS scores (P 0.05). Conclusion the decreased expression of regulatory B cells and transitional B cells in MS patients is associated with the decrease of IL-10 B lymphocytes in the pathogenesis of multiple sclerosis. The decrease of regulatory B cell expression may not be associated with clinical severity.
【學(xué)位授予單位】：蚌埠醫(yī)學(xué)院
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2017
【分類號】：R744.51

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